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1.
Rev. cuba. med. trop ; 55(2)mayo.-ago. 2003. tab, graf
Artigo em Espanhol | LILACS | ID: lil-386992

RESUMO

Se empleó la técnica de neutralización por reducción del número de placas (NRNP) para la detección de anticuerpos antirrábicos en personal de riesgo. Se estudiaron muestras de suero de individuos de alto riesgo y personas con antecedentes de vacunación y sin estos. La técnica de neutralización fue comparada con la prueba biológica en ratón y como resultado del estudio se obtuvo una concordancia de 100 por ciento. Se encontró que el sexo no influye en la respuesta de anticuerpos a este virus, aunque el contacto directo y la cantidad de veces que el individuo ha recibido la vacuna actúan positivamente en la respuesta inmune. La introducción de esta técnica en el laboratorio permite contar con una herramienta útil, en el seguimiento del personal que recibe vacunación preexposición y posexposición al virus rábico


Assuntos
Humanos , Animais , Camundongos , Raiva , Testes Sorológicos , Vacina Antirrábica/imunologia , Testes de Neutralização/métodos
2.
Rev. cuba. med. trop ; 52(3): 211-214, Sept.-Dec. 2000.
Artigo em Espanhol | LILACS | ID: lil-333469

RESUMO

225 monosera from patients (121 adults and 104 children) with different malignant haemopathies from the Institute of Hematology were studied. The hemagglutination inhibition (HI) technique and the neutralization technique were used to know the presence of antimeasles antibodies. All these patients were under immunosuppression treatment at the time of taking the specimen and children had received the triple viral vaccine (measles, rubella, parotiditis) before the disease was diagnosed. It was found that 51 (42.14) of the adults' specimens were positive by HI and of the 25 negative specimens that underwent the neutralization technique, 18 (72) proved to be positive. Of the 104 sera corresponding to children, 31 (29.8) specimens were positive by HI, whereas 29 of the negative were studied by neutralization. 18.34 of all the specimens continued to be negative by both methods. According with the 2 techniques used, the presence of measles virus antibodies was of 81.66 in children and of 90.78 in adults.


Assuntos
Humanos , Criança , Adulto , Anticorpos Antivirais , Leucemia , Linfoma , Vírus do Sarampo/imunologia
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