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1.
Int. braz. j. urol ; 41(2): 373-378, Mar-Apr/2015. graf
Artigo em Inglês | LILACS | ID: lil-748286

RESUMO

Purpose Teaching the no-scalpel vasectomy is important, since vasectomy is a safe, simple, and cost-effective method of contraception. This minimally invasive vasectomy technique involves delivering the vas through the skin with specialized tools. This technique is associated with fewer complications than the traditional incisional vasectomy (1). One of the most challenging steps is the delivery of the vas through a small puncture in the scrotal skin, and there is a need for a realistic and inexpensive scrotal model for beginning learners to practice this step. Materials and Methods After careful observation using several scrotal models while teaching residents and senior trainees, we developed a simplified scrotal model that uses only three components–bicycle inner tube, latex tubing, and a Penrose drain. Results This model is remarkably realistic and allows learners to practice a challenging step in the no-scalpel vasectomy. The low cost and simple construction of the model allows wide dissemination of training in this important technique. Conclusions We propose a simple, inexpensive model that will enable learners to master the hand movements involved in delivering the vas through the skin while mitigating the risks of learning on patients. .


Assuntos
Humanos , Masculino , Modelos Anatômicos , Escroto/cirurgia , Vasectomia/educação , Vasectomia/métodos , Educação Médica/economia , Educação Médica/métodos , Reprodutibilidade dos Testes , Ensino/economia , Ensino/métodos , Ducto Deferente/cirurgia
2.
Afr. j. AIDS res. (Online) ; 13(3): 305-311, 2014.
Artigo em Inglês | AIM | ID: biblio-1256594

RESUMO

Understanding pregnancy planning and contraceptive use is important in preventing unplanned/unwanted pregnancies among women on antiretroviral therapy (ART). Through a cross-sectional survey of 155 women living with HIV on ART in Botswana (mean age = 36); bivariate/multivariate analyses were used to identify and understand pregnancy planning and contraceptive use. Women who did not plan to have a child (n = 85) were older; less educated; had more children and worried about stigmatisation from family and healthcare workers (HCWs). Multivariate analyses found age (OR:3.41; CI:1.57-7.45; p = 0.002); perceived stigmatisation from family and healthcare workers (OR:3.62; CI:1.47-8.96; p = 0.005); and believing it is irresponsible for women living with HIV to want a child (OR:2.40; CI:1.10-5.24; p = 0.028) to be significantly associated with not planning to have a child. Although reported condom use among 85 women who did not plan to have a child was nearly 90; a total of 26 of these women (34) believed they did not have control over condom use. Lack of contraception was reported by 6 women who did not plan a child; this; coupled with the lack of control over condom use; puts unmet need for contraception at 38 Most women reported feeling comfortable talking with HCWs about contraceptives. However; almost a quarter of the women indicated they were infrequently advised about contraceptives at ART clinics. This study found discordance between pregnancy planning and contraceptive use among women on ART. Lack of control over condom use coupled with low hormonal contraceptive use creates unmet need for contraception and increases the risk of unwanted pregnancies. Regular clinic visits for women on ART present excellent opportunities to address contraceptive needs in a considerate and comprehensive manner


Assuntos
Antirretrovirais/uso terapêutico , Terapia Antirretroviral de Alta Atividade , Botsuana , Preservativos/estatística & dados numéricos , Anticoncepcionais/administração & dosagem , Infecções por HIV/tratamento farmacológico , Gravidez , Comportamento Reprodutivo
3.
West Indian med. j ; 60(4): 434-441, June 2011. tab
Artigo em Inglês | LILACS | ID: lil-672807

