Knowledge, attitudes and practices regarding tuberculosis prevention and control:analysis of influencing factors of prevention and control behavior among college students in Wuhan, China / 中国学校卫生

Objective@#To determine the knowledge, attitudes and practices (KAP) regarding tuberculosis prevention and control among college students in Wuhan, China, while analyzing the factors affecting tuberculosis prevention behavior.@*Methods@#Referring to the National KAP Questionnaire for Tuberculosis Prevention and Control among College Students, which was designed by China Center for Disease Control and Prevention, 22 colleges and universities in Wuhan were selected using a judgment sampling method, and a questionnaire survey was carried out through the online questionnaire platform. The formation of tuberculosis prevention and control behavior among this population was analyzed by single factor and multi factor Logistic regression.@*Results@#The total awareness rate of KAP regarding tuberculosis prevention and control among college students in Wuhan was 79.69%; the awareness rate of core knowledge was 79.28%, and the passing rate was 92.46%. The correct attitude holding rate was 89.69%, while the passing rate was 90.56%. In respect to correct behavior, the formation rate was 72.11%, with a passing rate of 96.62%. The multivariate Logistic regression analysis showed that the following factors were more likely to promote tuberculosis prevention and control behaviors that met the target: female college students ( OR =1.86); college students whose father had a primary school education level, junior high school and equivalent, high school and equivalent, and junior high school students ( OR =2.94, 3.05, 3.17, 3.24 ); no history of tuberculosis ( OR =3.32); the passing knowledge of core knowledge ( OR =9.91), and the passing attitude holding score ( OR =7.35)( P <0.05).@*Conclusion@#The overall awareness rate of KAP regarding tuberculosis prevention and control among college students in Wuhan is acceptable,the mainly influenced factors are gender, history of tuberculosis, awareness rate of core knowledge about prevention and control, attitudes and cognition, and parents educational background.

Effects of high-intensity training on endurance in basketball players / Efeitos do treinamento de alta intensidade na resistência dos jogadores de basquetebol / Efectos del entrenamiento de alta intensidad en la resistencia de los jugadores de baloncesto

ABSTRACT Introduction: The aerobic endurance training of sports athletes has become the focus of research in institutions and related units. One of the recent proposals is high-intensity interval training to develop athletic aerobic endurance. Objective: Study the effect of high-intensity interval training on aerobic endurance in basketball players. Methods: Fifteen athletes from a university were randomly selected and divided into a high-intensity interval training group, a moderate-intensity continuous training group, and a low-intensity continuous training group, with the control of external influence factors. The indicators analyzed were the average aerobic endurance index of maximal oxygen consumption, average anaerobic capacity, and average maximal power output. The data were obtained before and after the experiment. They were compared and discussed using statistical analysis. Results: The average maximal oxygen consumption in the three training groups increased significantly, and the effect was most evident under the high-intensity intermittent training conditions. This training method could increase the maximal exercise time of the normal groups by up to two times. Conclusion: Intermittent high-intensity training can effectively improve the aerobic endurance of basketball players. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: O treinamento da resistência aeróbica dos atletas esportivos tornou-se o foco na pesquisa de instituições e unidades relacionadas. No intuito de desenvolver a resistência aeróbica atlética, uma das propostas recentes é o treinamento intervalado de alta intensidade. Objetivo: Estudar o efeito do treinamento intervalado de alta intensidade sobre a resistência aeróbica nos jogadores de basquetebol. Métodos: Foram selecionados aleatoriamente 15 atletas de uma universidade, que foram divididos em um grupo de treinamento de intervalo de alta intensidade, um grupo de treinamento contínuo de intensidade moderada e um grupo de treinamento contínuo de baixa intensidade, com o controle dos fatores de influência externa. Entre os indicadores analisados estão o índice de resistência aeróbica média de consumo máximo de oxigênio, a capacidade anaeróbica média e a potência máxima média. Os dados foram obtidos antes e após o experimento. Foram comparados e discutidos através de análise estatística. Resultados: O consumo máximo médio de oxigênio nos três grupos de treinamento aumentou significativamente, e o efeito foi mais evidente sob as condições de treinamento intermitente de alta intensidade. Este método de treinamento apresentou o potencial de aumentar o tempo máximo de exercício dos grupos normais em até duas vezes. Conclusão: O treinamento intermitente de alta intensidade pode efetivamente melhorar a resistência aeróbica dos jogadores de basquete. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: El entrenamiento de la resistencia aeróbica de los atletas deportivos se ha convertido en el centro de la investigación en instituciones y unidades relacionadas. Para desarrollar la resistencia aeróbica atlética, una de las propuestas recientes es el entrenamiento de intervalos de alta intensidad. Objetivo: Estudiar el efecto del entrenamiento interválico de alta intensidad sobre la resistencia aeróbica en jugadores de baloncesto. Métodos: Se seleccionaron aleatoriamente quince atletas de una universidad y se dividieron en un grupo de entrenamiento por intervalos de alta intensidad, un grupo de entrenamiento continuo de intensidad moderada y un grupo de entrenamiento continuo de baja intensidad, con el control de los factores de influencia externos. Entre los indicadores analizados estaban el índice medio de resistencia aeróbica de consumo máximo de oxígeno, la capacidad anaeróbica media y la potencia máxima media. Los datos se obtuvieron antes y después del experimento. Se compararon y discutieron mediante un análisis estadístico. Resultados: El consumo medio máximo de oxígeno en los tres grupos de entrenamiento aumentó significativamente, y el efecto fue más evidente en las condiciones de entrenamiento intermitente de alta intensidad. Este método de entrenamiento presentaba el potencial de aumentar el tiempo máximo de ejercicio de los grupos normales hasta dos veces. Conclusión: El entrenamiento intermitente de alta intensidad puede mejorar eficazmente la resistencia aeróbica de los jugadores de baloncesto. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Explosive strength of the lower limbs in rope skipping exercise applied to children and adolescents / Força explosiva dos membros inferiores no exercício de pular corda aplicado às crianças e adolescentes / Fuerza explosiva de los miembros inferiores en el ejercicio de salto de cuerda aplicado a niños y adolescentes

