RESUMO
Objective@#To analyze the level of immunoglobulin E (IgE) changes with benzene exposure workers.@*Methods@#Firstly, through occupational health monitoring, 68 hospitalized cases were discovered who were suspected chronic benzene poisoning. Secondly, according to the GBZ68-2013《The diagnosis of occupational benzene poisoning》standard diagnosis and indexing, 68 cases were divided into the benzene poisoning group (n= 29) and the benzene exposure group (n=39) . 50 cases of healthy workers without benzene exposure were for the control group. Use the immune luminescence method to detect IgE levels. Thirdly, Case-control study was used, observing IgE changes though the three groups by statistical analysis.@*Results@#Compared with control group, the level of leukocyte、neutrophil and IgE was drop in benzene exposure group with statistically significant (P<0.05) . Compared with benzene exposure group, IgE of benzene poisoning group was rise, with statistically significant (P<0.05) , IgE of mild benzene poisoning group rise the most obvious, with statistically significant (P<0.05) . Compared with benzene exposure group, IgE of moderate benzene poisoning group was drop, without statistically significant (P>0.05) .@*Conclusion@#Benzene occupational exposure can induce immunosuppression, IgE decreases, and reduces immune surveillance. The response of the IgE level in the mild benzene poisoning patients was significantly elevated, whether it is protective response of the body immune function needs to be studied further investigated.
RESUMO
Objective To explore the correlation between HBV-DNA and serum HBV markers quantitative detection and determine its diagnosis value in hepatitis B virus infection. Methods The HBV-DNA was measured by fluorescence quantitative PCR and hepatitis B virus was detected by time-resolved fluorescence immunoassay (TRFIA). The relationship between the two tests was analyzed statistically using software. Results Detection of HBV-DNA in the peripheral blood of patients with HBsAg (+), HBeAg (+), HBcAb ( + ) showed 92. 9% (79/85) positive rate,and the average DNA copy number was (4.31 ± 1.64) × 106 copies/ml. While the detection of HBV-DNA in the peripheral blood of patients with HBsAg ( + ), HBeAb ( + ), HBcAb ( + ) showed 47.7% (105/220) positive rate, and the average DNA copy number was (2.47 ± 2. 21 ) × 104 copies/ml. In normal controls,blood HBV-DNA detection was negative. Furthermore, HBV-DNA copy number correlated positively with HbeAg quantitation (r = 0. 59, P = 0. 041 ). However, we found no significant correlation between HBV-DNA copy number and HbsAg quantititation (r = 0.221, P = 0.077). Conclusions Different HBV-M had diverse correlation with HBV-DNA number, comprehensive detection of them would be better to assist clinical diagnosis and evaluate the response to treatments.