RESUMO

OBJECTIVE: To determine the socio-economic determinants of obesity in adults in The Bahamas. DESIGN AND METHODS: A subpopulation of adults 21 to 60 years was analysed for socio-economic differences in obesity levels. Data from the 2001 Bahamas Living Conditions Survey, a nationwide comprehensive household survey which included anthropometric measurements, were used. Bivariate and binary logistic regression methods for complex samples were employed. FINDINGS: Overall obesity prevalence was 32% (38% female, 25% male, p = < 0.0001). An inverse relationship by education appeared to be the strongest predictor for all persons (OR = 0.78, CI 0.67, 0.90; p < 0.0001). This relationship was also evident for females (OR = 0.71, CI 0.59, 0.85; p< 0.0001) while a positive relationship existed by economic level for males (OR = 1.23, CI 1.07, 1.41; p = 0 .005). There was a difference in food group expenditure for starchy vegetables only (p = 0.049). Other food group household expenditure, urban residence and female headed households showed no significant differences by obesity. CONCLUSIONS: In line with international trends, obesity rates are high in The Bahamas, and especially affect females of lower socio-economic status. Public policy that targets this group is necessary to address this health concern.


OBJETIVO: Establecer cuáles son las determinantes socio-económicas de la obesidad en los adultos en Bahamas. DISEÑO Y MÉTODOS: Se analizó una subpoblación de adultos de 21 a 60 años en busca de las diferencias socio-económicas según los niveles de obesidad. Se usaron los datos de la Encuesta 2001 sobre las condiciones de vida en Bahamas - una encuesta general doméstica nacional que incluyó mediciones antropométricas. Se emplearon métodos de regresión logística bivariados y binarios para las muestras complejas. RESULTADOS: La prevalencia de obesidad global fue de 32% (38% hembras, 25% varones, p =< 0.0001). Una relación inversa para la educación pareció ser el predictor más fuerte para todas las personas (OR = 0.78, CI 0.67[N1], 0.90; p < 0.0001). Esta relación también se hizo evidente en las hembras (OR = 0.71, CI 0.59, 0.85; p < 0.0001) mientras que una relación positiva existió en el nivel económico para los varones (OR = 1.23, CI 1.07, 1.41; p = 0 .005). Hubo una diferencia en el gasto del grupo de alimentos en cuanto a verduras ricas en fécula solamente (p = 0.049). Otro gasto doméstico de grupo de alimentos, residencia urbana, y casas encabezadas por mujeres, no mostraron diferencias significativas en obesidad. CONCLUSIONES: En concordancia con las tendencias internacionales, las tasas de obesidad son altas en Bahamas, y afectan sobre todo a las mujeres de más bajo estatus socio-económico. Se necesitan políticas públicas dirigidas a este grupo, a fin de abordar este problema de salud.


Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Obesidade/epidemiologia , Bahamas/epidemiologia , Distribuição de Qui-Quadrado , Modelos Logísticos , Prevalência , Fatores Socioeconômicos
4.
Afr. j. AIDS res. (Online) ; 9(1): 11-16, 2010.
Artigo em Inglês | AIM | ID: biblio-1256730

RESUMO

This study investigates the experiences of doctors who perform elective surgical procedures in an area of South Africa with a high incidence of HIV and AIDS. A qualitative study approach was adopted. Interviews were individually conducted with 15 doctors. The transcribed interviews were read four times; each with a different data-collection purpose; and follow-up interviews were carried out when it was necessary to complete the data set. Surgical doctors from two semi-urban hospitals and one rural hospital in northern KwaZulu-Natal province took part in the study. The analysis of the interviews rendered four areas of concern in the experiences of doctors who perform surgery on HIV/AIDS patients. These were: personal factors; patient factors; factors relating to the structure of the health system; and factors concerning protocols for the treatment of patients with HIV or AIDS. Although the doctors were altruistic in their approaches to operating on HIV/AIDS patients; they commonly mentioned the increased levels of stress they experience as a result of a multiplicity of issues surrounding the treatment and care of an HIV/AIDS patient specifically. The public health system has not made special arrangements to deal with the increased patient loads in hospitals as a result of the HIV epidemic; and this will have to be addressed as the number of HIV/AIDS patients increases


Assuntos
Síndrome da Imunodeficiência Adquirida , Infecções por HIV , Procedimentos Cirúrgicos Operatórios
5.
S. Afr. fam. pract. (2004, Online) ; 52(2): 137-141, 2010.
Artigo em Inglês | AIM | ID: biblio-1269877