ABSTRACT Introduction: The physical health of children and adolescents is directly related to school physical training quality. The improvement of adult sports effectiveness results from a concise protocol with standardized exercises. Rope skipping exercise lacks kinematic studies applied to children and adolescents. Objective: Provide a theoretical reference for jump rope exercise training for children and adolescents. Methods: Eight children aged six to eight years old were recruited to jump at a frequency of 80 and 120 jumps per minute controlled by a metronome. The technique was divided into three stages: impulse, suspension, and landing. Control in the knee joint exoskeleton balance was investigated by computerized baropodometry with differentiation of rotational angular velocity by rotational angular acceleration via Butterworth filter. After noise cleaning and multiplication, an adjustable amplification factor returned to the inlet with inertial compensation torque. Results: Initially, the impulsing force is about three times the individual's gravity. This value and the center of mass acceleration decrease in time. The initial force is proportional to the velocity of its decrease during the impulse, and the opposite occurs with the landing force. Higher intensity is observed when raising the frequency. Conclusion: The fluctuation in the force curve in the landing phase after 20 jumps indicates low muscle strength in lower limbs to maintain activity. A speed of 80 jumps per minute controlled by music in a playful environment is recommended for children. Evidence Level II; Therapeutic Studies - Investigating the results.

RESUMO Introdução: A saúde física das crianças e adolescentes está diretamente relacionada à qualidade do treinamento físico escolar. O aprimoramento da eficácia esportiva adulta é resultado de um protocolo conciso com exercícios padronizados. O exercício de pular corda carece de estudos cinemáticos aplicados em crianças e adolescentes. Objetivo: Fornecer uma referência teórica para o treinamento físico de pular corda para crianças e adolescentes. Métodos: Foram recrutadas 8 crianças com idade entre 6 a 8 anos para saltos em frequência de 80 e 120 pulos por minuto controlados por um metrônomo. A técnica foi dividida em três estágios: impulso, suspensão e pouso. O controle no balanço do exoesqueleto articular do joelho foi pesquisado por baropodometria computadorizada com diferenciação de velocidade angular rotacional pela aceleração angular rotacional via filtro de Butterworth, após limpeza de ruídos e multiplicação por fator de amplificação ajustável, devolvidos à entrada de admissão com torque de compensação inercial. Resultados: Inicialmente, a força de impulso é cerca de três vezes a gravidade de cada indivíduo. Esse valor e a aceleração do centro de massa diminuem com o tempo. A força inicial é proporcional a velocidade da sua diminuição durante o impulso, ocorrendo o inverso com a força de pouso. Observa-se uma maior intensidade ao elevar a frequência. Conclusão: A flutuação na curva de força na fase de pouso após 20 pulos indica baixa força muscular em membros inferiores para manter a atividade. Recomenda-se a velocidade de 80 pulos por minuto controlada por música em ambiente lúdico para crianças. Nível de evidência II; Estudos Terapêuticos - Investigação de Resultados.

