Efectos de las diluciones homeopáticas altas sobre los modelos in vitro: revisión de la literatura / Effects of high homeopathic dilutions on in vitro models: a review of the literature

Los efectos de las diluciones homeopáticas altas (HD, por sus siglas en inglés) son controvertidos debido a que superan el número de Avogadro. Objetivo: Realizar una revisión de la literatura sobre los efectos de las HD sobre los modelos in vitro. Métodos: Se realizó una búsqueda sistemática en la base de datos PubMed de estudios que evaluaran las HD simples sobre modelos in vitro, publicados de 2007 en adelante. Resultados: 28 publicaciones cumplieron con los criterios de inclusión/exclusión. 26 estudios mostraron efectos evidentes de las HD simples sobre modelos in vitro. La mayor parte de tales estudios fueron realizados en países en donde la homeopatía ha alcanzado un alto nivel de institucionalización. Conclusiones: Los modelos in vitro evidencian de forma clara la actividad biológica de las HD superiores al número de Avogadro y dan cuenta de los efectos encontrados en la práctica clínica. La mayor parte de los estudios fueron realizados en países en donde la homeopatía es reconocida oficialmente, lo cual facilita el acceso a recursos para el desarrollo de la investigación.

the effects of homeopathic high dilutions (HDs) are controversial because they exceed Avogadro's number. Aim: to perform a literature review on the effects of HDs on in vitro models. Methods: a systematic search was performed in database PubMed for studies assessing simple HDs on in vitro models published from 2007 onward. Results: 28 publications met the inclusion/exclusion criteria; 26 studies demonstrated patent effects of simple HDs on in vitro models; most such studies were conducted in countries where homeopathy attained a high level of institutionalization. Conclusions: in vitro models patently evidence biological activity of HDs above Avogadro's number and account for effects found in clinical practice. Most studies were conducted in countries where homeopathy is officially recognized, which facilitates access to resources for the development of research.

Investigación clínica en homeopatía: revisiones sistemáticas y estudios clínicos aleatorizados / Clinical research in homeopathy: systematic reviews and randomized clinical trials

Se considera que las revisiones sistemáticas y estudios clínicos aleatorizados (RCT) presentan el nivel de evidencia más alto. Objetivo: Realizar una revisión descriptiva de las revisiones sistemáticas y estudios clínicos controlados aleatorizados (RCT, por sus siglas en inglés) sobre la efectividad y eficacia de la homeopatía. Métodos: Los datos del reporte publicado por la Liga Medicorum Homeopathica Internationalis (LMHI) en 2014 fueron actualizados mediante una búsqueda en la base de datos PubMed. Resultados: Se localizaron 7 revisiones sistemáticas con meta-análisis, 6 de las cuales concluyeron que los efectos de la homeopatía no son compatibles con el efecto placebo; sólo una revisión sistemática llegó a la conclusión opuesta, aunque fue severamente criticado debido a sus errores metodológicos. Un total de 19 RCT fueron publicados a lo largo del periodo analizado. El 84.2% tuvo por lo menos un resultado positivo. Conclusiones: Con base en las evidencias disponibles del más alto nivel, no es posible afirmar que los efectos de la homeopatía son exclusivamente un efecto placebo. Por el contrario, se han detectado efectos específicos en varios estudios.

Systematic reviews and randomized clinical trials (RCT) are considered to have the highest level of evidence. Aim: To perform a descriptive review of systematic reviews and RCT on the effectiveness and efficacy of homeopathy. Methods: Data from the report published by Liga Medicorum Homeopathica Internationalis (LMHI) in 2014 were updated by means on a search conducted in database PubMed. Results: 7 systematic reviews with meta-analysis were located, 6 of them concluded that the effects of homeopathy are not compatible with placebo effect; only 1 systematic review arrived to the opposite conclusion, but was severely criticized due to methodological flaws. A total of 19 RCT were published along the analyzed period; 84.2% had at least one positive outcome. Conclusions: Based on the available evidences of the highest level it is not possible to assert that the effects of homeopathic are exclusively placebo effect. On the opposite, specific effects were detected in several studies.

Spread of homeopathy in the early nineteenth century: the comparative approach and the cases of Sweden and Brazil / A difusão da homeopatia no início do século XIX: abordagem comparativa e os casos da Suécia e do Brasil

Abstract The comparative approach has been advocated to overcome some flaws inherent to case studies. Here, the spread of homeopathy in the early nineteenth century is addressed through a comparison of the cases of Sweden and Brazil, where homeopathy met diametrically opposed fates. The parameters used for the comparison are the standard for studies on the early spread of homeopathy, such as the concept of the "introducer," and reception by the medical and academic community, the government, and society at large. The results suggest that analysis of contexts, determinants, and the interactions of practitioners and institutions representing different health care approaches, whether dominant or alternative, seems to provide a more accurate picture of different moments in the global history of medicine.