RESUMO

Background: Doctors need to constantly update their knowledge and obtain information in order to practise high-quality medicine. Antiretroviral drugs have been available only since around 1996; therefore many of the doctors who were trained prior to 1996 would not have received any formal training in the management of HIV and AIDS patients. Where doctors source their general medical knowledge has been established; but little is known about where doctors source information on HIV/AIDS. This study investigated where private sector doctors from the eThekwini Metro obtain information on HIV and AIDS for patient management. Methods: A descriptive cross-sectional study among 133 private general practitioners (GPs) and 33 specialist doctors in the eThekwini Metro of KwaZulu-Natal; South Africa; was conducted with the use of questionnaires. The questionnaires were analysed using SPSS version 15. A p value of 0.05 was considered statistically significant. Results: The majority of the doctors (92.4) obtained information on HIV and AIDS from journals. Continuing Medical Education (CME); textbooks; pharmaceutical representatives; workshops; colleagues and conferences were identified as other sources of information; while only 35.7of doctors were found to use the internet for information. GPs and specialists differed significantly with regard to their reliance on colleagues (52.9versus 72.7; p 0.05) and conferences (48.6versus 78.8; p 0.05) as sources of HIV information. More than 90of doctors reported that CME courses contributed to better management of HIV and AIDS patients.) obtained information on HIV and AIDS from journals. Continuing Medical Education (CME); textbooks; pharmaceutical representatives; workshops; colleagues and conferences were identified as other sources of information; while only 35.7of doctors were found to use the internet for information. GPs and specialists differed significantly with regard to their reliance on colleagues (52.9versus 72.7; p 0.05) and conferences (48.6versus 78.8; p 0.05) as sources of HIV information. More than 90of doctors reported that CME courses contributed to better management of HIV and AIDS patients. Conclusion: Private sector doctors in the eThekwini Metro obtain information on HIV from reliable sources in order to have up-to-date knowledge on the management of HIV-infected patients


Assuntos
HIV , Acesso à Informação , Síndrome da Imunodeficiência Adquirida , Médicos , Setor Privado
6.
S. Afr. fam. pract. (2004, Online) ; 52(5): 451-458, 2010.
Artigo em Inglês | AIM | ID: biblio-1269894

RESUMO

Background: Although private sector doctors are the backbone of treatment service in many countries; caring for patients with HIV entails a whole new set of challenges and difficulties. The few studies done on the quality of care of HIV patients; in the private sector in developing countries; have highlighted some problems with management. In South Africa; two-thirds of doctors work in the private sector. Though many studies on HIV/AIDS have been undertaken; few have been done in the private sector in terms of the management of this disease. Therefore; a study was undertaken to evaluate the clinical management of HIV-infected patients by private sector doctors. Methods: A descriptive cross-sectional study was undertaken in the eThekwini Metro in KwaZulu-Natal; South Africa; with 190 private sector doctors who; in the first phase of the study; indicated that they manage HIV and AIDS patients and would be willing to participate in the second phase of the study. The HIV guidelines of the Department of Health and Human Services and the South African National Department of Health were used to compare the treatment of HIV patients by these doctors. Results: Eighty-five doctors (54.5) always measured the CD4 count and viral load levels at diagnosis. Both CD4 counts and viral load were always used by 76 doctors (61.8) to initiate therapy. Of the doctors; 134 (78.5) initiated therapy at CD4 count 200 cells/mm3. The majority of doctors prescribed triple therapy regimens using the 2 NRTI + 1 NNRTI combination. Doctors who utilised CD4 counts tended to also use viral load (VL) to assess effectiveness and change therapy (p 0.001). At initiation of treatment; 68.5of the doctors saw their patients monthly and 64.3saw them every three to six months; when stable. Conclusion: The majority of private sector doctors were compliant with current guidelines for HIV management; hence maintaining an acceptable quality of clinical healthcare


Assuntos
Gerenciamento Clínico , Estudo de Avaliação , Infecções por HIV , Pacientes , Médicos , Setor Privado
7.
S. Afr. fam. pract. (2004, Online) ; 52(5): 471-475, 2010.
Artigo em Inglês | AIM | ID: biblio-1269898

