Morphometric Evaluation of Mental Foramen of Dry Adult Human Mandibles of South Indian Population and Their Clinical Correlation

Introduction: Morphometric assessment of mental foramina (MF) of the mandible for its variable position is considered to be helpful for the surgeons and dentists for the localized surgical procedures and for the anesthetists to execute nerve block practices. Hence the objective of the present study is to establish the morphometric variability in the position of MF in terms of gender in the mandibles of Southern Indian origin with possible clinical implications. Methods: The shape, size, and location of MF were measured on both sides of the mandible (n=92). Location of MF was established by measuring the distance from MF to the [1] mental symphysis [2] alveolar crest, [3] the base of the mandible, and [4] posterior border of the ramus of the mandible. All the morphometric data were analyzed statistically with a significance level of p<0.05. Results: The higher incidence (58.7%) of rounded MF was recorded. There were no cases of MF anterior to the first pre-molar and below the 1st molar. Most of them (45.7%) were below the 2nd premolar. There was a significant association between gender and the position of the MF (p=0.011). But, no association between gender and side as well as the shape was noted. Significant associations were found between gender and the distance from mental foramen to the posterior border of the ramus and also to the alveolar crest. A significant association between the gender and the positions of the MF was noted. Conclusion: The variations observed from previous studies might be related to the feeding habits of different regions which may ultimately, affect the development of mandibles. Prior knowledge regarding the common positions of MF in local populations is helpful in effective nerve blocks and maxillofacial surgeries.

Retrospective Cross Sectional MRI Study of Morphometry of Menisci of Knee Joint

Introduction: Meniscal tears are commonly encountered in clinical practice and cause significant musculoskeletal morbidity. Most of the data available on Morphometry of menisci in Indian population are through cadaveric studies and very few MRI studies are available to substantiate the available data. Aim of the study: To gather data on the Morphometry of menisci in South Indian population and compare it with the existing literature. Material and Method: 100 MRI images collected retrospectively were used to study menisci. Height and width of MM and LM were measured both in sagittal and coronal planes. Results: It was observed that in both sexes the height of the Anterior horn of Medial meniscus was significantly higher than that of Lateral meniscus. Width of the Anterior horn and body showed significantly higher values in Lateral meniscus whereas width of Posterior horn of Medial meniscus showed significantly higher values in both sexes. Height of Medial meniscus in all the segments was higher in males though the difference was significant only in anterior horn and Posterior horn segments. Height of Lateral meniscus was significantly higher in males in all the segments. Conclusion: Results of this study will add to the existing literature on the Morphometry of menisci and will serve as a database for patients undergoing meniscal allografts.

Evaluation of Three-Way Cross Hybrids and Their Parents for Storage Losses in Onion (Allium cepa L.)

The aim of present investigation was the assessment of the several new onion hybrids develop through three- way cross along with their parents for having less problem of weight losses due to sprouting and rotting, and long storage quality at normal room temperature and relative humidity. The design of experimental plot was complete randomized block design (CRBD) with three replications. This study was carried out in the year of 2018-2019 at Division of Vegetable Crops, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru, India. Thirty hybrids developed by three- way cross, thirteen parental lines and one check have been included for the experiment. Three F1 hybrids used as female lines and ten commercial varieties used as male testers, were taken to develop the hybrids through line x tester design. The bulbs of all these genotypes were kept for four months in normal storage chamber at 25-30°C room temperature with 65-70% relative humidity. The overall losses of per cent bulb weigh was recorded and conclude that the three-way hybrids TWCHO-15 (26.91) were recorded for smallest amount of loss followed by TWCHO-5 (27.02), TWCHO-14 (28.83) and TWCHO-4 (29.98). These hybrids can be suggested for more profit to onion grower by keeping for long shelf-life.