Resumo A abordagem comparativa foi adotada com o intuito de suplantar algumas falhas inerentes aos estudos de caso. Nela, a difusão da homeopatia no início do século XIX é tratada por meio de uma comparação entre casos da Suécia e do Brasil, onde a homeopatia encontrou destinos diametralmente opostos. Os parâmetros usados para a comparação são padrão para estudos sobre o início da expansão da homeopatia, como o conceito de "introdutor", e a aceitação por parte de governo, comunidades médica e acadêmica, e sociedade como um todo. Os resultados sugerem que a análise de contextos, de determinantes, e das interações de médicos e instituições representando diferentes perspectivas de tratamento médico, fossem elas dominantes ou alternativas, parecem oferecer uma análise mais precisa de diferentes momentos da história global da medicina.

High-diluted thymulin on Ehrlich tumor growth in mice and the importance of tumor microenvironment

Introduction: The aim of the present study was to describe different biological aspects of Ehrlich tumor in mice, such as body weight evolution, tumor growth rate, histological organization and systemic immune response after treatment with high-diluted thymulin (10-9 M, named 5CH). Methods: Tumor assessment was focused on macro- and microscopic aspects; parameters included occurrence of necrosis, embolism and tumor development, in addition to quantitative analysis of apoptosis (caspase-3), cell proliferation (Ki-67) and angiogenesis (vascular endothelial growth factor - VEGF) by means of specific immunohistochemistry markers. Spleen cell populations were evaluated by flow cytometry analysis. Results: Mice treated with thymulin 5CH exhibited changes in the tumor microenvironment, such as reduced micro-embolism incidence and cytokeratin expression, with increased caspase-3 expression in the tumor cells. These findings indicate some apoptotic activity by the tumor cells induced by the treatment, even though no reduction of the macroscopic tumor mass occurred. No changes in the systemic immune response were detected, as the balance among spleen cell populations remained unchanged. Conclusions: The results indicate that treatment of mice bearing Ehrlich tumor with thymulin 5CH induces some specific changes in the tumor environment. However, it did not influence systemic immunity parameters. Adjuvant use of thymulin 5CH in oncological clinical practice is still a matter of discussion. (AU)

Effects of homeopathic high dilutions on in vitro models: literature review

BACKGROUND: the effects of homeopathic high dilutions (HDs) are controversial because they exceed Avogadro’s number. AIM: to perform a literature review on the effects of HDs on in vitro models. METHODS: a systematic search was performed in database PubMed for studies assessing simple HDs on in vitro models published from 2007 onward. RESULTS: 28 publications met the inclusion/exclusion criteria; 26 studies demonstrated patent effects of simple HDs on in vitro models; most such studies were conducted in countries where homeopathy attained a high level of institutionalization. CONCLUSIONS: in vitro models patently evidence biological activity of HDs aboveAvogadro’s number and account for effects found in clinical practice. Most studies were conducted in countries where homeopathy is officially recognized, which facilitates access to resources for the development of research.

Novas evidências documentais para a história da homeopatia na América Latina: um estudo de caso sobre os vínculos entre Rio de Janeiro e Buenos Aires / New documental evidence on the history of homeopathy in Latin America: a case study of links between Rio de Janeiro and Buenos Aires

Resumo A homeopatia começou a propagar-se logo após sua formulação por Samuel Hahnemann, nos primeiros anos do século XIX, chegando ao Cone Sul na década de 1830. Esse processo é tradicionalmente vinculado à figura de um “introdutor”, por vezes alcançando estatuto mítico. No entanto, pouco se sabe acerca da chegada da homeopatia à Argentina nesse período. Com base em trabalho de arquivo, identificamos uma clara circulação de homeopatas médicos e leigos no eixo Rio de Janeiro-Buenos Aires. Dada a conhecida atividade proselitista desenvolvida nos círculos ligados aos homeopatas leigos B. Mure e J.V. Martins no Rio de Janeiro, a documentação disponível aponta para a possível extensão desse movimento também na Argentina, o que não tinha sido evidenciado até o presente.

Abstract Homeopathy began to spread soon after it was formulated by Samuel Hahnemann in the early 1800s, reaching the Southern Cone in the 1830s. In processes of this kind, one figure is often cited as being responsible for introducing it, often attaining quasi-mythical status. Little is known, however, about how homeopathy reached Argentina at that time. Through archival research, we discovered that medical and lay homeopaths circulated between Rio de Janeiro and Buenos Aires. Given the well-known proselytizing of the circles gravitating around lay homeopaths B. Mure and J.V. Martins in Rio de Janeiro, the documents indicate that this movement actually went as far as Argentina, which had not been confirmed until now.

