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Objective To conduct the molecular epidemiologic analysis of Staphylococcus aureus (S.aureus) in the intensive care units(ICUs) and general wards and to compare their clinical characteristics.Methods Ninety-six clinically isolated strains of S.aureus(43 strains from the emergency intensive care unit(EICU) and neurosurgical intensive care unit(NICU) and 53 strains from the general wards) collected from Sepetember 2015 to April 2016 were performed the bacterial identification and antibiotic susceptibility test.The molecular typing was performed by adopting staphylococcal protein A (spa) typing method.Results Among 96 strains of S.aureus,the detection rate of methicillin-resistant S.aureus(MRSA) was 40.6%(39/96),which among 43 strains in ICU was 62.8%(27/43) and which among 53 strains in the general words was 22.6%(12/53).The resistance rates of strains from ICUs to gentamicin,levofloxacin,clindamycin,fosfomycin and minocycline were 23.3%,48.8%,46.5%,32.6% and 32.5% respectively,while which from the general wards were 7.5%,24.5%,18.9%,2.1% and 0% respectively.The Spa typing results showed that the main types of ICUs were t002,t091 and t311.The major epidemic strain was t002(n=16,37.2%) and mainly isolated from EICUs(12 strains),26 spa types were identified among the general wards trains,mainly were t189,t377,t571,t034,t091,t127.Conclusion The detection rate of MRSA in ICUs is higher than that in the general wards,these strains have high resistant rate to routine antibacterial drugs.t002 is the major epidemic strain.The general wards have more spa types with higher genetic diversity.
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OBJECTIVE To monitor and analyze the distribution and antibiotic resistance of common bacteria isolated from urine-tract infection patients in our hospital in recent two years and to provide new evidence for clinicians to choose the antibiotics. METHODS We used VITEK system to identify the bacteria,performed the drug-resistant test by K-B and analyzed the distribution and drug resistance of the isolated bacteria by WHONET 5.3 software. RESULTS A tatal of 1379 strains of bacteria were isolated from 2991 urine samples,most of which were Gram-negative bacteria.among which Escherichia coli was the most common.Enterococcus were the most common pathogen of gram-positive bacteria,especially for E.faecalis and E.faecium and fungi infection also increased.The resistance to antibiotics was quite different,manifesting multi-resistance.The detection rates of ESBLs-producing E.Coli,Klebsiella penumoniae and Proteus were 72.1%,69.9% and 34.2%,respectively. CONCLUSIONS It′s of great significance to summarize and analyze the distribution and biotic resistance of isolated bacteria in urine-tract infection to control resistant strains and indicate the choice of antibiotics in clinic.
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Objective:To study the detecting rate of nonfermenters and their resistance to the antibiotics in nosocomial infections in our hospital. Methods:The bacteria were identified by VITEK-AMS, the susceptibility tests(K-B method) were done according to the NCCLS standard and the results were analyzed. Results: The detecting rate of nonfermenters was 14. 29%. The most common bacterium was P. aeruginosa (the component ratio was 44. 22%). followed by A. baumannnii (32. 17%) and S.maltophilia(9.23%). The infectious rates of nonfermenters were different at different infectious sites, with the highest rate in the respiratory system and the lowest in central nervous system. The resistant rate to the antibiotics of P. aeruginosa was 45. 21% ,of A. baumannnii 47. 85% and of S. maltophilia 64. 02%. Conclusion:The detecting rate of nonfermenters is rather high in the nosocomial infection in our hospital. Due to the high resistant rates of nonfermenters to the antibiotics , the drugs should be chosen accoding to the result of the drugs susceptibility test.