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1.
Southeast Asian J Trop Med Public Health ; 1999 Mar; 30(1): 161-5
Artigo em Inglês | IMSEAR | ID: sea-35812

RESUMO

A virus isolate, ThCAr105/92, from a pool of mosquitos, Culex tritaeniorhynchus, collected in Chiang Mai, Thailand in 1992, appeared to be a member of the genus Flavivirus of the family Flaviviridae, based on the reverse transcription polymerase chain reaction (RT-PCR) using flavivirus cross-reacting primer pairs, electron microscopic examination, and serological tests. However, RT-PCR using Japanese encephalitis (JE) virus-specific primers showed that the isolate was different from JE virus. Sucrose density gradient sedimentation of the virus replicated in C6/36 cells indicated that the virus is relatively unstable in the infected culture fluids at 37 degrees C. Antibody prepared against this virus and a virus seed for the isolate were tested by cross neutralization against a panel of flaviviruses and the results showed that the new isolate was a distinct subtype of Tembusu virus.


Assuntos
Animais , DNA Viral/análise , Flavivirus/classificação , Microscopia Eletrônica , Testes de Neutralização , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sorotipagem , Tailândia , Virologia , Cultura de Vírus
2.
Southeast Asian J Trop Med Public Health ; 1996 Dec; 27(4): 818-27
Artigo em Inglês | IMSEAR | ID: sea-33858

RESUMO

Electrophoretic studies were carried out on isozymes of 3 populations of Anopheles minimus collected from Guangxi and Yunnan Provinces of the People's Republic of China in 1993. Eight proteins were analyzed by 5% polyacrylamide gel electrophoresis. The most variable population, Y-F, was highly polymorphic at 14 of 20 loci (P=0.700) with an average heterozygosity H of 0.340. P values of 0.500 and 0.700, and H values of 0.220 and 0.210 were obtained for each from 'Guangxi-Lab' (GX-L) and 'Yunnan-Lab' (Y-L), respectively. Nei's genetic distances (D) between Y-L and GX-L, Y-F and GX-L, and Y-F and Y-L were 0.1131, 0.1946 and 0.1069, respectively. These results suggest that GX-L is distant from the 2 other populations, Y-L and Y-F, and that this genetic differentiation between the 2 populations of Yunnan and Guangxi Provinces corresponds to the forms A and B, which were morphologically classified by Xu et al (unpublished).


Assuntos
Alelos , Animais , Anopheles/genética , China , Eletroforese em Gel de Poliacrilamida , Variação Genética , Proteínas de Insetos/genética
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