Pagetoid reticulosis positive for cytotoxic CD20 and CD30: A case report

Pagetoid reticulosis is an indolent cutaneous T-cell lymphoma and presents as erythema or plaque with a well-defined border on the distal areas of the extremities. Immunophenotypic studies show that in most cases, neoplastic lymphocytes are positive for CD4, whereas CD20 and CD30 double positivity was rarely reported. In this paper, we report an 80-year-old woman who presented with erythema on the extremities for 3 years. Skin biopsy on the right forearm was performed. Histopathologically, the erythematous lesions were characterized by atypical lymphocytes with significant epidermotropism. Immunohistochemical staining showed high proliferation as evidenced by high Ki-67 index and that the tumor cells were positive for CD20 and CD30 but negative for CD7 and CD56. The patient was treated with one cycle of radiotherapy and is currently doing well.

environmental deposition of influenza virus from patients infected with influenza A [H1N1] pdm09: Implications for infection prevention and control

In a multi-center, prospective, observational study over two influenza seasons, we sought to quantify and correlate the amount of virus recovered from the nares of infected subjects with that recovered from their immediate environment in community and hospital settings. We recorded the symptoms of adults and children with A [H1N1] pdm09 infection, took nasal swabs, and sampled touched surfaces and room air. Forty-two infected subjects were followed up. The mean duration of virus shedding was 6.2 days by PCR [Polymerase Chain Reaction] and 4.2 days by culture. Surface swabs were collected from 39 settings; 16 [41%] subject locations were contaminated with virus. Overall, 33 of the 671 [4.9%] surface swabs were PCR positive for influenza, of which two [0.3%] yielded viable virus. On illness Day 3, subjects yielding positive surface samples had significantly higher nasal viral loads [geometric mean ratio 25.7; 95% Cl 1.75, 376.0, p = 0.021] and a positive correlation [r = 0.47, ' p = 0.006] was observed between subject nasal viral loads and viral loads recovered from the surfaces around them. Room air was sampled in the vicinity of 12 subjects, and PCR positive samples were obtained for five [42%] samples. Influenza virus shed by infected subjects did not detectably contaminate the vast majority of surfaces sampled. We question the relative importance of the indirect contact transmission of influenza via surfaces, though our data support the existence of super-spreaders via this route. The air sampling results add to the accumulating evidence that supports the potential for droplet nuclei [aerosol] transmission of influenza