Study of p53 Expression in Carcinoma Cervix and Normal Cervical Epithelium

Introduction: Cervical carcinomas are one of the leading causes of mortality in women worldwide and various screening methods are developed for early detection, which includes clinical, pathological and molecular methods. Biomarker- p53 protein is useful in differentiating neoplastic from non-neoplastic and CIN lesions and the p53 expression is also a prognostic marker for cervical carcinoma as it correlates with other clinico-pathological prognostic parameters. Aim and objectives: To study expression of p53 in cervical carcinomas of various histological types and normal cervical epithelium and Correlation of p53 expression with the histopathological grade of cervical carcinomas. Materials and methods: 40 cases of cervical biopsy/ hysterectomy specimens of carcinoma cervix and 20 cases of hysterectomy specimens for other gynecologic causes received at department of pathology, CAIMS, Karimnagar from January 2014 to October 2015 were examined for gross and microscopic features. Immunohistochemistry was used to study the p53 expression in normal and neoplastic cervical epithelium. The p53 expression was correlated with various clinicopathological prognostic parameters. Results: Of the 40 cases of carcinoma cervix, 80% cases showed p53 positivity. The predominant histological type of carcinoma cervix positive for p53 was moderately differentiated squamous cell carcinoma (SCC). One out of three cases of Adenocarcinoma (ADC) was p53 positive. The single case of CIN III showed p53 positive cells in all the layers of squamous Radhika Mucharla, Pingle Prathyusha, Geeta Voolapalli, T. Ravinder, Vemula Sreenivas. Study of p53 Expression in Carcinoma Cervix and Normal Cervical Epithelium. IAIM, 2019; 6(7): 37-48. Page 38 epithelium. The p53 positivity showed a statistically significant association with the microscopic type of carcinoma cervix (p value=0.019). The p53 positivity increased with the age, parity, clinical stage and grade of the disease. Conclusion: The expression of p53 was high in premalignant cervical lesion in invasive cervical carcinoma compared to normal cervical epithelium. Our study indicates that p53 is a powerful prognostic marker.

Epithelioid trophoblastic tumor: a case report of a rare trophoblastic neoplasm.

Epithelioid Trophoblastic Tumor (ETT) is a rare neoplasm of the chorionic type intermediate trophoblastic cells. It is a neoplasm of reproductive age women and usually follows a gestational event. ETT can occur at both intra uterine and extra uterine sites and can be confused with other entities such as squamous cell carcinoma, placental site nodule, placental site trophoblastic tumor etc. Hence, proper diagnosis of this tumor is necessary to avoid unnecessary, excessive treatment as surgical treatment is considered sufficient for ETT. We present a case of ETT in a 36 year old female, who came with symptoms of pain abdomen, white discharge per vaginum and a cervical mass.

Cellular blue nevus – A challenging entity: Case report.

Blue nevi can present clinically as blue, gray, brown, or black solitary nodules or plaques on the skin. Histologically, they represent collections of melanocytes and melanophages in the dermis. We presented here a case of a cellular blue nevus in a 20 years old man that presented as an enlarging blue-gray nodule on the right buttock. These cases can be challenging both clinically and histologically because malignant melanoma or malignant transformation of a blue nevus could be considered.

Schwannoma of Tongue, A Rare Intraoral Neoplasm: Case Report.

Schwannomas are truly encapsulated neoplasms of the human body and are always solitary. Only 1-2% occur intraorally with tongue being the most common site. A 20yr old male presented with a painless, slow growing swelling on the left side of the tongue for the past 1 year. Fine needle aspiration cytology was done and a benign mesenchymal lesion, possibility of Schwannoma was given. Biopsy of the tumour was performed and sent for histopathological examination which confirmed the diagnosis of Schwannoma.

Primary pulmonary synovial sarcoma: a rare case report.

Primary lung sarcoma is an extremely rare tumor, accounting for less than 0.5% of all lung tumors. Histological subtypes are differentiated on the basis of immunohistochemical markers, such as vimentin, desmin, actin, CD99, and epithelial membrane antigen. A 50-year-old male presented with progressively increasing shortness of breath with cough for 2 months. On Contrast Enhanced Computed Tomography (CECT) of thorax a large heterogeneous mass with multiple areas of necrosis, occupying almost whole of left hemithorax was seen. CT-guided Fine Needle Aspiration Cytology (FNAC) revealed spindle cell neoplasm. Histopathological examination revealed a spindle cell sarcoma. On immunohistochemistry the tumor cells expressed both epithelial membrane antigen and vimentin. Hence, final impression from immunohistochemistry was primary monophasic synovial sarcoma of lung.