RESUMEN Introducción: La salud física de los niños y adolescentes está directamente relacionada con la calidad del entrenamiento físico escolar. La mejora de la eficacia deportiva del adulto es el resultado de un protocolo conciso con ejercicios estandarizados. El ejercicio de salto de cuerda carece de estudios cinemáticos aplicados a niños y adolescentes. Objetivo: Proporcionar una referencia teórica para el entrenamiento de ejercicios de salto de cuerda para niños y adolescentes. Métodos: Ocho niños de entre 6 y 8 años fueron reclutados para saltar a una frecuencia de 80 y 120 saltos por minuto controlados por un metrónomo. La técnica se dividió en tres etapas: impulso, suspensión y aterrizaje. El control sobre el equilibrio del exoesqueleto de la articulación de la rodilla se investigó mediante baropodometría computarizada con diferenciación de la velocidad angular rotacional por la aceleración angular rotacional a través del filtro Butterworth, después de la limpieza del ruido y la multiplicación por el factor de amplificación ajustable, devuelto a la entrada con par de compensación inercial. Resultados: Inicialmente, la fuerza de empuje es aproximadamente tres veces la gravedad de cada individuo. Este valor y la aceleración del centro de masa disminuyen con el tiempo. La fuerza inicial es proporcional a la velocidad de su disminución durante el impulso, y lo contrario ocurre con la fuerza de aterrizaje. Se observa una mayor intensidad al aumentar la frecuencia. Conclusión: La fluctuación de la curva de fuerza en la fase de aterrizaje tras 20 saltos indica una baja fuerza muscular en los miembros inferiores para mantener la actividad. Se recomienda una velocidad de 80 saltos por minuto controlada por la música en un entorno lúdico para los niños. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

Epidemiological characteristics of pulmonary tuberculosis among students in Wuhan from 2011 to 2020 / 中国学校卫生

Objective@#To analyze the epidemiological characteristics of pulmonary tuberculosis among students in Wuhan from 2011 to 2020, and to provide a basis for the scientific development of interventions and strategies.@*Methods@#Epidemiological distribution, time of onset and treatment, as well as treatment outcomes of student tuberculosis in Wuhan from 2011 to 2020 registered in the national tuberculosis information management system were described.@*Results@#During 2011-2020, 4 337 student tuberculosis patients in Wuhan were registered. The average annual incidence rate was 22.44/10 million, and the annual decreasing rate of 7.86% The incidence of male and female patients was 1.76:1, and the incidence rate of male was higher than that of female( χ 2=184.18, P <0.01). Most of patients aged 19-22 years old, accounting for 47.89%; Tuberculosis reports were highest during March to May, and September to November, and lowest during January to February, and July to August. Student patients were mainly concentrated in Hongshan District, Jiangxia District and Wuchang District, where schools were more distributed in Hongshan District, Dongxihu District, Wuchang District and Xinzhou District. The median duration from tuberculosis onset to treatment was 9(3, 21) days, which varied significantly by region, age, nationality, and patient residence ( Z =-9.25, 47.14, 9.88,43.96, P <0.01). The treatment and outcome of student tuberculosis patients were varied significantly by year and nationality( P <0.05).@*Conclusion@#The incidence of student tuberculosis in Wuhan City showed a slow downward trend. Most of student tuberculosis are college and high school students. Time and place of case detection are relatively fixed. The time of treatment and the outcome of treatment vary significantly. Tuberculosis prevention and control strategies should be formulated according to the local conditions according to the tuberculosis distribution characteristics, as well as enhancing surveillance, health promotion, active discovery and supervision management of tuberculosis in school settings.