Anatomical Study On Location Of Greater Palatine Foramen In Dried Adult Human Skull: Key To Successful Maxillary Nerve Block

Background: The greater palatine foramen (GPF) conducts greater palatine nerve, responsible for the innervationof posterior part of the hard palate. Anaesthetic block for greater palatine nerve is highly recommended forsurgical practices involving upper molar, maxillary sinus and nasal region. But the practical problem associatedwith anaesthesia is difficulty in locating the exact position of greater palatine foramen, leading to deliveringinsufficient anaesthetic solution. The greater palatine neurovascular structures enter the oral cavity through thegreater palatine foramen so this foramen should be approached carefully during any surgical procedures toavoid damage to these neurovascular structure.Materials and methods: 126 dried adult skull bones of unknown sex, obtained from the department of anatomy,MVJ Medical College and Research Hospital were used to locate the exact position of greater palatine foramen inrelation to bony landmarks. On both side, the distance of greater palatine foramen from midline maxillarysuture, posterior border of hardpalate, incisive fossa and lesser palatine foramen were measured with digitalvernier caliper. Location of GPF in relation to molar or premolar tooth was also noted. The data obtained wereanalyzed statistically by calculating mean and standard deviation. The percentage was calculated for the locationof GPF in relation to molar or premolar tooth.Result: In the present study of anthropometric analysis of greater palatine foramen of 126 dried skulls, it wasobserved that the most common location of greater palatine foramen was opposite to the third molar tooth. Themean distance between greater palatine foramen to mid maxillary suture was 13.71mm on right side and13.72mm on left side and posterior border of hard palate on right side was 4.62mm and 4.49mm on left side. Themean distance between greater palatine foramen and incisive fossa was 36.73mm and 36.66mm on the right andleft side respectively. The mean distance between greater palatine foramen and lesser palatine foramen was1.47mm on right side and 1.49 mm on left side.Conclusion: Results of present study may contribute greatly to the successful outcome of maxillofacial and oralsurgeries regional anaesthesia.

Cheiloscopy: Study Of Lip Prints In Establishing Identity Of An Individual

Background: The challenges faced by man in early days to provide the identity of an individual. Identity meansdetermination of distinctiveness of a person, which is very important in legal medicine and criminal investigation.Cheiloscopy is a forensic investigation that deals with the identification based on lip traces. The lip prints areindividualistic like the finger prints and do not undergo alteration during the life of an individual. The aim of thepresent study to assess the lip print pattern of different individual in different quadrant of lip and to evaluate thelip prints for their uniqueness.Materials and method:The study was conducted on 100 medical students (50male and 50 females) of 18-23years from A.J.Institute of Medical Sciences, Mangalore. Lip stick was applied on the lips and print was takenwith the help of white bond paper. While studying the lip print 4 quadrants were made on the lips as upper rightand upper left and lower right and lower left. Predominant pattern in each quadrant is obtained by using Suzuki’sclassification and result s are analyzed statistically by calculating the percentage for each pattern in eachquadrant of lip.Result: The present study showed type 1(vertical) was the overall most common pattern among both male andfemales.In males, 40% were having type1(vertical), 26.5% of type3(branched), 13.5% of type2(partial length),12% of type 5(intersected) and 8% of type4 (reticular).In females, 37.5% were having type1(vertical), 22.5% of type2(partial length), 22% of type3(branched), 11% oftype 4 (reticular),and 7% of type 5(intersected).Conclusion: Study revealed that the lip prints pattern for each individual in each quadrant is unique. Lip printpatterns are constant do not change with time.Because of its uniqueness and constant nature it can be used inthe personal identification mainly for criminal and legal investigations.

A comparative study on placebo and trenaxamic acid for the prophylaxis is of post-partum hemorrhage: a randomised double-blind control study

Background: Obstetric haemorrhage accounts for 20-25% of maternal mortality and morbidity. Anti-fibrinolytics are being widely used in field of surgery. It is also used to reduce heavy menstrual blood loss. The aim of this study was to analyse the effectiveness of TXA in reducing blood loss during normal vaginal delivery.Methods: The randomized double-blind control study was done in the Labour ward. It was conducted on 100 women undergoing Normal vaginal delivery. They were allocated to either Study or Control group by randomization. TXA was given during the Third stage of delivery in study group in addition to the routine care whereas the control group had routine care alone. Blood loss was measured in both groups by bag method.Results: The significant of reduction in blood loss calculated from placental delivery to 2hrs. 141.9 ml in study group versus 270.4 ml in control group. Among primi patients, the control group average blood loss was 325ml, the study group avg blood loss was 169ml. Among G2 patients, the control group average blood loss was 248.5ml. The study group average blood loss was 128.25ml. Among G3 patients, the control group average blood loss was 203ml, the study group average blood loss was 115ml.Conclusions: TXA significantly reduced the amount of blood loss during normal vaginal delivery. Thus, TXA can be used safely and effectively in subjects undergoing normal vaginal delivery.

