RESUMO
<p><b>OBJECTIVE</b>To isolate the wild-type virulent phage of Helicobacter pylori (Hp) and simulate the treatments in vitro to investigate the methods for oral Hp-assisted penetration of the phage through the gastric barrier and offspring phage release for infection and treatment of gastrointestinal Hp.</p><p><b>METHODS</b>The Hp strain was cultured with the candle cylinder method and the virulent phage was isolated by single plate or double plate experiment. A simulated gastric juice was applied and the bactericidal effect of the phage was tested with double flats experiment.</p><p><b>RESULTS</b>After a 1.5-h treatment in simulated gastric juice, the orally derived Hp-borne phage was still capable of forming plaques while the control phage was not.</p><p><b>CONCLUSION</b>The oral Hp can help the phage resist the gastric juice and then infect the gastrointestinal Hp.</p>
Assuntos
Humanos , Bacteriófagos , Fisiologia , Trato Gastrointestinal , Microbiologia , Infecções por Helicobacter , Terapêutica , Helicobacter pylori , Virologia , VirulênciaRESUMO
<p><b>OBJECTIVE</b>To discuss the possible mechanism of drug resistance transmission between Staphylococcus and Escherichia coli.</p><p><b>METHODS</b>The chloramphenicol resistance plasmid of Staphylococcus aureus was extracted to transform the sensitive Escherichia coli, and the drug-resistant Escherichia coli were screened by drug sensitivity test.</p><p><b>RESULTS</b>The drug-resistant Escherichia coli were successfully obtained.</p><p><b>CONCLUSION</b>Staphylococcus may have a natural shuttle plasmid of drug resistance, which can transform Escherichia coli under specific conditions.</p>