RESUMO

Background: The danger of poor adherence to treatment by patients with HIV infection is that poor adherence correlates with clinical and virological failure. Understanding how private-sector doctors monitor adherence by their HIV-infected patients could assist in developing interventions to improve adherence by these patients. Information about such practices amongst private-sector doctors in the province of KwaZulu-Natal; however; is limited. This study was; therefore; undertaken to assess the private-sector doctor adherence-monitoring practices of HIV-infected patients in the eThekwini metro of KwaZulu-Natal. Methods: A descriptive cross-sectional study was undertaken amongst private general practitioners (GPs) and specialists managing HIV/AIDS patients in the eThekwini metro. Anonymous semi-structured questionnaires were used to investigate adherence-monitoring practices by these doctors and their strategies to improve adherence. Results: A total of 171 doctors responded; with over 75in practice for over 11 years and 78.9indicating that they monitored adherence. A comparison between the GPs and the specialists found that 82.6of the GPs monitored adherence compared with 63.6of the specialists (p = 0.016). The doctors used several approaches; with 60.6reporting the use of patient self-reports and 18.3reporting the use of pill counts. A total of 68.7of the doctors indicated that their adherence monitoring was reliable; whilst 19.7indicated that they did not test the reliability of their monitoring tools .The most common strategy used to improve adherence by their patients was through counselling. Other strategies included alarm clocks; SMSs; telephone calls to the patients; the encouragement of family support and the use of medical aid programmes. Conclusions: Private-sector doctors managing HIV/AIDS patients in the eThekwini metro of KwaZulu-Natal do monitor adherence and employ strategies to improve adherence


Assuntos
Síndrome da Imunodeficiência Adquirida , Gerenciamento Clínico , Infecções por HIV , Médicos , Setor Privado , Prática Profissional
8.
Artigo em Inglês | AIM | ID: biblio-1270646

RESUMO

There is increasing evidence of an association between female genital Schistosoma haematobium infection and HIV. In KwaZulu-Natal; we aimed to explore girls' water contact practice and to determine whether a study exclusively on girls would be manageable and welcomed. Three primary schools that had participated in a parasite control programme eight years prior were approached. Subject to consent; girls aged 9 to 12 years were interviewed on water-body contact; symptoms and household composition. Urine samples were analysed for S. haematobium infection eggs. Good dialogue was achieved in all schools and 95consented to participation; 43had an S. haematobium infection; geometric mean intensity 10.5 ova per 10 ml urine. Only 12had ever been treated for S. haematobium. Water-body contact was significantly associated with S. haematobium (OR 2.8; 95CI 1.3-5.9; p= 0.008); however; S. haematobium was also found in 20of girls who claimed to never have had water-body contact. Sixty-four percent thought they had no choice but to use unprotected water; 21had no mother in the household; and being an orphan increased the risk of having S. haematobium. The community welcomed the study. Prevalence levels in South Africa are so high that some communities are eligible for WHO-recommended regular mass treatment


Assuntos
Núcleo Familiar , Esquistossomose Urinária , Instituições Acadêmicas , Água
9.
West Indian med. j ; 58(5): 446-451, Nov. 2009. tab
Artigo em Inglês | LILACS | ID: lil-672519

RESUMO

This report describes the application of a draft version of the World Health Organization (WHO)/ United States Centers for Disease Control and Prevention (CDC) Manual for estimating the economic costs of injuries due to interpersonal and self-directed violence to measure costs of injuries from interpersonal violence. METHODS: Fatal incidence data was obtained from the Jamaica Constabulary Force. The incidence of nonfatal violence-related injuries that required hospitalization was estimated using data obtained from patients treated at and/or admitted to three Type A government hospitals in 2006. RESULTS: During 2006, direct medical cost (J$2.1 billion) of injuries due to interpersonal violence accounted for about 12% of Jamaica's total health budget while productivity losses due to violence-related injuries accounted for approximately J$27.5 billion or 160% of Jamaica's total health expenditure and 4% of Jamaica's Gross Domestic Product. CONCLUSIONS: The availability of accurate and reliable data of the highest quality from health-related information systems is critical for providing useful data on the burden of violence and injury to decision-makers. As Ministries of Health take a leading role in violence and injury prevention, data collection and information systems must have a central role. This study describes the results of one approach to examining the economic burden of interpersonal violence in developing countries where the burden of violence is heaviest. The WHO-CDC manual also tested in Thailand and Brazil is a first step towards generating a reference point for resource allocation, priority setting and prevention advocacy.


