RESUMO
Introduction: The field of bio-safety covers risk assessment, management of such risks, the regulation, communication & mitigation of adverse events with the aim of promoting a safe environment for Clinical Laboratory testing. Methodology: This was a comparative cross sectional study which used a standardized, structured self-administered questionnaire consisting of thirty question, ten each to survey Knowledge, Attitude and Practice(KAP) of bio-safety among healthcare workers(HCWs). A total of 75 randomly selected HCWs participated in the study. Results: Awareness regarding Universal precaution guidelines and hand hygiene were found to be 98.67% and 88% respectively. 80% HCW know highest risk of acquiring Hepatitis B infection after Needle Stick Injury(NSI), 92% know it is prevented by vaccination. However only 74.67% know what to do in case of NSI, only 25.33% know to write injury report to authorities. Though 45.33% participants had NSI, only 6.66% reported to authority. 73.33% were aware about Bio-medical Waste(BMW) rules 2016. Only 52% participants were aware that segregation is the key step in BMW management and the knowledge improved significantly after training.