RESUMO
The shortened duration of the winter season in the Himalayan region caused by snow melting has a negative impact on fruit crop growth and productivity. The present study focused on examining the impact of climate change on fruit crops in the Solan district of Himachal Pradesh, India, situated in the Himalayan region. The trend analysis of climatic variables (temperature and rainfall) along with the productivity of fruit crops was investigated. The climate data spanning 30 years (1990–2019), including average temperature (maximum, minimum, and diurnal) and annual rainfall used during crop development stages like pre-flowering, flowering, and fruit-setting stages. To evaluate climatic trends, the Standardized Anomaly Index (SAI) and Mann-Kendall Test for quantification were employed. The Multivariate Linear Regression Analysis was performed to establish a correlation between climatic variables and crop productivity. The findings indicated that during the pre-flowering stage, there was a gradual increase in average maximum temperature at a rate of 0.001°C per year, along with a corresponding rise in diurnal temperature at a rate of 0.036°C per year. However, annual rainfall and average minimum temperature exhibited non-significant decreasing trends, with rates of -0.044°C and -0.033 mm, respectively. During the flowering stage, there was a significant increase in minimum temperature at a rate of 0.151°C per year, while diurnal temperature exhibited a significant decrease of -0.158°C per year. Other variables did not exhibit substantial changes during this stage. In the fruit-setting stage, only the minimum temperature demonstrated a significant decrease over the study period. The response to climate change revealed an overall positive trend for all fruit crops, leading to higher productivity. The correlation study indicated that the phenological stages of each crop were more positively influenced by temperature than rainfall, owing to existing climatic variations. The current climatic conditions in the Solan district were found to be favorable and productive for crop development, as all crops showed increased productivity based on the trend analysis. The study highlights climatic trends and their impact on the productivity of fruit crops in the Himalayan region, which is useful for agricultural planning and adaptation strategies in response to changing climatic conditions.
RESUMO
A drug-like molecule, which has a propensity of binding with DNA play a vital role in drug designing mechanism. In this paper, we tried to find out the DNA binding affinity of two derivatives of N-arylhydroxamic acids: (i) N-p-Chlorophenyl-2-methoxybenzohydroxamic Acid(Cl-2-MBHA) and (ii) N-p-Chlorophenyl-3-methoxybenzohydroxamic Acid (Cl-3-MBHA) with calf thymus DNA (ct-DNA) by applying techniques such as UV-visible spectroscopy, Fluorescence spectroscopy, and Viscometry measurements. The findings concluded with experimental techniques were verified with theoretical calculation using computer-based method, Molecular Docking. Absorption spectra revealed that both the hydroxamic acids derivatives bind to ct-DNA, among two, Cl-2-MBHA exhibits the higher value of binding affinity Kb (9.52 × 103±0.08 M-1). Fluorescence spectra showed that ct-DNA successfully quenches the emission spectra of N-arylhydroxamic acid. Ethidium bromide displacement method was used as a standard for analyzing the mode of binding. Both the hydroxamic acids were found to be groove binders. The Stern–Volmer Constant was found to be 2.05 × 10-2 ± 0.001 M-1 and 3.35 × 10-2 ± 0.002 M-1 for Cl-2-MBHA and Cl-3-MBHA respectively. Theoretical analysis molecular docking was done using Hex software for validating the experimental findings. Hence, it was observed that both experimental and computational method complimented the results and deduces groove binding as the mode of interaction.