A novel HPTLC method for quantitative estimation of biomarkers in polyherbal formulation

Objective:To explore the quantitative estimation of biomarkers gallic acid and berberine in polyherbal formulation Entoban syrup. Methods: High performance thin layer chromatography was performed to evaluate the presence of gallic acid and berberine employing toluene:ethyl acetate:formic acid:methanol 12:9:4:0.5 (v/v/v/v) and ethanol: water: formic acid 90:9:1 (v/v/v), as a mobile phase respectively. Results:The Rf values (0.58) for gallic acid and (0.76) for berberine in both sample and reference standard were found comparable under UV light at 273 nm and 366 nm respectively. The high performance thin layer chromatography method developed for quantization was simple, accurate and specific. Conclusions: The present standardization provides specific and accurate tool to develop qualifications for identity, transparency and reproducibility of biomarkers in Entoban syrup.

Xanthine oxidase inhibition by some medicinal plants.

Xanthine oxidase inhibitory activity has been reported from different plants such as Cinnamomum cassia, Chrysanthemum indicum, Lycopus europaeus, Polygonum cuspidatum, Acacia confuse, Coccinia grandis, Datura metel, Strychnos nux-vomica, Vitex negundo, Coccinia grandis, Vitex negundo, Fraxinus angustifolia, Pistacia lentiscus, Hyptis obtusiflora, H. lantanaefolia, Artemisia vulgaris, Caesalpinia sappan, Blumea balsamifera, Chrysanthemum sinense, Tetracera scandens, C. sinense, Allium Cepa, Pistacia integerrima, Caesalpinia sappan and Caesalpinia sappan. This review very clearly specify that plants could be utilized for the inhibition of xathine oxidase and out of them Clerodendrum floribundum, Eremophila maculata, Stemodia grossa Benth, Eucalyptus deglupta, Syzygium malaccense and Larix laricina exhibited 84%, 61%, 57%, 51%, 64%, 86 % xanthine pxidase inhibition at concentration of 50 μg/ml, 50 μg/ml, 50 μg/ml, 44.5 μg/ml, 51 μg/ml, 6.26mg/dl respectively.

Allium sativum Linn a review of its pharmacological attributes

Garlic, a member of the lily family, is a perennial plant that is cultivated worldwide. The garlic bulb is composed of individual cloves enclosed in a white sheath or skin. It is the bulb, either fresh or dehydrated, that is used as a spice or medicinal herb. The garlic oil is obtained by steam distillation of the crushed fresh bulbs