Maternal outcome in placenta previa – A retrospective study

Background: Obstetric hemorrhage is one of the most dangerous and devasting group of disorders in Obstetrics of which placenta previa contributes 1/5th of the cases. The aim of this study was to analyze the maternal outcome of these cases. Materials and methods: This was a retrospective study conducted in the Department of Obstetrics and Gynecology of King George Hospital, Visakhapatnam from October 2017 to January 2018. Chart records of all women who had placenta previa were reviewed and relevant clinical findings were noted. Results: In the present study, 50 cases of placenta previa were studied regarding type of clinical presentation, clinical course, maternal outcome. Information obtained was arranged statistically. Placenta previa cases were highest in the age group 20-29 years (82%) and in multiparous group (74%). Most common risk factor was previous cesarean section (44%) followed by abortion in 24%. Major degree of placenta previa constitutes 74% i.e., majority of cases and minor degree constitutes 26% of cases. In the present study adherent placenta previa in 8% and a total of 14% required hysterectomy. Conclusions: Placenta previa poses danger to both the mother and the baby with high maternal morbidity and adverse perinatal outcome.

A Prospective Study of Cutaneous Calcifications and Clinicopathologic Correlations.

Background: Cutaneous calcifications or calcinosis cutis is an interesting lesion, wherein the skin, soft tissues and in the walls of small/medium sized veins, arteries. The aim of the present study is to evaluate the calcinosis cutis or cutaneous calcification prevalence and also its correlation with age, sex, site of the lesion, clinical features and histopathological findings. Methods: A 5 years prospective study was on 40 patients suspected to have calcinosis cutis. Surgical excision of skin lesions was also performed to do the histopathological study. Results: Women presented with lesions around the waist commonly. Males presented with lesions at different sites like over dorsum of legs and fore arm most commonly. Out of 40 cutaneous calcifications, 14 (35%) were Asymptomatic, 12 (30%) were painless papules/nodules, 8 (20%) were ulceration with or without discharge, 6 (15%) were painful papules/nodules. Conclusion: As the Cutaneous calcifications were mostly asymptomatic or painless, need to evaluate the carefully and treat if there is any underlying pathologies. Calcinosis cutis individuals should be educate and counsel regarding underlying pathologies and treatment.

Sterilisation Of Rotary Cutting Instruments – A Survey On Current Practices

Aim: The aim of this study was to obtain the information regarding present sterilization procedures followed for sterilizing Rotary cutting instruments or burs in routine dental practices using a Questionnaire and to assess the effectiveness of those procedures in preventing cross infection, and to recommend an effective sterilization protocol to be followed while using rotary cutting dental burs. Materials and Methods: A questionnaire which was pre-validated was used to collect the information regarding the methods followed to sterilize the rotary cutting instruments in 100 dental clinics in and around Hyderabad and the effectiveness of those methods were analyzed using descriptive analysis. Results: The procedures undertaken by our clinicians in their day to day practice to resterilize rotary cutting burs are poorly effective, the risk of cross-infection is very high with those methods. Conclusion: The cleaning and resterilization procedures of rotary cutting instruments that were followed regularly in clinical practice were not adequate, and more rigorous procedures are needed. If such procedures cannot be devised, these instruments should perhaps be considered single-use devices.

In vitro Anticancer Property of Yellow Pigment fromStreptomyces griseoaurantiacus JUACT 01

ABSTRACT Despite the complications in isolation of pigments, microbial pigments are increasingly gaining the attention of researchers because of their broad range therapeutic potentials, especially against cancer. In this study the cytotoxic and anti proliferative potentials of yellow pigment from Streptomyces griseoaurantiacus JUACT 01 isolated from soil are investigated. The effect of pigment treatment on the growth and proliferation of in vitro cervical cancer cells (HeLa) and liver cancer cells (Hep G2) was tested by various methods. Significant cytotoxicity was observed with IC 50 values as low as 1.5 and 1.8 µg /mL with HeLa and Hep G2 cells respectively. The pigment exhibited non toxic effects on human lymphocytes. Decrease in the number of viable cells, presence of apoptotic bodies, nuclear condensation and sheared DNA were distinctly observed in pigment treated cancer cells. The biochemical test and the infrared (IR) spectra indicated the probable carotenoid presence in the TLC purified pigment fraction. High Performance Liquid Chromatography (HPLC) analysis of the TLC purified yellow pigment showed a single large peak with a retention time of 9.90 min and m/z value corresponding to the peak was found to be 413.22 showing 100% relative abundance.

