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Inflammatory bowel disease [IBD] is a chronic disease of unknown etiology which mostly involves the intestine and requires a personalized approach for treatment. IBD represents a heterogeneous group of patients with inherently variable disease courses. Hence, the heterogeneity of patient populations may delay the diagnosis, clinical practice and initiation of appropriate treatment. Use of biomarkers for diagnosis and management of IBD is still necessary. Descriptions of the immunological pathway abnormalities in IBD improve assessment to identify the patient's disease status, and relative risk of progression to complicated disease behaviors, and this information may ultimately influence therapeutic decisions. In this study, we try to explain the role of biomarkers in early diagnosis, estimating prognosis, and target agents for correct managements of IBD's patients. This information might be important to provide insight into emerging panels of multiple IBD biomarkers and highlighting the essential role of personalizes panel for each patient
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Aim: The aim of this study was to find the relationship between rs1799964 in TNF-alpha gene as well as rs1051208 of RAF1 gene SNPs on GC in an Iranian population
Background: Gastric cancer [GC] is the second leading cause of cancer-related death worldwide after lung cancer. Tumor necrosis factor [TNF] is one of the most important factors in the pathogenesis of this cancer. Single nucleotide polymorphisms have a principle role in gene expression of TNF-alpha and miRNAs which may lead to gastric cancer
Methods: In a case-control study, we investigated the risk of GC in 198 Iranians. For this purpose, 5 mL of peripheral blood was collected in EDTA -containing tube and genomic DNA was isolated. Genotyping of SNPs was also performed by PCR-RFLP; to approve the outcome, 10% of genotyping results with RFLP were sequenced
Results: The comparison between case and control groups revealed a significant association between the rs1051208 C allele of RAF1 gene and GC [P = 0.04]. We did not observe any remarkable association between TNF-alpha -1031 in gastric cancer patients and the healthy control group
Conclusion: The results indicated that C allele in RAF1 gene plays a role in susceptibility to gastric cancer. Therefore, SNPs are among notable biomarkers for predicting susceptibility to dreadful diseases, especially cancers
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This study aimed to determine the association between PD-1.5C/T [rs2227981, +7785] and the risk of gastric cancer [GC] in an Iranian population. Gastric cancer is the fourth most common cancer in the world. The programmed death 1 [PD-1] is a member of the CD28 super family. PD-1 is a negative regulator of T-cell effector mechanisms which decrease immune responses against cancer. we conducted case- control study to investigate the association of PD-1.5 C/T polymorphism in 122 GC patients and 166 control individuals. DNA was extracted from blood specimens. Genotypes were analyzed using polymerase chain reaction-restriction fragment length polymorphism [PCR-RFLP] assay. The frequency of CC, CT and TT genotypes was 53.6%, 42.2% and 4.2% in control group and 41%, 54.1% and 4.9% in gastric cancer patients respectively. CC genotype was more frequent in control individuals than in patients but we found no statically significant association. The frequencies of PD-1.5CT genotypes were significantly higher in GC patient compared with control individuals [OR= 1.77, 95% CI= 1.077-2.931; P=0.026]. Allele distribution was similar in patients and healthy individuals [p=0.061].Frequency of C and T alleles was 74.7%, 25.3% in control individuals and 68.03% and 31.97% in gastric cancer patients respectively. These results suggest that PD-1.5 C/T polymorphism may affect the GC risk and prognosis in an Iranian population
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The purpose of this study was to determine the relationship of rs4444903 [EGF+61A/G] SNP genotype with colorectal cancer and tumor stage in an Iranian population. Epidermal growth factor [EGF] is one of the important proteins that determine survival of cells. EGF binds to its receptor on the cell surface and then activates some of the cell signaling pathway networks within cells that lead to activation or deactivation of factors which are responsible for growth and apoptosis of cells. In this study we assessed the association in EGF polymorphism rs4444903 with colorectal cancer [CRC] in Iranian population. We conducted case-control study to investigate the association of polymorphism rs4444903 in EGF, with colorectal cancer risk in Iranian population. Analyzed Polymorphism of EGF rs4444903 with restriction fragment length polymorphisms [RFLP] among two groups of subjects consisting of including 220 cases with colorectal cancer and 220 healthy individuals as controls. Mutations were confirmed in 10% of the samples by direct sequencing. The frequencies of AA, AG and GG genotypes among cases with colorectal cancer were 28.2, 46.8, and 25.0% respectively and in controls genotype frequencies were 23.2, 56.4, and 25.0%, respectively. Frequency of A allele among case group was 51.6% and for control group was 51.4%. The frequency of G allele in case and control was, respectively 48.4% and 48.6% [OR= 1.009, 95% CI= 0.775-1.315; P= 0.946]. The percentage of Stage 0, I, II, III, IV were 5%, 9.35%, 38.84%, 30.21% and 16.54%, respectively, among the cases. However, no significant association between this polymorphism and CRC stage was observed [p=0.626]. Our data suggest a SNP rs4444903 may not represent a risk factor in the development and progression of CRC among Iranian population