RESUMO
The object of study is the complex plant remedy that has been developed on the basis of Tibetan medicine recipes. In experimental dyslipidemias induced by atherogenic diet the course preventive introduction of the plant remedy decreases the content of blood proinflammatory cytokines and acute phase proteins Key words: complex phytoremedy, dyslipidemia, proinflammatory cytokines, acute phase proteins. Introduction: Cardiovascular and cerebrovascular diseases associated with atherosclerosis were the leading causes of morbidity and mortality, and at the present stage they have reached epidemic proportions [14]. Dyslipidemia is known to be the undoubted leader among other risky factors of atherosclerosis which pathophysiological importance is bouns with influence of high concentration of lipoproteins (especially their modified variants) on different cellular elements of vessel wall, primarily on endothelium [11]. As a reaction to such effect endothelium can cardinally change its metabolism demonstrating proinflammatory activation expression of proinflammatory cytokines. In this respect dyslipidemia is the factor accelerating the process of tissue damage together with other phlogogens such as mechanic and chemical damage of parts under the influence of medication, nicotine, bacterial toxins, protease and other [7]. In the traditional medical systems of Eastern Asia countries, including Tibet, a disease is considered as a disturbance of the whole body functioning, therefore, therapy is aimed at the regulation of the structural and functional organization of the body as an entity. Such position substantiates the use of multicomponent plant remedies in pharmacotherapy of various diseases [8]. Complex plant remedies surely have some advantages over one-component preparations. In particular, they have versatile effect on the body due to the complex and balanced chemical composition and rational combination of biologically active substances. On the one hand they directly influence the location of injury, on the other hand they provide pharmacological correction of various functional systems, as well as they increase the resistance of the whole body. Besides, the plants when used in the complex manifest synergism, which strengthens useful properties of ingredients included in their composition. In contrast to xenobiotics biologically active substances of various chemical groups contained in the plants provide not only their polyvalent effect and complex influence but also a maximal biological accessibility. These properties provide a more gentle effect, high efficiency, good interaction and absence of adverse effects when plant preparations are used for a long time [13]. The aim of the research: Study of complex phytoremedy influence on the content of proinflammatory cytokines and acute phase proteins in experimental dyslipidemia. Materials and methods: The subject of the study was the complex plant remedy, composed on the base of formulae from the “Chgud-zhi” [5]. The given remedy includes: Calendula officinalis L. (flowers), Crataegus sanguinea Pall (fruits), Scutellaria baicalensis Georgi (roots), Myristica fragrans Houtt. (seeds), Malus baccata (L.) Borkh. (fruits), Rhamnus davurica Pall. (bark), Valeriana officinalis L. (roots), Glycyrrhiza uralensis Fisch. (rhizomes), Rosa sp. (flowers and fruits), Cetraria islandica (L.) Ach. (blastema) and others. The experiments were carried out in 24 Wistar rats with the initial weight of 170-190 g. The animals were kept in standart conditions of vivarium at the same diet and care, light and temperature regiment. Experimental studies were carried out according to the Regulations of European convention concerning the protection of vertebrates used for experimental and other scientific purposes (Strasbourg, 1986). All the animals were divided into 3 groups: 1- intact group of rats; 2- control group of rats on atherogenic diet for 12 weeks; 3- experimental group of rats that receive prophylactic course phytoremedy. The tested remedy in the form of decoction was introduced intragastrically in the dose of 1 ml/100 g of the weight once a day during the whole period of the experiment. The control group of animals received the same volume of distilled water [6]. Levels of proinflammatory cytokines - interleukin-1beta (IL-1β), interleukin-(IL-6) and tumor necrosis factor-alpha (TNF-α) in the serum were determined by ELISA using kits “Vector-Best”. C-reactive protein (CRP) in serum was defined by latex agglutination, fibrinogen concentration in plasma of method according Rutberg [8].The data obtained were statistically processed by generally used methods; the findings were estimated with the use of Student’s criterion [12].