Association between Internet gaming disorder and sleep quality among junior middle school student of Xi an City / 中国学校卫生

Objective@#To investigate the association of Internet gaming disorder (IGD) and sleep quality in adolescents of Xi an, thereby providing theoretical evidence for prevention of IGD and improvement of sleep quality of adolescents.@*Methods@#A total of 1 181 adolescents from 3 middle schools of Xi an were randomly selected between August, 2019 and February, 2020. These adolescents were assessed by a series of questionnaires, including basic information questionnaire, IGD and Insomnia Severity Index (ISI). Univariate analysis and multivariate Logistic regression model were used to evaluate the association between IGD and insomnia.@*Results@#Among 929 junior middle school students who participated in online games and the IGD Diagnostic Questionnaire was filled out in the past 12 months, the prevalence of IGD was 20.0%(186). Univariate analyses indicated that gender,whether single family, whether they living with their parents, whether they were addicted to online games, whether they could control the time of online games, and the severity of insomnia influenced IGD ( χ 2=17.11, 8.33, 202.92, 91.23, 29.06, P <0.05). The multivariate Logistic regression of the total population showed that participating in online games was not associated with the severity of insomnia ( OR = 1.62 , 95% CI =0.92-0.85, P >0.05). The people who participated in online games in the past 12 monthsthe severity of insomnia was positively correlated with the risk of IGD ( OR =3.56，95% CI =1.92-6.61, P <0.01).@*Conclusion@#Internet gaming disorder become a severe situation in the middle school students. The severity of insomnia might become the risk factor of IGD, so social should pay more attention to the prevention of internet addiction.

Association between Internet gaming disorder and sleep quality among junior middle school student of Xi an City / 中国学校卫生

Objective@#To investigate the association of Internet gaming disorder (IGD) and sleep quality in adolescents of Xi an, thereby providing theoretical evidence for prevention of IGD and improvement of sleep quality of adolescents.@*Methods@#A total of 1 181 adolescents from 3 middle schools of Xi an were randomly selected between August, 2019 and February, 2020. These adolescents were assessed by a series of questionnaires, including basic information questionnaire, IGD and Insomnia Severity Index (ISI). Univariate analysis and multivariate Logistic regression model were used to evaluate the association between IGD and insomnia.@*Results@#Among 929 junior middle school students who participated in online games and the IGD Diagnostic Questionnaire was filled out in the past 12 months, the prevalence of IGD was 20.0%(186). Univariate analyses indicated that gender,whether single family, whether they living with their parents, whether they were addicted to online games, whether they could control the time of online games, and the severity of insomnia influenced IGD ( χ 2=17.11, 8.33, 202.92, 91.23, 29.06, P <0.05). The multivariate Logistic regression of the total population showed that participating in online games was not associated with the severity of insomnia ( OR = 1.62 , 95% CI =0.92-0.85, P >0.05). The people who participated in online games in the past 12 monthsthe severity of insomnia was positively correlated with the risk of IGD ( OR =3.56，95% CI =1.92-6.61, P <0.01).@*Conclusion@#Internet gaming disorder become a severe situation in the middle school students. The severity of insomnia might become the risk factor of IGD, so social should pay more attention to the prevention of internet addiction.

Malformation of Wharton′s duct with multiple sialoliths: a case report and literature review / 口腔疾病防治

Objective@#To explore the diagnosis and treatment of ductal malformations of the submandibular gland with multiple stones.@*Methods@# A case of a malformation of Wharton′s duct with multiple sialoliths according to the clinical data, diagnosis and treatment of the patient was analyzed retrospectively.@*Results@#The patient′s physical examination and CBCT showed a tumor on the left floor of the mouth. In this case, it was found that the mass was a malformation of Wharton′s duct with multiple sialoliths according to operative exploration. The postoperative pathological examination showed (left submaxillary) salivation gland tissue, duct dilation and duct epithelia hyperplasia, duct calculus, and a large number of lymphocytes proliferating around the duct; 1 month after the follow-up, the patient had healed well. Through literature review and analysis, it was found that cases of submandibular ductal malformation with multiple stones were rare and should be carefully differentiated from arteriovenous malformation at the base of the mouth. Calculi of the submandibular gland can be removed by incision through the oral submandibular duct or by combined resection of the submandibular gland and ductal calculi, and some smaller calculi can also be treated by endoscopy of the salivary gland. @* Conclusion@# In cases of submandibular ductal malformation with multiple stones, intraoral and extraoral incisions should be performed simultaneously to remove the associated ductal stones.