An osteological study of morphometry of hard palate and its importance.

Background: The hard palate is an essential part of human skull, the detailed knowledge of which plays an important role in the passive articulation of speech. Methods: The present study was conducted on 65 dry skulls from the department of anatomy, MVJMC & RH, Bangalore. With vernier caliper, palatine length, palatine breadth and heights were measured. Palatine index and palatine height index were calculated. Results: Mean palatine length was 48.47 ± 4.66 mm. Mean palatine breadth was 36 ± 4.41 mm and height was 8.62 ± 2.76 mm. According to the palatine index range, 66% of the hard palate belongs to leptostaphyline, 18.5% belongs to mesostaphyline and 15. 5% was brachystaphyline. As per palatine height index, 72.3% of hard palate showed chamestaphyline followed by 26.1% orthostaphyline and 1.6% hypistaphyline. Conclusions: These observations can be utilised for ethnic and racial classification of crania, anthropological studies, fabricating complete maxillary dentures for edentulous patients and performing certain surgical procedures in hard palate & soft palate.

Management and final visual outcome of various types of the lens induced glaucomas attending to Narayana Medical College Hospital.

Objectives: A clinical study of visual outcome and intraocular pressure control after cataract surgery in Lens Induced Glaucoma. Material and methods: A minimum of 50 cases of lens induced glaucoma were selected based on simple random sampling, who attended the department of Narayana Medical College Hospital and were diagnosed to be having Lens Induced Glaucoma. Appropriate medical management was given followed by surgical intervention. Patients were followed up on the 1st week, 4th week and 6th week postoperatively. Results: Finally after the analysis of the data, it was demonstrated that the longer the duration of symptoms and higher intraocular pressure (IOP) at presentation, the poorer the visual prognosis. It was also noticed that the intra-operative and postoperative complication were more in cases of LIG as compared to normal population. It was also noticed that after cataract extraction surgery few cases had post-operative IOP above borderline. These cases can be controlled by anti-glaucoma medication and regular follow-up of these cases. If necessary, trabeculectomy may be done in future to control the IOP. Conclusion: The incidence of postoperative uveitis was more in LIG when compared to the normal population and more so if associated with intra-operative complications. The visual recovery was fairly good in all those cases that have undergone treatment quite early. Also the higher the IOP pre-operatively, the prognosis for good vision following surgery is poor. A sustained rise of pressure for a long time was a bad prognostic factor for post-operative development of good vision. It is to be stressed upon, imparting health education and creating awareness regarding cataract and its implications among the rural community, ophthalmic assistants and peripheral health workers.

Cadaveric Study on the Location of Pharyngeal Orifice of an Eustachian Tube in Relation to the Anatomical Landmarks.

Introduction: Eustachian tube is a communication between the nasopharynx and middle ear extending between the lateral wall of the nasopharynx to anterior wall of tympanic cavity.It maintains the equilibrium of air pressure on either side of the tympanic membrane for proper vibration of sound. The pharyngeal orifice of Eustachian tube is an important landmark for endoscopic surgeries and also for transnasal approach to infratemporal fossa. Materials and Methods: A total of 50 (25 right &25 left)sagittal section of head and neck specimens from adult formalin fixed cadavers from the department of anatomy, MVJ Medical college and Research Hospital, Banglore, India, were used for the study.The distance of pharyngeal orifice to posterior border of hard palate,C1 vertebra,posterior choana,tip of uvula, spehenoid sinus were measured with the digital Vernier caliper.The mean and standard deviation of these parameters were calculated. Conclusion: The knowledge of the relationship of Eustachian tube with anatomical landmarks would be useful to surgeons,otolaryngologists and radiologists.

Riboflavin transporter-2 (rft-2) of Caenorhabditis elegans: Adaptive and developmental regulation.

