RESUMO
ObjectiveTo investigate whether the reactive oxygen species involved in inhibiting proliferation of human bone marrow cells of neuroblastoma cells (SH-SY5Y)process by giant typhonium rhizome water extract,and to investigate the initial mechanism of anti-tumor effect of giant typhonium rhizome in vitro.MethodsAfter giant typhonium rhizome water extract processing of human bone marrow neuroblastoma cells (SH-SY5Y)at 24 h and 48 h,the cells were collected,and western blotting reaction methods such as flow cytometry was used to study the levels of ROS and SH-SY5Y apoptosis.ResultsThe levels of ROS elevated from before dosing (4.9±1.6)％ to (39.7 ± 11.8)％ in the process of giant typhonium rhizome water extract induced apoptosis in SH-SY5Y; after ROS inhibition was added,SH-SY5Y apoptosis level induced by giant typhonium rhizome water extract had decreased.ConclusionROS involved in the process of giant typhonium rhizome water extract inhibiting cell proliferation in SH-SY5Y.
RESUMO
ObjectiveTo investigate the anti tumor effect of Giant Typhonium Rhizome.Methods SH-SY5Y was treated for 24 or 48 h by Giant Typhonium Rhizome.Cell Counting Kit-8 (CCK-8) cell proliferation and cytotoxicity assays were applied to detect the inhibition of SH-SY5Y cells treated with aqueous extract of white monkshood root.AV/PI double staining flow cytometry was applied to explore the mechanism of growth inhibition.ResultsSH-SY5Y cells could be inhibited by root aqueous extract of Giant Typhonium Rhizome,the IC50 at 48 h (50％ inhibitory concentration) was 0.862 mg/ml,while the IC50 at 24 h was 1.158 mg/ml.Western-blot results showed that root aqueous extract of Giant Typhonium Rhizome could induce apoptosis to inhibit tumor cells.ConclusionThe Rhizoma typhonii inhibit the growth of SH-SY5Y.