Analysis on genetic characteristics of mumps virus strains circulating in Hunan province in 2011 / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To be acquainted with genetic characteristics and variation of mumps virus strains circulating in Hunan province.</p><p><b>METHODS</b>Mumps virus (MV) strains were isolated using Vero/ SLAM cells. The small hydrophobic protein (SH) genes of MV isolates were sequenced, and the sequences were analysed phylogenetically between the isolated strains and other reference mumps strains.</p><p><b>RESULTS</b>4 mumps virus strains were isolated from 16 specimens collected in 2011 from different regions of Hunan province. The genotype of isolated strains were supposed to be F type.</p><p><b>CONCLUSION</b>Genotype F is the main genotype of circulating strains in Hunan province in 2011 and there is no variation between genotype.</p>

Clinical Characteristics and Immune Function Change of Cytomegalovirus Infection in Newborn Infants and Little Infants / 实用儿科临床杂志

Objective To explore the clinical manifestations,diagnosis and treatment,restoration of disease and immune function change of pediatric patients infected by cytomegalovirus (CMV). Methods The clinical manifestations of 52 CMV patients:15 cases (29%) of common infections,12 cases (23%) of hepatitis,11cases (21%) of inflammatory reactions,6 cases (12%) of pneumonia,4 cases (7%) of rash,2 cases (4%) of CMV encephalitis and 2 cases of (4%) intrauterine growth restriction. The examination of peripheral blood,including leucocyte counts and classification and platelet counts,were performed twice,6 ?L/time. C-reaction protein (CRP) using femoral vein were tested right after admission and during treatment period. The molecular biology technique was used to detect pathogens. Liver function examination was also performed. Moreover,the examination of cell immune function was done using the flow cytometer to test lymphocyte counts.Results 1.Blood test:white blood counts of 2 cases (4%) exceeded 20?109 L-1,and 18 cases (35%) in the area of (10-20)?109 L-1,the other patients were all normal.The lymphocyte counts in 46 cases (88%) increased and the thrombocyte counts in 12 cases (23%) were less than 100?109 L-1,the lymphocyte and thrombocyte counts of other patients were all normal.2.CRP:11 cases (21%) exceeded 20 mg/L,others were