RESUMO
Three-dimensional poly (epsilon-caprolactone)/silk sericin (PCL/SS) porous nanofibrous scaffolds were prepared by electrospinning. The structure and properties of the scaffolds were characterized by Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Fourier Transform Infrared Spectroscopy (FTIR) and water contact angle instrument. Studies on cell adhension and proliferation were carried out by culturing human primary skin fibroblast cells (FEK4) on these scaffolds using SEM and MTS. The experimental results showed that the PCL/SS nanofibrous scaffolds with SS nanoparticles had porous non-woven mesh structure with nanofibrous cross-linked with each other. Fiber diameter was very uniform and precise, and the secondary structure of SS protein had not been changed. Furthermore, the capability of hydrophile increased with the SS addition, which improved FEK4 cells adhesion and proliferation on the scaffolds.
Assuntos
Materiais Biocompatíveis , Química , Adesão Celular , Células Cultivadas , Fibroblastos , Biologia Celular , Microscopia Eletrônica , Nanofibras , Química , Poliésteres , Química , Sericinas , Química , Seda , Química , Espectroscopia de Infravermelho com Transformada de Fourier , Alicerces Teciduais , QuímicaRESUMO
Although the mechanism by which migratory trophoblasts reach the spiral arteries is currently obscure, yet the process has been noted to involve the attachment, adhesion and migration of trophoblasts on the blood vessel walls. To test this, micropipette and flow chamber were used to measure quantitatively the adhesion forces and migration of early gestation human trophoblast cells (TCs) cultured on the glass slides coated with type I rat collagen or cultured with human umbilical vein endothelial cells (HUVECs). The results showed that the interdiction of integrin beta1 interaction remarkably reduced the adhesion forces of TCs to type I rat collagen or endothelial cells, and remarkably resisted the displacement of TCs induced by shear stress. By contact between TCs and endothelial cells, the TCs' adhesion force and TCs' resistance to shear stress were significantly enhanced. The results indicated that the contacts of TCs with endothelial cells enhanced the adhesion forces of human TCs, and regulated the migration of human TCs by shear stress.