Microbial concentration and particle size distribution characteristics of indoor air in colleges and universities in winter / 中国学校卫生

Objective@#To understand the microbial pollution of indoor air in colleges and universities, and to provide a reference for controlling microbial pollution and improving indoor air quality.@*Methods@#In November 2018, the air microorganisms in different functional areas and at different times were sampled and cultured in a university in Beijing by Anderson’s six-level sampler.@*Results@#During the test, there were differences in microbial aerosol concentrations in different functional areas and at different times(F=3.99，7.77, P<0.05), the concentration of bacteria in toilets was relatively high, the average concentration was 659 CFU/m3 and the concentration of fungi in the campus was relatively high, the average concentration was 660 CFU/m3; the concentration of bacteria and fungi in the air at 12 o’clock noon was relatively low, the average concentration was 320 CFU/m3 and 322 CFU/m3. The particle size distribution of microorganisms in the air presents roughly the same law, and the peak concentration of microbial concentrations occurs at the Ⅳ and Ⅴ levels (1.1-3.3 μm), in which the highest concentration of bacteria (253 CFU/m3) appears at the Ⅴ level, and the highest concentration of fungi (249 CFU/m3) appears at the Ⅳ level.@*Conclusion@#The microbial concentration in the air of colleges and universities was mainly concentrated in the size of 1.1-3.3 μm, and it was necessary to take targeted comprehensive control measures to effectively improve air quality.

Effect of IL-34 on malignant biological behavior of human acute monocytic leukemia cells / 中国肿瘤生物治疗杂志

@#[Abstract] Objective： ：To study the effects of IL-34 over-expression on malignant biological behavior of acute monocytic leukemia (AMoL) cells. Methods: The lentiviral vector pCDH-GFP for over-expressing IL-34 was constructed and infected into AMoL cell lines (THP1 and MOLM-13). Then its effects on proliferation, colony forming and cell cycle as well as apoptosis were tested by the MTS, colony formation assay and Annexin-V/PI staining, respectively. The cell differentiation phenotypes were assessed by fflow cytometry. Nude mice xenograft model was established to observe the tumor size and mass as well as the macrophages recruitment. Results: qPCR analysis showed that the expression of IL-34 mRNAin THP1-IL-34 and MOLM-13-IL-34 cells was nearly 4 000 and 3 000 folds higher than their respective control cells (all P<0.01), indicating that AMoL cell lines over-expressing IL-34 were successfully established. In vitro study showed that over-expression of IL-34 in AMoL cell lines promoted their proliferation potential(72 h:[0.738±0.003] vs [0.646±0.008]; [0.290±0.004] vs [0.247±0.004]; all P<0.01) and colony formation ([127.00 ± 3.37] vs [86.00±4.08]; [160.70±4.70] vs [116.70±3.93]; all P<0.01), whereas had little effect on apoptosis (all P>0.05). Over-expression of IL-34 promotedAMoLcell differentiation towards monocyte-macrophage lineage as the expressions of the monocyte-macrophage markers, CD11b and CD14, were increased whereas the expression of immature marker, CD71, was decreased in AMoL cell lines over-expressing IL-34(all P<0.05). Nude mice xenograft model showed that IL-34 over-expression stimulated macrophage recruitment in tumor tissues (P<0.01). Conclusion: Over-expression of IL-34 in human AMoL cell lines promotes their proliferation, colony forming potential and differentiation towards monocyte-macrophage lineage. Furthermore, IL-34 participates in the process of macrophages recruitment in vivo.

