Association between pulse pressure and new-onset diabetes in hypertensive patients / 中华心血管病杂志

Objective: To determine the association between pulse pressure and the risk of new-onset diabetes in hypertensive patients. Methods: In this prospective cohort study, hypertensive patients from the Kailuan Study, who were diagnosed in 2006-2007 check-up, were screened for enrollment. Participants who finished the biennial follow-up until December 31, 2017 were finally included in this analysis. The primary outcome was incident diabetes development. The pulse pressure variables were divided into quartiles (Q1-Q4), and the Kaplan-Meier curve was used to examine and estimate the cumulative incidence of new-onset diabetes among quartiles. Cox proportional hazards regression model was performed to explore the association between pulse pressure and the risk of new-onset diabetes in hypertensive patients. Results: During an average follow-up of 8.17 years, 6 617 new-onset diabetes were identified out of the 32 917 hypertensive patients with no history or evidence of diabetes in 2006-2007 check-up. Participants were classified into quartiles according to pulse pressure levels as follows: Q1 group(<41 mmHg (1mmHg=0.133kPa))(n=7 995); Q2 group(41-<51 mmHg) (n=8 196); Q3 group (51-<61 mmHg) (n= 8 270); Q4 group (≥61 mmHg) (n=8 456). The cumulative incidences of new-onset diabetes across the quartiles were 16.94%, 19.61%, 21.07%, and 22.33%, respectively, with the incidence density was 20.27, 23.20, 24.92, and 26.10 per 1 000 person-years, respectively. The cumulative incidence of new-onset diabetes increased in proportion with increasing pulse pressure levels (P<0.01 by the Log-rank test). After multivariate adjustment, compared with the first quartile, the hazard ratios for new-onset diabetes in the third and fourth quartiles were 1.13 (95%CI 1.04-1.22, P<0.01) and 1.14 (95%CI 1.05-1.24, P<0.01), respectively. The risk of new-onset diabetes increased 5%(HR=1.05, 95%CI 1.02-1.08, P<0.01) with the fractional pulse pressure increased per 1 SD (0.13). Findings from the three sensitivity analyses were consistent with the main results in this cohort. Conclusions: Pulse pressure at baseline is positively associated with the incidence of new-onset diabetes among hypertensive individuals, and pulse pressure is an independent risk factor for the development of diabetes in hypertensive patients.

A novel vitrified cryopreservation method for the establishment of tumor tissue biobank / 中国组织工程研究

BACKGROUND: Vitrified cryopreservation is a novel method for specimen preservation, which has a potential application value in the establishment of tumor biopsy biobank. OBJECTIVE: To investigate the effectiveness of vitrified cryopreservation and the feasibility in the establishment of tumor biopsy biobank. METHODS: The fresh biopsy specimens of liver metastasis from rectal cancer were randomized into vitrified cryopreservation and control groups. Then, the biopsy specimens from each group were implanted subcutaneously into the mouse back to establish the humanized mouse xenograft model. The biological characteristics and histological changes of tumor tissues in each group were detected by Calcein-AM/Hoechst33342 staining, as well as immunohistochemistry and hematoxylin-eosin staining. Totally 105 biopsy tissues of liver metastasis from rectal cancer were preliminarily collected and cryopreserved to establish the tumor biopsy biobank. RESULTS AND CONCLUSION: No significant differences in biological viability or histological features of tumor biopsy was detected before and after cryopreservation (P > 0.05), suggesting that vitrified cryopreservation can effectively preserve tumor biopsy and is favorable for the establishment of tumor biopsy biobank.

Studies on diversity of quality of Forsythia suspensa / 中国中药杂志

<p><b>OBJECTIVE</b>To study on diversity of quality of Forsythia suspense collected from different regions.</p><p><b>METHOD</b>The hundred-seed weight of shucks was analyzed by the method of hundred grain mass. The thousand-seed weight of seeds was analyzed by the method of thousand grain mass. The contents of the active components in shucks and seeds were determined by HPLC.</p><p><b>RESULT</b>The hundred-seed weight of shucks, the thousand-seed weight of seeds and the contents of the active components in the shucks and seeds from different regions were significantly different.</p><p><b>CONCLUSION</b>The quality of F. suspense from different regions is not consistant.</p>

Study on quantified relationship between contents of active components in Forsythia suspense and nutrition of soil / 中国中药杂志

<p><b>OBJECTIVE</b>To sift the main effective factors of active component of Forsythia suspense.</p><p><b>METHOD</b>The active component of F. suspense was mensurated by HPLC, and the contents of nutrition in soil was determined by conventional methods. The active component of F. suspense and the nutrition of soil collected from different regions was analyzed by SPSS 11.0 for windows software package. Accounted the partial correlation coefficients of soil factor to the active component of F. suspense, and established regression equations.</p><p><b>RESULT</b>Soil available phosphorus is the most important factor that effected forsythiaside content in the seeds of F. suspense, followed by kalium, pH, FACT; Forsythin in the seeds of F. suspense is mainly effected by soil abailable kalium and FACT; pH is the important factor effected Forsythinin in F. suspense, followed by soil available phosphorus, soil available kalium, and FACT; and the main effectivefactors of forsythiaside in F. suspense are soil available phosphorus, soil available kalium.</p><p><b>CONCLUSION</b>The contents of nutrition in soil is one of important factors which affect the active component of F. suspense.</p>