Este reporte describe la aplicación de una versión preliminar del Manual de Centros de Estados Unidos para el control y prevención de enfermedades (CDC)/Organización Mundial de la Salud (OMS), para estimar el costo económico de las heridas debidas a la violencia interpersonal y la violencia auto-dirigida, con el fin de evaluar los costos de las heridas por violencia interpersonal. MÉTODOS: Datos sobre las incidencias fatales fueron obtenidos de las Oficinas de la Policía de Jamaica. La incidencia de las heridas no fatales relacionadas con la violencia, pero que no obstante requirieron hospitalización, se calculó a partir de pacientes tratados o ingresados en hospitales gubernamentales del tipo A, en el año 2006. RESULTADOS: Durante el 2006, el costo médico directo (2.1 billones JMD) por heridas a causa de violencia interpersonal, representó alrededor del 12% del total del presupuesto para la salud en Jamaica, mientras que las pérdidas de productividad debido a heridas relacionadas con la violencia, representaron aproximadamente 37.5 billones JMD, o 160% del total de gastos de salud y el 4% del producto interno bruto de Jamaica. CONCLUSIONES: Disponer de datos confiables y exactos de la más alta calidad provenientes de los sistemas de información relacionados con la salud, resulta crucial a las hora de suministrar datos útiles sobre la carga de la violencia y las heridas para quienes tienen a su cargo las tomas de decisiones. Como que los Ministros de Salud desempeñan un papel dirigente en la prevención de las heridas y la violencia, la recogida de datos y los sistemas de información tienen que jugar un papel central. Este estudio describe los resultados de un enfoque para examinar la carga económica de la violencia interpersonal en los países en vías de desarrollo, en los cuales la carga de la violencia es más pesada. El manual CDC-OMS también probado en Tailandia y Brasil, es un primer paso hacia la generación de un punto de referencia para asignar recursos, establecer prioridades y defender la prevención.


Assuntos
Adolescente , Adulto , Feminino , Humanos , Masculino , Adulto Jovem , Efeitos Psicossociais da Doença , Custos de Cuidados de Saúde , Hospitalização/economia , Violência/economia , Ferimentos e Lesões/economia , Distribuição por Idade , Hospitalização/estatística & dados numéricos , Jamaica/epidemiologia , Prevalência , Distribuição por Sexo , Violência/estatística & dados numéricos , Ferimentos e Lesões/epidemiologia
10.
Mem. Inst. Oswaldo Cruz ; 104(1): 112-117, Feb. 2009. mapas, tab
Artigo em Inglês | LILACS | ID: lil-507215

RESUMO

Parasites are increasingly used to complement the evolutionary and ecological adaptation history of their hosts. Pneumocystis pathogenic fungi, which are transmitted from host-to-host via an airborne route, have been shown to constitute genuine host markers of evolution. These parasites can also provide valuable information about their host ecology. Here, we suggest that parasites can be used as phylogeographic markers to understand the geographical distribution of intra-specific host genetic variants. To test our hypothesis, we characterised Pneumocystis isolates from wild bats living in different areas. Bats comprise a wide variety of species; some of them are able to migrate. Thus, bat chorology and migration behaviour can be approached using Pneumocystis as phylogeographic markers. In the present work, we find that the genetic polymorphisms of bat-derived Pneumocystis are structured by host chorology. Therefore, Pneumocystis intra-specific genetic diversity may constitute a useful and relevant phylogeographic tool.


