Brugia malayi abundant larval transcript 2 protein treatment attenuates experimentally-induced colitis in mice.

Helminths are known to modulate host’s immunity by suppressing host protective pro-inflammatory responses. Such immunomodulatory effects have been experimentally shown to have therapeutic implications in immune mediated disorders. In the present study, we have explored a filarial protein i.e. Brugia malayi recombinant abundant larval transcript 2 (rBmALT2) for its therapeutic effect in dextran sodium sulfate (DSS) induced colitis in mouse model. The immunomodulatory activity of rBmALT-2 was initially confirmed by demonstrating that it suppressed the lipopolysaccharide (LPS) induced nitric oxide synthesis and down-regulated the expression of pro-inflammatory cytokines in vitro by peritoneal exudate cells of mice. Treatment with rBmALT2 reduced severity of colitis associated with significant reduction in weight loss, disease activity, colon damage, mucosal edema and histopathological score including myeloperoxidase activity in colon tissues. rBmALT2 was comparatively more effective in attenuation of colitis when used in the preventive mode than when used for curative purpose. The therapeutic effect of rBmALT2 was found to be associated with downregulation of IFN-γ, IL-6, IL-17 and upregulation of IL-10 cytokines. These results provide strong experimental evidence that BmALT2 could be a potential alternative therapeutic agent in colitis.

The anthropometric measurements of tibia.

Anthropometry provides scientific method and technique for taking various measurements in different geographic regions and races. The Tibia itself is a complex anatomic unit so anthropometric study was devised on the same. In the present study 60 (26 right and 34 left) intact adult tibia were obtained from the bone bank of Anatomy Department of MGIMS, Sevagram. For this purpose a digital vernier caliper, osteometric board and measuring tape were used. The study was aimed at determining measurements for obtaining Cross-Section Index in middle, Cnemicus Index and Length-Thickness Index for both right and left Tibia. The details of data obtained with relevant review of literature will be discussed. The mean of Cross Sectional index for Right tibias was 102.90± 22.78. Similarly mean of Cross Sectional index for left tibias was 124.31± 25.06. The mean of Cnemicus Index for Right tibias was 66.17 ± 10.68 and for left side these values came out to be 67.31 ± 7.35. These are not statistically significant. The mean of Length-Thickness Index were 24.21 ± 0.96 and 24.43 ± 1.78 for right and left Tibias respectively.

A review of the chronology of epiphyseal union in the bones at knee and ankle joint.

Determination of the age of an individual from the appearance and the fusion of the ossification centres is a well accepted fact in the field of medical and legal professions. The process of formation of bones is called as “ossification”. Ossification of bone is a diagnostic tool for estimation of age until the process is complete for the particular bone. The long bones of lower limb play a vital role in assessment of age both in living and dry remains. Many scientists have tried to conclude there observations on bones of lower limb owing to differences in regional and environmental factors. Countable differences are noticed in the appearance and fusion activities of ossification centers depending on race geographic distribution and sex. Following is a review of works carried out so far in different regions of country as well as throughout the world.

Age determination in girls of Jodhpur Region by epiphyseal union of bones at ankle joint.

There is no statistical data to establish variation in epiphyseal fusion in Western Rajasthan populations. This significant oversight can lead to exclusion of persons of interest in a forensic investigation. Epiphyseal fusion of the distal tibia and fibula in sixty females was analyzed on radiological basis to assess the range of variation of epiphyseal fusion at each age. In the study the X ray films of the subjects were divided into three groups on the basis of degree of fusion. Firstly, those which were showing No Epiphyseal Fusion (N), secondly those showing Partial Union (P), and thirdly those showing Complete Fusion (C). Observations made were compared with the previous studies. Results indicate that complete fusion in females occurs as early as 14 years in the distal tibia and fibula. All females demonstrated complete fusion by 19 years with no significant differences between ancestral groups.