RESUMO
OBJECTIVE: To establish an HPLC-ELSD method for determination of mesaconine. METHODS: Gemini C18 110A column (4.6 mm×150 mm, 5 μm) was adopted with the mobile phase consisting of methanol-0.1%TFA (25:75) at the flow rate of 0.6 mL·min-1. The detector was Alltech ELSD 2000ES, with the drift tube temperature of 90℃ and the airflow velocity of 2.3 L·min-1. RESULTS: Under this conditions, mesaconine and the related substances were well separated. The linear range of mesaconine was 0.40-0.60 mg·mL-1 (r=0.9996). RSD of injection precision and repeatability test were 0.04% (n=5) and 0.7% (n=6), the intra day and inter day precision RSD were 0.4% and 0.6%, respectively. The average recovery was 101. 0% (RSD=1.1%, n=9). CONCLUSION: The established method was specific, simple, accurate, and suitable for determination of mesaconine.