RESUMO
Background: Many biomarkers have now been studied such as C-reactive Protein (CRP), procalcitonin (PCT), etc., and are widely used for the diagnosis of sepsis in clinical practice which may determine the appropriate antibiotic treatment. A flowcytometric cytokine bead array (CBA) assay has now been used to determine multiple interleukins (IL), simultaneously. The aim of this study was to determine the cytokine (IL2, IL4, IL6, IL10, TNF?, INF?, and IL17) profiles of interleukins in plasma of sepsis patients by using multiplex Flowcytometric CBA array assay. Materials and Methods: A total of 99 consecutive patients admitted with the suspected sepsis were studied. PCT concentrations were measured by using the enzyme-linked fluorescent immunoassay (ELFA) technique and flow cytometry-based BD™ CBA Cytokine Kit was used to evaluate levels of 7 cytokines [IL-2, IL-4, IL-6, IL-10, Tumour Necrosis Factor (TNF), Interferon- ? (IFN-?), and IL-17A]. Results: Microbiologically defined infection (MDI) demonstrated a positive culture report in 79/99 (79.7%) of patients. The IL6 [1873.7 (4-5000)] and IL10 [(154.7 (0-1764)] levels were significantly higher in septic patients than those in the negative MDI IL6 [901 (4-5000)] and IL10 [110.4 (4-1372)] levels. The AUROC value of IL6 [0.66 (0.53-0.79)] was found to be the highest among all followed by IL10 [0.65 (0.51-0.79)], IFN? [0.63 (0.51-0.77)], PCT [0.61 (0.48-0.75)], and TNF? [0.55 (0.42-0.69)]. Conclusion: Our study suggests that that IL6 is substantially more economical and can reduce the investigation cost to half as compared with the procalcitonin assay.
RESUMO
Objective: Malnutrition is a critical public health concern in India. We aimed to understand the prevalence of stunting and its association between cooking fuel type and breastfeeding among Indian infants (? 6 months of age). Methods: This study used information from a National Family Health Survey-4 (NFHS) conducted between 2015 and 2016. From all eligible infants, information on socio-demographic and clinical characteristics was obtained. Univariate and multivariable binary logistic regression was performed to examine the factors associated with stunted children. Results: Of the 15,120 infants, the median (Inter Quartile Range) age was 4 (2-5) months with a female/male ratio of 1:1.06. The prevalence of polluting fuel use and stunting was 68.10% and 20.13% respectively. Stunting among polluting fuel and clean fuel was 22.17% and 15.78%. Stunting among exclusive breastfed and not exclusive breastfed was 20.62% and 19.53%. In adjusted analysis, households using clean fuel (Adjusted Odds Ratio 0.75; 95% Confidence Interval, 0.66-0.86) had lower odds of infants being stunted compared to polluting fuel. A infant currently being breastfed (aOR 0.76; 95% CI, 0.62-0.93) had lower odds of being stunted than infants currently not being breastfed. Conclusion: The present study indicates that polluting cooking fuel and not being breastfed was associated with an increased risk of infant being stunted. A community-based randomized control trial was required to study child and maternal health risks on polluting cooking fuel. Furthermore, the study highlights the urgent need to raise awareness on the importance of exclusive breastfeeding practices.