Effects and mechanisms of low concentration dopamine on hydrogen peroxide-induced apoptosis in cultured neonatal rat cardiomyocytes / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To study the effects of low concentration dopamine(DA) on hydrogen peroxide-induced apoptosis in cultured rat cardiomyocytes as well as the possible molecular mechanisms.</p><p><b>METHODS</b>Cultured neonatal rat cardiomyocytes were randomly divided into the following groups: control group (control), hydrogen peroxide group (H2O2), pretreated with low concentration dopamine ( DA + H2O2), dopamine receptor l(DR1) antagonist group (DR1 + DA + H2O2), dopamine receptor 2(DR2) antagonist group (DR2 + DA + H2O2). The cell apoptosis was then assessed by MTT and flow cytometry. The cellular ultrastructure changes were observed by transmission electron micro- scope. The activity of lactate dehydrogenase(LDH )and superoxide dismutase (SOD) in cell medium was analyzed by colorimetry. The protein expressions of Cytochrone c, Caspase 3 and Caspase 9 were obtained by Western blot.</p><p><b>RESULTS</b>Compared with hydrogen peroxide group, low concentration dopamine(10 µmol/L) decreased the apoptosis rate and the expression of protein of apoptosis related protein, enhanced SOD activity, decreased LDH activity. DR1 antagonist SCH-23390 treatment inhibited dopamine induced cardiac protective effect. DR2 antagonist haloperido treatment had no changes compared with dopamine group.</p><p><b>CONCLUSION</b>Above findings indicate that low concentration dopanine inhibits apoptosis induced by hydrogen peroxide in neonatal rat cardiomyocytes, which is partly associated with the activation of DR1.</p>

In vitro antagonistic growth effects of Lactobacillus fermentum and Lactobacillus salivarius and their fermentative broth on periodontal pathogens

As lactobacilli possess an antagonistic growth property, these bacteria may be beneficial as bioprotective agents for infection control. However, whether the antagonistic growth effects are attributed to the lactobacilli themselves or their fermentative broth remains unclear. The antagonistic growth effects of Lactobacillus salivarius and Lactobacillus fermentum as well as their fermentative broth were thus tested using both disc agar diffusion test and broth dilution method, and their effects on periodontal pathogens, including Streptococcus mutans, Streptococcus sanguis, and Porphyromonas gingivalisin vitro at different concentrations and for different time periods were also compared. Both Lactobacillus salivarius and Lactobacillus fermentum and their concentrated fermentative broth were shown to inhibit significantly the growth of Streptococcus mutans, Streptococcus sanguis, and Porphyromonas gingivalis, althoughdifferent inhibitory effects were observed for different pathogens. The higher the counts of lactobacilli and the higher the folds of concentrated fermentative broth, the stronger the inhibitory effects are observed. The inhibitory effect is demonstrated to be dose-dependent. Moreover, for the lactobacilli themselves, Lactobacillus fermentum showed stronger inhibitory effects than Lactobacillus salivarius. However, the fermentative broth of Lactobacillus fermentum showed weaker inhibitory effects than that of Lactobacillus salivarius. These data suggested that lactobacilli and their fermentative broth exhibit antagonistic growth activity, and consumption of probiotics or their broth containing lactobacilli may benefit oral health.

Neonatal intramuscular injection of plasmid encoding glucagon-like peptide-1 affects anxiety behaviour and expression of the hippocampal glucocorticoid receptor in adolescent rats.

Early-life endocrine intervention may programme hippocampal glucocorticoid receptor (GR) expression and cause psychiatric disorders in later life. Glucagon-like peptide-1 (GLP-1) has been implicated in the regulation of neuroendocrine and behavioural responses, but it is yet to be determined whether and how neonatal GLP-1 overexpression may modify hippocampal GR expression and thus programme adolescent behaviour in rats. Two-dayold pups were injected intramuscularly with vacant plasmid (VP) or plasmid DNA encoding secretory GLP-1 (GP). Anxiety-related behaviour was assessed in the elevated plus maze (EPM) test at 8 weeks of age. Plasma corticosterone levels were measured with enzyme immunoassay (EIA). Protein and mRNA levels were determined by western blot and real-time polymerase chain reaction (PCR), respectively. The DNA methylation status of the GR exon 17 promoter was determined by bisulphate sequencing PCR (BSP). GP rats exhibited anxiolytic behaviour compared with their VP counterparts. Hippocampal GLP-1 receptor (GLP-1R) and GR mRNA expression were signifi cantly elevated in GP rats without a signifi cant difference in plasma corticosterone. Signifi cant reduction in DNA methyltransferase 1 (DNMT1) expression was observed in GP rats disconnected with alterations in DNA methylation of the GR exon 17 promoter. Nevertheless, mRNA expression of nerve growth factor-inducible protein A (NGFI-A) was signifi cantly elevated in GP rats. These results suggest that neonatal intramuscular injection of plasmid DNA encoding GLP-1 affects anxiety behaviour in adolescent rats, probably through NGFI-A-activated upregulation of hippocampal GR expression.

