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ObjectiveTo observe that effect of Ersi decoction on rats with rheumatoid arthritis （RA） induced by using the complete Freund's adjuvant emulsion containing bovine type Ⅱ collagenand and elucidate underlying menchanisms involving to inhibit inflammation and joint synovial angiogenesis. MethodThe rat model of RA was established by immune induction with complete Freund's adjuvant emulsion containing bovine type Ⅱ collagen. All male SD rats were randomly divided into blank group， RA model group， methotrexate group（1.0 mg·kg-1）， and low-， medium- and high-dose group（30，15，7.0 g·kg-1·d-1）of Ersi decoction， with 8 rats in each group. Except the blank group， rats in the methotrexate group and Ersi decoction groups were given corresponding doses of methotrexate and Ersi decoction after establishment of RA induced by strengthen immunity，respectively，and those in the model group and blank group received normal saline of equivalent volume，once a day for 28 days. After the administration， the degree of joint swelling of rats in each group was analyzed by joint swelling volume and index. The small animal ultrasound imaging system was used to detect the score and area of synovial hyperplasia of knee joint in right lower limb of rats and hematoxylin-eosin（HE）staining to observe the histomorphological changes in joint synovium of rats. The levels of tumor necrosis factor-α（TNF-α） and interleukin-1β（IL-1β） were measured by enzyme-linked immunosorbent assay（ELISA）. Immunohistochemistry was employed to analyze the expression of CD31 and vascular endothelial growth factor receptor 2（VEGFR2） in in joint synovium. ResultCompared with the blank group， the model group demonstrated significant increase in joint swelling volume and index， inflammatory cytokines including TNF-α and IL-1β in serum， the score and area of synovial hyperplasia of knee joint in right lower limb， obvious pathological changes in the synovium and the expression of CD31 and VEGFR2 in joint synovium. Medium and high-dose Ersi decoction significantly alleviated the pathological changes of synovium tissue， attenuated joint swelling volume and index and decreased the expression of CD31 and VEGFR2 in joint synovium as compared with the model group. Moreover， high-dose Ersi decoction showed significantly lower levels of TNF-α and IL-1β in serum， and the score and area of synovial hyperplasia of knee joint in right lower limb. But medium-dose Ersi decoction only showed lower levels of TNF-α and area of synovial hyperplasia of knee joint. ConclusionErsi decoction could reduce synovial inflammation and hyperplasia through inhibiting synovial angiogenesis in rats with RA induced by bovine type Ⅱ collagen for achieving the effect of reducing RA joint damage， which provides an important reference for anti-RA of Ersi decoction in clinical application.
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<p><b>OBJECTIVE</b>To compare the influences of gold alloy metal crown and Ni-Cr alloy metal crown on gingival health.</p><p><b>METHODS</b>Totally 20 patients requiring one metal crown restoration were divided into the gold alloy metal crown group (n=9) and Ni-Cr alloy metal crown group (n=11). The contra-lateral homonymy natural healthy teeth served as controls. Before the tooth preparation and 6 months after crown placement, the weight of gingival crevicular fluid (GCF) of each tooth (included the test teeth and the control teeth) was measured, and the probing depth and the sulcus bleeding index of each tooth were also recorded.</p><p><b>RESULTS</b>In the gold alloy metal crown group, the weight of GCF detected before the tooth preparation was significantly larger than that detected 6 months after restoration (P<0.05). In the Ni-Cr alloy metal crown group, the sulcus bleeding index recorded 6 months after restoration was significantly larger than that recorded before the tooth preparation (P<0.05). The other experimental indicators were not significantly different before and after restoration.</p><p><b>CONCLUSION</b>The gold alloy metal crowns will not cause obvious harm to the periodontal tissues of the abutments shortly after restoration, while the Ni-Cr alloy metal crowns may increase the risk of sulcus bleeding.</p>