Lack of Association of Common Polymorphism of LRP1 Gene with Myocardial Infarction in a Chinese Han Population / 华中科技大学学报（医学）（英德文版）

This study examined the association of a common polymorphic allele (25G) of the low-density lipoprotein receptor-related proteinl (LRP1) gene with myocardial infarction (MI).The genotypes of LRP1 25CG (rs35282763) were determined in 347 MI patients and 347 age-and sex-frequency-matched controls from an unrelated Chinese Han population.Factor Ⅷ (FⅧ) levels were measured in the MI patients and controls by chromogenic assay and enzyme-linked immunosorbent assay (ELISA).The results showed that LRP1 25CG (rs35282763) genotype distribution did not differ significantly between patients (n=206 for 25CC,n=122 for 25CG) and controls (n=191 for 25CC,n=126 for 25CG;P＞0.05).The 25G allele was not associated with a reduced risk of MI (P ＞0.05).Further stratifications for age,sex,and other cardiovascular risk factors did not affect the negative findings.It was concluded that the presence of the G allele at the 25CG (rs35282763) polymorphism of the LRP1is not associated with a reduced risk of MI,and genotyping for LRP1 25CG (rs35282763) polymorphism is not useful in assessing the individual risk of MI.

Binding of EGF1 Domain Peptide in Coagulation Factor Ⅶ with Tissue Factor and Its Implications for the Triggering of Coagulation / 华中科技大学学报（医学）（英德文版）

The binding function of EGF1 domain peptide with tissue factor(TF)and its ability of triggering coagulation were explored.The TF expression model in vitro was established by lipopolysaccharide induction.The affinity of EGFP-EGF1 and TF expressing cells was analyzed by fluorescence microscopy and flow cytometry(FCM).The affinity of EGFP-EGF1 and rat soluble TF was quantitated by surface plasmon resonance(SPR).The ability of EGFP-EGF1 in triggering coagulation was tested by prothrombin time assay.The FCM results showed recombinant factor Ⅶ(rFⅦ)could definitely depress the integration of EGFP-EGF1 with recombinant TF(rTF)(68.65%±3.86% vs 57.98%±4.71%,P<0.01).The SPR results indicated the association constant ka of EGFP-EGF1 proteins was higher than rFⅦ(8.29±1.39 vs 3.75±0.32,P<0.01).However,the EGFP-EGF1 protein lost the activity of triggering coagulation as compared with blood plasma of normal SD rats(56.8±3.2 s vs 17.8±3.4 s,P<0.01).It was concluded that the rat EGF1 peptide could specifically bind to TF without the ability of triggering coagulation.EGF1 peptide may be a good target head for delivering drugs to TF in anticoagulation therapy.