Fagonia cretica: Identification of compounds in bioactive gradient high performance liquid chromatography fractions against multidrug resistant human gut pathogens

@#Plants are alternative source of natural medicines due to secondary active metabolites. Fagonia cretica extracts and Gradient High-Pressure Liquid Chromatography fractionations were checked against multidrug-resistant gastrointestinal pathogens including, Salmonella typhi, Escherichia coli and Shigella flexneri. ESI-MS/MS analysis of bioactive HPLC fractions was performed to elucidate antibacterial compounds. F. cretica extracts exhibited potential antibacterial activity. Twenty-four (24) HPLC fractions were obtained from methanol, ethanol and aqueous extracts of F. cretica. Eighteen (18) fractions showed antibacterial activity, while no activity was observed by the remaining six (6) fractions. HPLC fractions, F1 (25g ± 0.20 mm) and F2 (15f ± 0.12 mm) of aqueous extract exhibited activity against multidrug resistant GI pathogens. Gallic acid, quinic acid, cyclo-l-leu-l-pro, vidalenolone, liquirtigenin, rosmarinic acid and cerebronic acid were identified in F1 fraction of aqueous extract, while succinic acid, cyclo (l-Leul-Pro) and liquirtigenin were identified in F2 fraction of aqueous extract through ESI-MS/MS analysis. F. cretica extracts and HPLC fractions showed potential activity against MDR GI pathogens. Vidalenolone, Cyclo-1-leu-1-pro and Cerebronic acid are first time reported in F. cretica. Further characterization of bioactive compounds from F. cretica may be helpful to elucidate antibacterial therapeutic molecules.

Ocular and orbital neoplasms in children

The study included 120 children presented with various ocular and orbital neoplasms. The incidence, the clinical presentations and the investigative findings were thoroughly assessed. The surgical techniques as well as the histopathological features were briefly discussed. The study revealed that retinoblastoma was the commonest primary intracular tumor 43%, followed by hemangioma 13%. Rhabdomyosarcoma was the third in order of frequency 8% and was the commonest primary intraorbital neoplasm

Management of aftercataract

The study included 142 cases of after-cataract in the different age groups. The procedures done were discission [8], scissors capsulectomy [21], automated membranectomy using the ocutome probe [29] and Nd:YAG capsulotomy [84]. The indications, difficulties and complications were mentioned and discussed

Aphakic retinal detachment

This study included 159 patients with aphakic retinal detachment [ARD] who were admitted to the Ophthalmic Department of Assiut University Hospital through a period of five years. Six patients had bilateral ARD. The type of cataract operation was intracapsular in 141 eyes and extracapsular in 24 eyes. Single retinal break was found in 17%, multiple breaks in 10% and in 28.5% no breaks could be detected. Conventional cryoretinopexy was performed in 124 eyes, vitrectomy in 10 eyes and 31 eyes were not operated upon

Perforating eye injury as an important cause of visual loss in Upper Egypt

It was noticed that perforating injuries of the eye are important problems causing loss of vision of one or both eyes. Thus a study of these injuries was done on one hundred and eighty six patients who where admitted to the ophthalmic department of Assiut University Hospital in a period of two years, aiming to study the causes, types, management and prognosis