Riboflavin transporters (rft-1 and rft-2), orthologous to human riboflavin transporter-3 (hRVFT-3), are identified and characterized in Caenorhabditis elegans. However, studies pertaining to functional contribution of rft-2 in maintaining body homeostatic riboflavin levels and its regulation are very limited. In this study, the expression pattern of rft-2 at different life stages of C. elegans was studied through real-time PCR, and found to be consistent from larval to adult stages that demonstrate its involvement in maintaining the body homeostatic riboflavin levels at whole animal level all through its life. A possible regulation of rft-2 expression at mRNA levels at whole animal was studied after adaptation to low and high concentrations of riboflavin. Abundance of rft-2 transcript was upregulated in riboflavin-deficient conditions (10 nM), while it was downregulated with riboflavin-supplemented conditions (2 mM) as compared with control (10 μM). Further, the 5 -regulatory region of the rft-2 gene was cloned, and transgenic nematodes expressing transcriptional rft-2 promoter::GFP fusion constructs were generated. The expression of rft-2 was found to be adaptively regulated in vivo when transgenic worms were maintained under different extracellular riboflavin levels, which was also mediated partly via changes in the rft-2 levels that directs towards the possible involvement of transcriptional regulatory events.

Phlorotannins from Brown Algae: inhibition of advanced glycation end products formation in high glucose induced Caenorhabditis elegans.

Advanced Glycation End products (AGE) generated in a non enzymatic protein glycation process are frequently associated with diabetes, aging and other chronic diseases. Here, we explored the protective effect of phlorotannins from brown algae Padina pavonica, Sargassum polycystum and Turbinaria ornata against AGEs formation. Phlorotannins were extracted from brown algae with methanol and its purity was analyzed by TLC and RP-HPLC-DAD. Twenty five grams of P. pavonica, S. polycystum, T. ornata yielded 27.6±0.8 µg/ml, 37.7 µg/ml and 37.1±0.74 µg/ml of phloroglucinol equivalent of phlorotannins, respectively. Antioxidant potentials were examined through DPPH assay and their IC50 values were P. pavonica (30.12±0.99 µg), S. polycystum (40.9±1.2 µg) and T. ornata (22.9±1.3 µg), which was comparatively lesser than the control ascorbic acid (46±0.2 µg). Further, anti-AGE activity was examined in vitro by BSA-glucose assay with the extracted phlorotannins of brown algae (P. pavonica, 15.16±0.26 µg/ml; S. polycystum, 35.245±2.3 µg/ml; T. ornata, 22.7±0.3 µg/ml), which revealed the required concentration to inhibit 50% of albumin glycation (IC50) were lower for extracts than controls (phloroglucinol, 222.33±4.9 µg/ml; thiamine, 263 µg/ml). Furthermore, brown algal extracts containing phlorotannins (100 µl) exhibited protective effects against AGE formation in vivo in C. elegans with induced hyperglycemia.

Study of the incidence, mechanism, various modes of presentation and factors responsible for the development of lens-induced glaucomas

Objectives: The present study undertaken to study the factors responsible for the development of lens-induced glaucomas [LIGs], factors, and various modes of presentation in LIGs

Methodology: A minimum of 50 cases of LIG were selected based on simple random sampling, who attended the Department of Narayana Medical College Hospital and were diagnosed with LIG. Based on the clinical findings, etiological diagnosis of LIG was made

Results: Out of the 50 cases, 34 cases [68%] were diagnosed as phacomorphic glaucoma, 12 cases [24%] were of phacolytic glaucoma, two each [4%] were lens-particle glaucoma and glaucoma secondary to subluxation/dislocation of lens

Conclusion: There was no influence of sex, religion, or occupation on the incidence or occurrence of LIGs. Majority of the patients had good vision in the other eye as a result of which they neglected the affected eye till they developed LIG leading to pain during the time of presentation. Hence, it is important to advice the patients regarding the early surgical treatment of cataract before they develop complications

Phytochemical screening and analysis of antioxidant properties of aqueous extract of wheatgrass

