Role of combination of autophagy inhibition and anti-VEGF in angiogenesis of retinal vascular endothelial cells / 实用医学杂志

Objective In this study,we explored the role of combination of autophagy inhibition and anti-VEGF in proliferation,migration and tube formation of mouse retinal vascular endothelial cells(RVECs). Methods Well cultured mouse RVECs were randomly divided into four groups:autophagy inhibition group(add-ing autophagy inhibitor 3-MA),anti-VEGF group(adding anti-VEGF-A neutralized antibody),autophagy inhibi-tion+anti-VEGF group(adding the two reagents)and the control group.All cells were then cultured in the hypoxic condition. The cell proliferation,migration and tube formation were detected by EdU,transwell and matrigel as-say,respectively. Results The cell proliferation rate,number of migrated cells and number of tube formation of the other three groups decreased when compared with the control group.These data above in autophagy inhibition+anti-VEGF group were all significantly less than 3-MA group and anti-VEGF group. Conclusion Combination of autophagy inhibition and anti-VEGF may be more effective than simple anti-VEGF in inhibition of retinal neovascu-larization.

Clinical observation of blood serum bilirubin level and the degree of coronary artery narrowing / 西安交通大学学报(医学版)

Objective To observe the relationship of blood serum bilirubin level with coronary heart disease and the degree of coronary artery narrowing. Methods A total of 126 patients were divided into the coronary disease group (83) and the control group (43). According to the coronary artery narrowing integral, the 83 patients in the coronary disease group were divided into three subgroups: mild narrowing group (15 people), moderate narrowing group (35 people), and severe narrowing group (33 people). The coronary arteriography of the patients in the control group was normal. 5mL venous blood was drawn on empty stomach, and the enzyme method was used to determine glycerin, the total cholesterol, the low density lipoprotein cholesterol, the high density lipoprotein cholesterol, and the blood serum bilirubin. Results The blood serum total bilirubin in the coronary disease group was (12.30?3.84)?mol/L, direct bilirubin was (4.07?1.45)?mol/L, and indirect bilirubin was (8.23 ? 2.82 )?mol/L. The total bilirubin in the normal group was (14.59?4.37)?mol/L, direct bilirubin was (4.66 ? 1.55 )?mol/L, and indirect bilirubin was (9.93?3.33)?mol/L. The total bilirubin, indirect bilirubin and direct bilirubin were lower in coronary disease group than in normal group (P0.05). Conclusion The blood serum bilirubin level and coronary disease have remarkable negative correlation, and the blood serum bilirubin level of patients with coronary disease is lower than that of healthy people. ② The blood serum bilirubin level and the degree of coronary artery narrowing do not have remarkable correlation.