Downregulation of cellular prion protein inhibited the proliferation and invasion and induced apoptosis of Marek's disease virus-transformed avian T cells

Cellular prion protein (PrP(C)) is ubiquitously expressed in the cytomembrane of a considerable number of eukaryotic cells. Although several studies have investigated the functions of PrP(C) in cell proliferation, cell apoptosis, and tumorigenesis of mammals, the correlated functions of chicken PrP(C) (chPrP(C)) remain unknown. In this study, stable chPrP(C)-downregulated Marek's disease (MD) virus-transformed avian T cells (MSB1-SiRNA-3) were established by introducing short interfering RNA (SiRNA) targeting chicken prion protein genes. We found that downregulation of chPrP(C) inhibits proliferation, invasion, and migration, and induces G1 cell cycle phase arrest and apoptosis of MSB1-SiRNA-3 cells compared with Marek's disease virus-transformed avian T cells (MSB1) and negative control cells. To the best of our knowledge, the present study provides the first evidence supporting the positive correlation between the expression level of chPrP(C) and the proliferation, migration, and invasion ability of MSB1 cells, but appears to protect MSB1 cells from apoptosis, which suggests it functions in the formation and development of MD tumors. This evidence may contribute to future research into the specific molecular mechanisms of chPrP(C) in the formation and development of MD tumors.

Analysis of anxiety influencing factors of computer department students in vocation selecting period / 中华行为医学与脑科学杂志

Objective To explore the anxiety influencing factors of students of computer department in vocation selecting period.Methods A total of 363 students of computer department in periods of vocational selection were assessed with 16PF, SCL-90, Coping Style Questionnaire,and Vocational Selection Anxiety Questionnaire for University Graduate. Results The anxiety score of students of computer department in vocation selecting period were much higher statisticaly than the Chinese youth norm(t=6.631,P=0.000).The results of multivariable stepwise regression showed that the major factors affecting the anxiety score of the students were anxiousness (stb=0.24928,t=4.72,P＜0.000),press of employment compete(stb=0.22126,t=4.54,P＜0.000),apprehension(stb=0.18320,t=3.36, P=0.001),vigilance (stb=0.15002,t=3.16, P=0.002), family-incompleteness (stb=0.09474,t=2.17, P=0.031)et al. Conclusion The anxiety score were affected by many factors. Unhealthy personality such as high vigilance and high anxiousness are the internal factors of the vocation selecting anxiety, press of employment compete is only the inducing factor. Family-incompleteness has adverse effect on response ability of the students.