A Sensitive Ultra-Fast Bioanalytical Method for the Quantification of Rabeprazole in Human Plasma.

A highly sensitive ultra-fast LC-MS/MS based bioanalytical method for the measurement of Rabeprazole in human plasma was developed and validated using 13C-D3-Rabeprazole as internal standard. Rabeprazole is a sulfabenzimidazole class of compounds, setting chromatography for these classes of compounds is always a challenge. Rabeprazole was extracted from human plasma samples by liquid-liquid extraction (LLE) and separated on a short reverse phase Ascentis® Express C18, 50 mm × 4.6 mm, 2.7 μm column by isocratic elution with 40% 10 mM ammonium acetate solution and 60% acetonitrile at a flow rate of 0.700 mL/min. Rabeprazole and its labeled internal standard were detected by multiple reaction monitoring (MRM) mode using electro spray ionization (ESI). All the validation parameters as per current guidelines like specificity, selectivity, accuracy, precision, recovery, matrix factor, haemolysis effect and stability are assessed in human plasma. Rabeprazole was found to be linear over a range of 0.1 ng/mL to 150 ng/mL in human plasma. LLOQ of0.1 ng/mL is sensitive enough for application to different clinical studies. The intra- and inter-day precision was less than 10% and accuracy was within -3.33 to 10.00%. Recovery (70%) was consistent across the linear dynamic range of the method. The validated method is a simple, accurate, precise and robust to measure Rabeprazole in human plasma and could be used for application to any format of clinical studies.

Immobilization technique of natural dyes, as a novel method to preserve industrially important E. coli and Bacillus species.

Immobilization is a method used for the preservation of enzymes as this method provides enhanced resistance to the changes in certain environmental conditions like pH/temperature. The advantage of immobilization (IMB) of live bacterial cells is especially high due to their role in environmental monitoring due to their low cost, easy handling and high sensitivity to the environment. Through our project we tried to show that this method of IMB is effective in the preservation of microorganisms. We used natural dyes during the course of our project which were extracted from grinding of fruits and vegetables such as pomegranate, beetroot and carrot. We used Sodium Alginate beads so that there were a good number of beads formed that helped for the proper entrapment of microorganisms E. coli and bacillus for future use. Natural dyes were chosen over synthetic dyes due to their environmental friendliness, cost effectiveness and less complexity. To confirm if the method had been effective, we prepared a growth curve to check the growth of the microorganism and found out that this method could be used to store the microbes for a month’s duration.

The role of microbial pullulan, a biopolymer in pharmaceutical approaches: A review.

The article presents an overview of the latest advances in investigations and application of the pullulan from microbes (Auriobasidium sps.) and its derivatives in the biomedical and pharmaceutical field and how this polysaccharide can be of use as a promising biomaterial in the coming future. So many papers were published during the last four decades indicated that pullulan the unique biomaterial which has so many applications in food, cosmetics, biomedical and pharmaceutical industries . The unique linkage α (1→4) and α (1→6) pattern of pullulan endows the polymer with distinctive physical traits, including adhesive properties and the capacity to form fibers. Due to its non-toxic, non-immunogenic, non-mutagenic and non-carcinogenic nature recently there is an attempt to explore this polysaccharide for various medical applications including targeted drug and gene delivery, tissue engineering, wound healing and also even in diagnostic applications like perfusion, receptor, and lymph node target specific imaging and vascular compartment imaging using it as quantum dots.