PCR OF THE MAIN REPETITION SEQUENCE OF THE INAZ GENE IN THE DNA OF INA~- AND INA~+ BACTERIA AND FUNGI / 微生物学通报

The oligonucleotide primer having the 5’\|GCCGGTTATGGC AGCACGCTGACC\|3’ was synthesized,which encodes a main repetition motif of protein produced by the inaZ gene.PCR of the primer in the DNA of INA - and INA + bacteria and fungi was done.All of the DNA of INA - and INA + bacteria and fungi expressed bands,the results show that the primer did not work for distinguishing the INA - and INA + organisms and for identification of the ice nucleation activity of the INA organisms by the size and brightness of the PCR products.