Assuntos
Animais , Quirópteros/microbiologia , Variação Genética , Geografia , Pneumocystis/genética , Argentina , Quirópteros/classificação , França , Guiana Francesa , México , Filogenia , Pneumocystis/classificação , Pneumocystis/isolamento & purificação , Análise de Sequência de DNA , Especificidade da Espécie
11.
Health SA Gesondheid (Print) ; 12(2): 27-36, 2007.
Artigo em Inglês | AIM | ID: biblio-1262390

RESUMO

This study describes the prevalence of virginity testing (VT) amongst rural secondary school students in KwaZulu- Natal (KZN); compares the attitudes of students of both sexes to VT; the differences in attitudes between girls who would/would not undergo such testing; and explores the relationship between risky sexual behaviour and girls who underwent virginity testing. A cross sectional descriptive study was undertaken with stratified random sampling of 10 secondary schools in Ugu District; KZN. Of 846 isiZulu-speaking students whose mean age was 16.1 years (SD 2.4); 492 (58.2) were girls; of whom 286 (58.1) had undergone VT and; in total 347 (70.5 ) girls supported VT.Girls whose mothers had less formal education were more likely to have participated in VT (P-0.03) with fewer older girls participating (P=0.0003). More girls than boys considered VT to protect against sexually transmitted infections (STIs) (P=0.02); and to be empowering (P0.005); but VT received support from both sexes as a traditional cultural practice. Participation in VT failed to prevent sexual intercourse and sexually transmitted diseases or to improve rates of condom use amongst those who were sexually active. South Africa's Bill of Rights supports gender equity. At community level VT has support despite its conflicting relationship with human rights; and questionable impact on preventing HIV/AIDS


Assuntos
Atitude , Percepção , Instituições Acadêmicas , Abstinência Sexual , Estudantes
12.
Artigo em Inglês | AIM | ID: biblio-1263442

RESUMO

Objective: This paper focuses on the use of participatory approaches to research and intervention and explores the uses of photo-voice as a methodology and a means of promoting childhood and youth activism in the context of HIV and AIDS.Method: Photo-voice; a visual participatory methodology; was used with 21 Grade 8 and 9 learners in one of three schools participating in a larger project; to identify; understand and interpret incidents related to stigma and discrimination against people living with and affected by HIV and AIDS; as well as the strategies proposed by the learners to possibly reduce stigma. Results: Three themes emerged: awareness of HIV and AIDS; awareness of HIV-related stigma and its impacts; and acceptance of personal agency and taking action. Conclusions: Understandings of and perceptions about HIV and AIDS are improving; yet significant pockets of ignorance about the dynamics of HIV infection still remain among the youth. Negative attitudes towards people infected with and affected by the virus remain; and stigmatisation continues. The use of photo-voice and other participatory methodologies offers alternative strategies for involving youth in their own knowledge production; as well as in the development of an individual sense of agency for taking action


Assuntos
Discriminação Psicológica , Infecções por HIV , Instituições Acadêmicas , Estigma Social
13.
Artigo em Inglês | AIM | ID: biblio-1263444

RESUMO

Objective:This paper focuses on the use of participatory approaches to research and intervention and explores the uses of photo-voice as a methodology and a means of promoting childhood and youth activism in the context of HIV and AIDS.Method: Photo-voice; a visual participatory methodology; was used with 21 Grade 8 and 9 learners in one of three schools participating in a larger project; to identify; understand and interpret incidents related to stigma and discrimination against people living with and affected by HIV and AIDS; as well as the strategies proposed by the learners to possibly reduce stigma. Results: Three themes emerged: awareness of HIV and AIDS; awareness of HIV-related stigma and its impacts; and acceptance of personal agency and taking action. Conclusions: Understandings of and perceptions about HIV and AIDS are improving; yet significant pockets of ignorance about the dynamics of HIV infection still remain among the youth. Negative attitudes towards people infected with and affected by the virus remain; and stigmatisation continues. The use of photo-voice and other participatory methodologies offers alternative strategies for involving youth in their own knowledge production; as well as in the development of an individual sense of agency for taking action


Assuntos
Discriminação Psicológica , Infecções por HIV , Instituições Acadêmicas , Estigma Social
14.
Artigo em Inglês | AIM | ID: biblio-1263453

