Effect of cisplatin on apoptosis of spiral ganglion cell and expression of caspase-3 in mouse cochlea / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To establish a mice model of cisplatin-induced ototoxicity, and to investigate the effect of cisplatin on apoptosis of spiral ganglion cell and expression of caspase-3 in mouse cochlea.</p><p><b>METHODS</b>Terminal deoxynucleotidyl transferase-mediated nick end labeling method (TUNEL) was used to monitor the apoptosis of spiral ganglion cell. Envision method of immunohistochemistry was applied to detect the expression of caspase-3 in cochlea. Auditory brainstem response (ABR) was measured to observe the change of hearing.</p><p><b>RESULTS</b>The weight and hearing of mice in different dose of cisplatin groups were declined significantly as compared with those of control group (P < 0.05, P < 0.01), and the TUNEL positive cell number and expression of caspase-3 were greater remarkably with the more cisplatin injected.</p><p><b>CONCLUSION</b>A mouse model of cisplatin-induced ototoxicity can be established. Cisplatin can lead to the apoptosis of spiral ganglion cells, and caspase-3 has participated in this apoptosis process, which approves further that apoptosis might be one of the mechanisms of cisplatin ototoxicity.</p>

Multiple central clinical studies on the needling method for regulating wei and strengthening brain for treatment of insomnia / 中国针灸

<p><b>OBJECTIVE</b>To observe the clinical therapeutic effect of the needling method for regulating wei and strengthening brain on insomnia.</p><p><b>METHODS</b>Two hundred cases of insomnia were randomly divided into a test group and a control group, 100 cases in each group. The test group were treated with the needling method for regulating wei and strengthening brain with Baihui (GV 20), Dazhui (GV 14), Shenmai (BL 62), Zhaohai (KI 6) and ear points Yuanzhong, Shenmen selected; and in the control group, Sishencong (EX-HN 1), Shenmen (HT 7) and Sanyinjiao (SP 6) were selected. Acupuncture was given once daily for 15 days. Pittsburgh sleep quality index (PSQI) was used for scoring before and after treatment.</p><p><b>RESULTS</b>The total effective rate was 89.0% in the test group and 65.0% in the control group with a very significant difference between the two groups (P<0.01); the difference of PSQI scores before and after was -9.15+/-5.68 in the test group and -5.64+/-5.73 in the control group, with a very significant difference before and after treatment in the two groups (P<0.01).</p><p><b>CONCLUSION</b>The therapeutic effect of the needling method for regulating wei and strengthening brain on insomnia is better than that of normal needling method.</p>