Células de Cajal na bexiga urinária de ratos portadores de anomalia anorretal / Cajal cells in the bladder of rats with anorectal malformations

Objetivo: Avaliar o número de células de Cajal na bexiga de ratosportadores de anomalia anorretal induzida pela etilenotiouréia.Métodos: Foram utilizados 31 fetos de ratas Wistar grávidas. No110 dia de gestação foi administrada etilenotiouréia na dose de 125 mg/kg, diluída em água na concentração de 1%, por gavagem gástrica.Os fetos foram retirados por cesariana no 210 dia de gestação. Osfetos foram divididos em três grupos: G1: bexiga urinária de fetosnormais (mães não receberam etilenotiouréia), G2: bexiga urinária defetos que apresentavam anomalia anorretal sem manifestar alteraçãourológica estrutural, G3: bexiga urinária de fetos que apresentavamanomalia anorretal associada à alteração urológica estrutural. Essasbexigas urinárias foram processadas pela técnica da estreptavidinabiotina-peroxidase para pesquisa de células de Cajal avaliadas emduas localizações diferentes: suburotélio e detrussor. Resultados:Foram encontradas diferenças estatisticamente significativas entre osgrupos G1, G2 e G3 quanto à média de número de células intersticiaisde Cajal nas localizações detrussor (p < 0,001) e suburotélio (p <0,001). Em ambas as localizações, a média do número de células nogrupo G3 > G2 > G1. Conclusão: As células de Cajal estão presentesem número aumentado nos fetos portadores de anomalia anorretal,em especial quando há associação com malformações urológicas.O reconhecimento de anormalidades na morfologia e distribuição dascélulas de Cajal pode nos levar ao entendimento das alterações demotilidade em várias doenças urológicas congênitas e adquiridas.

Glycine reduces tissue lipid peroxidation in hypoxia-reoxygenation-induced necrotizing enterocolitis in rats

OBJETIVO: Avaliar o efeito protetor da glicina, num modelo experimental de ECN. MÉTODOS: Foram utilizados 50 ratos Wistar recém-nascidos, com peso variando de 4 a 6 gramas, provenientes da ninhada de seis ratas. Cinco animais foram canibalizados e, os 45 restantes, foram distribuídos em três grupos: controle G1(n=12); G2(n=16), animais que foram submetidos à hipóxia-reoxigenação; G3(n=17), animais submetidos à hipóxia-reoxigenação após uma infusão intraperitoneal de glicina 5%. Os animais foram submetidos à hipóxia em uma câmara de CO2 recebendo um fluxo de ar contendo 100% de CO2, durante 5 minutos e à reoxigenação recebendo um fluxo de O2 a 100% por 5 minutos. Segmentos de intestino delgado e cólon de 1 cm de extensão foram preparados para análise histológica. O restante do intestino foi removido em bloco e congelado a menos 80°C para homogeneização e dosagem de malondialdeído tecidual (MDA). Classificou-se as lesões teciduais de Grau 0 a Grau 5, de acordo com a extensão da lesão mucosa. RESULTADOS: Os animais do Grupo G1 apresentaram graus de lesão de intestino delgado e cólon significantemente menores do que os animais dos Grupos G2 e G3. O grupo G2 apresentou valores médios de MDA significantemente maiores do que os animais do grupo G1 (p = .015) e G3 (p=0.021). Os animais dos grupos G1 e G3 apresentaram valores de MDA que não diferiram de forma significante (p = 0.992). CONCLUSÃO: A glicina diminuiu os níveis de MDA intestinais (um marcador da peroxidação lipídica) em ratos neonatais submetidos à hipóxia-reoxigenação.