RESUMO

Objective: This paper focuses on the use of participatory approaches to research and intervention and explores the uses of photo-voice as a methodology and a means of promoting childhood and youth activism in the context of HIV and AIDS. Method: Photo-voice; a visual participatory methodology; was used with 21 Grade 8 and 9 learners in one of three schools participating in a larger project; to identify; understand and interpret incidents related to stigma and discrimination against people living with and affected by HIV and AIDS; as well as the strategies proposed by the learners to possibly reduce stigma. Results: Three themes emerged: awareness of HIV and AIDS; awareness of HIV-related stigma and its impacts; and acceptance of personal agency and taking action. Conclusions: Understandings of and perceptions about HIV and AIDS are improving; yet significant pockets of ignorance about the dynamics of HIV infection still remain among the youth. Negative attitudes towards people infected with and affected by the virus remain; and stigmatisation continues. The use of photo-voice and other participatory methodologies offers alternative strategies for involving youth in their own knowledge production; as well as in the development of an individual sense of agency for taking action


Assuntos
HIV , Síndrome da Imunodeficiência Adquirida , Participação da Comunidade , População Rural , Instituições Acadêmicas , Estereotipagem
15.
Rev. argent. microbiol ; 37(1): 46-56, ene.-mar. 2005. ilus, tab
Artigo em Espanhol | LILACS | ID: lil-634488

RESUMO

Se comunica el primer aislamiento de Histoplasma capsulatum var. capsulatum de un murciélago macho de la especie Eumops bonariensis, capturado en la ciudad de Buenos Aires en 2003. Los aislamientos fueron recuperados de bazo e hígado e identificados fenotípicamente. Se los comparó por PCR, con 17 aislamientos clínicos, 12 de pacientes residentes en la ciudad de Buenos Aires y cinco de otros países de América, usando los iniciadores 1283, (GTG)5, (GACA)4 y M13. Con los cuatro iniciadores, los perfiles de los aislamientos de murciélago resultaron idénticos entre sí y más relacionados a los de pacientes de Buenos Aires que a los de otros países (porcentaje de similitud: 91-100% y 55-87%, respectivamente). La alta relación genética entre los aislamientos obtenidos del murciélago y de los humanos residentes en Buenos Aires sugiere una fuente común de infección. Este es el primer registro de E. bonariensis infectado con H. capsulatum en el mundo, y el primer aislamiento del hongo en la población de quirópteros de la Argentina. Así como estos mamíferos actúan como reservorio y dispersan el hongo en la naturaleza, la infección en murciélagos urbanos podría asociarse al elevado número de casos de histoplasmosis entre pacientes inmunodeprimidos en la ciudad de Buenos Aires.


We report the first isolation of Histoplasma capsulatum var. capsulatum from a male bat Eumops bonariensis captured in Buenos Aires city in 2003. The pathogen was recovered from spleen and liver specimens, and was identified by its phenotypic characteristics. PCR with primers 1283, (GTG)5, (GACA)4 and M13 was used to compare both bat isolates with 17 human isolates, 12 from patients residing in Buenos Aires city, and 5 from other countries of the Americas. The profiles obtained with the four primers showed that both bat isolates were identical to each other and closer to Buenos Aires patients than to the other isolates (similarity percentage: 91-100% and 55-97%, respectively). The high genetic relationship between bat isolates and those from patients living in Buenos Aires suggests a common source of infection. This is the first record of E. bonariensis infected with H. capsulatum in the world, and the first isolation of the fungus in the Argentinean Chiroptera population. In the same way as these wild mammals act as reservoir and spread the fungus in the natural environment, infection in urban bats could well be associated with the increase in histoplasmosis clinical cases among immunosuppressed hosts in Buenos Aires city.


Assuntos
Animais , Humanos , Masculino , Quirópteros/microbiologia , Histoplasma/isolamento & purificação , América , Argentina/epidemiologia , Quirópteros/classificação , Reservatórios de Doenças , DNA Fúngico/genética , Histoplasma/genética , Histoplasmose/epidemiologia , Histoplasmose/microbiologia , Histoplasmose/transmissão , Hospedeiro Imunocomprometido , Fígado/microbiologia , Infecções Oportunistas/epidemiologia , Infecções Oportunistas/microbiologia , Infecções Oportunistas/transmissão , Especificidade da Espécie , Baço/microbiologia , Saúde da População Urbana
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