Objective: To screen the phytochemical constituents and study antioxidant properties of the aqueous extract of the wheatgrass. Methods: The current study was focused on broad parameters namely, phytochemical analysis, gas chromatography-mass spectrometry analysis and antioxidant properties in order to characterize the aqueous extract of wheatgrass as a potential free radical quencher. Results: The phytochemical screening of the aqueous extract of wheatgrass showed the presence of various secondary metabolites but the absence of sterols and quinone in general. Wheatgrass was proved to be an effective radical scavenger in all antioxidant assays. The gas chromatography-mass spectrometry analysis confirmed the presence of diverse category of bioactive compounds such as squalene, caryophyllene and amyrins in varying percentage. Conclusions: From the results obtained, we conclude that wheatgrass aqueous extract contains various effective compounds. It is a potential source of natural antioxidants. Further analysis of this herb will help in finding new effective compounds which can be of potent use in pharmacological field.

Phytochemical screening and analysis of antioxidant properties of aqueous extract of wheatgrass

OBJECTIVE@#To screen the phytochemical constituents and study antioxidant properties of the aqueous extract of the wheatgrass.@*METHODS@#The current study was focused on broad parameters namely, phytochemical analysis, gas chromatography-mass spectrometry analysis and antioxidant properties in order to characterize the aqueous extract of wheatgrass as a potential free radical quencher.@*RESULTS@#The phytochemical screening of the aqueous extract of wheatgrass showed the presence of various secondary metabolites but the absence of sterols and quinone in general. Wheatgrass was proved to be an effective radical scavenger in all antioxidant assays. The gas chromatography-mass spectrometry analysis confirmed the presence of diverse category of bioactive compounds such as squalene, caryophyllene and amyrins in varying percentage.@*CONCLUSIONS@#From the results obtained, we conclude that wheatgrass aqueous extract contains various effective compounds. It is a potential source of natural antioxidants. Further analysis of this herb will help in finding new effective compounds which can be of potent use in pharmacological field.

Genotyping of Mycobacterium leprae present on Ziehl-Neelsen-stained microscopic slides and in skin biopsy samples from leprosy patients in different geographic regions of Brazil

We analysed 16 variable number tandem repeats (VNTR) and three single-nucleotide polymorphisms (SNP) in Mycobacterium leprae present on 115 Ziehl-Neelsen (Z-N)-stained slides and in 51 skin biopsy samples derived from leprosy patients from Ceará (n = 23), Pernambuco (n = 41), Rio de Janeiro (n = 22) and Rondônia (RO) (n = 78). All skin biopsies yielded SNP-based genotypes, while 48 of the samples (94.1%) yielded complete VNTR genotypes. We evaluated two procedures for extracting M. leprae DNA from Z-N-stained slides: the first including Chelex and the other combining proteinase and sodium dodecyl sulfate. Of the 76 samples processed using the first procedure, 30.2% were positive for 16 or 15 VNTRs, whereas of the 39 samples processed using the second procedure, 28.2% yielded genotypes defined by at least 10 VNTRs. Combined VNTR and SNP analysis revealed large variability in genotypes, but a high prevalence of SNP genotype 4 in the Northeast Region of Brazil. Our observation of two samples from RO with an identical genotype and seven groups with similar genotypes, including four derived from residents of the same state or region, suggest a tendency to form groups according to the origin of the isolates. This study demonstrates the existence of geographically related M. leprae genotypes and that Z-N-stained slides are an alternative source for M. leprae genotyping.

Synthesis and characterization of silver nano particles from wrightia tinctoria.

In our present study the conventional (physical and chemical) method of nano particles synthesis was replaced with biological synthesis of silver nanoparticles by using the leaf extract of Wrightia tinctoria. New drug delivery system has been enhanced with noval techniques like nano particle synthesis and its role in drug delivery system, by exploiting the nanotechnology in particle synthesis. The aqueous extract of Wrightia tinctoria leaves was prepared and mixed with 1mM AgNO3 solution. After 48 hours the reduction of silver nitrate to silver nanoparticles (AgNPs) was confirmed by UV-visible spectrophotometer. The size of Silver nanoparticles were characterized by XRD and FTIR and the size were of 19 – 68nm. The biosynthesis of AgNPs using Wrightia tinctoria leaf extract is very simple and economic. This green chemistry approach is amenable to large scale commercial production. The use of environmentally benign and renewable plant material offers enormous benefits of eco-friendliness.