Raízes do vitalismo francês: Bordeu e Barthez, entre Paris e Montpellier / The roots of French vitalism: Bordeu and Barthez, between Paris and Montpellier

Analisa as propostas classificadas como vitalistas, formuladas na França no século XVIII. Contextualiza a tradição da escola médica de Montpellier, abordando as concepções fisiológico-médicas de Théophile de Bordeu. Nesse ambiente Paul-Joseph Barthez realizou sua formação original. Sua concepção sobre a autonomia da vida também foi influenciada pela interação com círculos dos enciclopedistas de Paris. No entanto, na formulação desse conceito identificam-se igualmente ruptura e permanência com relação a ambas as linhas de pensamento - a respeito do conceito de ser humano na classificação das ciências prescrita pela Encyclopédie e na tradição típica de Montpellier.

This article analyzes several French eighteenth century physiological theories that later on were classified as vitalist. The overall background is set by the tradition of Montpellier medical school, in particular by the physiological and medical ideas of Théophile de Bordeu. Paul-Joseph Barthez was initially trained in this setting, however, his conception of the autonomy of life was also heavily influenced by the circle of Paris encyclopedists. For this reason, Barthez's elaboration shows elements of continuity and discontinuity regarding both the notion of human being as represented in the classification of sciences of the Encyclopédie, and the typical Montpellier.

In vitro growth of uropathogenic Escherichia coliisolated from a snow leopard treated withhomeopathic and isopathic remedies: a pilot study / Estudo do crescimento (in vitro) de Escherichia coli uropatogênica isolada a partir de um leopardo das neves e tratada com medicamentos homeopáticos e isopáticos: estudo piloto

This paper reports the results of incubation of a strain of uropathogenic Escherichia coli (UPEC) isolated from a snow leopard - which had died of septicemia secondary to necro-hemorrhagic cystitis - with homeopathic and isopathic remedies. Methods: UPEC was isolated from heart blood and previously typified for virulence factors; it was incubated with homeopathic remedies Cantharis vesicatoria (urinary tract infection affinity), Mercurius solubilis (from symptoms analysis) and nosode prepared from the actual strain, all in dilution 12cH. Results: 2 patterns of bacterial growth were observed, associated to the quality of nutrients in the culture medium; inrich-nutrient medium, nosode of E. coli 12cH had a significant inhibitory effect; in poor-nutrient medium, Merc 12cH exerted significant inhibitory effect. Conclusion: results suggest that the previous conditions of prokaryote systems may influence the in vitro response to homeopathic and isopathic remedies.

Este artigo relata os resultados da incubação de uma linhagem de Escherichia coli uropatogênica (UPEC) isolada a partir de um leopardo das neves, que morreu de septicemia secundária a cistite necrótica-hemorrágica. A UPEC foi tratada com preparados homeopáticos e isopáticos. Métodos: UPEC foi isolada de sangue cardíaco e previamente tipificada para fatores de virulência; foi incubada com o medicamento homeopático Cantharis vesicatoria (afinidade com infecção do trato urinário), Mercurius solubilis (a partir da análise de sintomas) e nosódio preparado a partir da mesma linhagem de bactérias, todas em 12 cH. Resultados: 2 padrões de crescimento bacteriano foram observados, associados à qualidade dos nutrientes do meio de cultura; em meios ricos em nutrientes, nosódio de E. coli 12 cH teve um significativo efeito inibitório; em meio pobre de nutrientes, Merc 12 cH exerceu efeito inibitório significativo. Conclusão: os resultados sugerem que as condições prévias do sistema procarioto estudado podem influenciar as respostas proliferativas in vitro para preparados homeopáticos e isopáticos.

The emergence of modern therapeutic similarity / O surgimento da semelhança terapêutica moderna

One of the roots of modern therapeutic similarity might be retraced to the work of Samuel Hahnemann at the end of the 18th century. His particular formulation arose from an original synthesis of traditional therapeutic similarity, dating from classic Antiquity and maintained as an undercurrent in medical thinking, and contemporary theories, particularly counter-irritation (antagonistic fever) theory. Against historical readings asserting that modern therapeutic similarity is either a mere a continuation of the ancient, or a historical orphan, it is possible to verify that Hahnemann's work belonged within the specific framework of 18th medical science. The formulation of modern therapeutic similarity is best described as a process combining both continuity and epistemological break.

Uma das raízes da semelhança terapêutica moderna pode ser localizada na obra de Samuel Hahnemann, no final do século XVIII. Sua formulação particular surgiu da síntese original da semelhança terapêutica tradicional, procedente da Antigüidade clássica e conservada subterraneamente no pensamento médico, com teorias contemporâneas, especialmente a teoria da contra-irritação (febre antagonista). Por oposição a leituras históricas que afirmam que semelhança terapêutica moderna é uma mera continuação da antiga ou, alternativamente, um ófão histórico, pode-se constatar que a obra de Hahnemann corresponde ao marco específico da ciência médica do século XVIII. A formulação da semelhança terapêutica parece ser melhor compreendida como um processo que combina continuidade e ruptura epistemológicas.