Avaliação de um modelo experimental de enterocolite necrosante neonatal em ratos / Evaluation of an experimental model of necrotizing enterocolitis in rats

OBJETIVO: Avaliar um modelo experimental de enterocolite necrosante em ratos proposto por Okur e colaboradores em 1995. MÉTODOS: Utilizou-se 28 ratos da raça EPM-Wistar no primeiro dia de vida, com peso entre 4 a 6 gramas. Os animais foram submetidos a hipóxia (H) colocando os filhotes em uma câmara de gás CO2 para sacrifício de roedores onde receberam um fluxo de ar contendo 100 por cento de CO2 durante 5 minutos. Após a hipóxia os animais foram reanimados (R) com fluxo de ar contendo O2 a 100 por cento, também durante 5 minutos. Os animais divididos em dois grupos: G1: controle (n=12): ratos não submetidos a H-R; G2: (n=16): ratos submetidos a H-R. Segmentos de intestino delgado e cólon foram preparados para análise histológica. O restante do intestino foi utilizado para dosagem de malondialdeído tecidual. RESULTADOS: Dosagem de malondialdeído do G1 foi em média 1,05 (0,44-2,03) e do G2 foi em média 2,60 (0,59- 6,4) nmol MDA/mg proteína. O G2 teve média significativamente maior do que a do grupo controle (p<0,002). Foi encontrada diferença estatisticamente significante entre os grupos de estudo quanto à distribuição do grau de lesão onde o grupo G1 apresentou graus significantemente menores do que o grupo G2. CONCLUSÕES: O modelo mostrou que a hipóxia neonatal em ratos provoca lesões na parede intestinal.. Apesar das lesões histológicas discretas é um bom método para avaliação da liberação de radicais livres teciduais.

Meningite meningococica em idoso relato de um caso / Meningococcal meningitidis in elderly case report

O artigo descreve um caso de uma mulher de 82 anos que deu entrada em nosso serviço com quadro de confusäo mental de inicio súbito, disartria e astenia intensa, associada ainda à rigidez de nuca e moderado aumento dos níveis pressóricos. Inicialmente atribuímos tal sintomatologia à doença cerebrovascular de etiologia hemorrágica, permanecendo os quadros metabólicos e infeccciosos, como diagnósticos diferenciais. Realizada análise do líquor sendo observado um padräo compatível com meningite bacteriana (isolado Neisseria Meningitidis na cultura - infrequente nesta faixa etária). Paciente evoluiu com meningococcemia, apresentando padräo hemodinâmico compatível com choque distributivo sendo necessário uso de drogas vasoativas e monitorizaçäo invasiva com recuperaçäo completa dos padrões hemodinâmicos e do quadro clínico em geral. Porém após alguns dias, apresentou aumento das escórias nitrogenadas sendo submetida a hemodiafiltraçäo veno-venosa evoluindo inicialmente bem mas com posterior aparecimento de arritmias ventrículares e óbito. A inclusäo da Doença Meningocócica no diagnóstico diferencial dos quadros confusionais do paciente idoso, apesar de infrequente, deve ser sempre lembrada, pois quanto mais precoce o diagóstico e a instituiçäo do tratamento, melhor a evoluçäo do paciente

Response of HIV/AIDS patients to Hepatitis B recombinant vaccine

Studies have demonstrated that HIV infection negatively affects the immune response to hepatitis B vacine. The present study evaluated the seroconversions to the recombinant vaccine against hepatitis B applied in HIV patients. Twenty-two patients were included in the study group all with confirmed HIV infection and with negative serum markers to hepatitis B. The control group was composed of 18 healthy individuals with negative markers for hepatitis B. All subjects were vaccinated with 20µg of ENGERIX B at 0,1 and 6 months (3 doses). The antibody response was quantitatively assessed 1 month after the third dose of recombinant vaccine. CD4 T lymphocyte counts were also performed in those beginning vaccination. Of 22 patients in the study group, only 10 (45.5 percent) responded to vaccination with protective levels (over 10 µlU/ml). In the control group, all of the subjects responded (p=0.005). Seventeen patients in the study group had their CD4 lymphocytes measured. The results suggested a direct relationship between the level of CD4 lymphocyte counts and response to the vaccine. The rate of response to hepatite B recombinant vaccine with 3 doses of 20µg of HBsAg in patients infected by the human immunodeficiency virus was significantly lower than in the control group. Patients with low CD4 T lymphocyte counts are likely to have an inadequate response to the current method of hepatitis B vaccination.