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OBJECTIVE: To investigate the proportion of 8 kinds of ginsenoside to ginsenoside Rg1 in Panax ginseng and the regularity of growth year in Jilin province, and to provide reference for the identification of growth year. METHODS: The samples of garden ginseng, wild-cultivated ginseng and wild ginseng were collected from different growth years (3-30 years) in Jilin province. The contents of 8 ginsenoside (ginsenoside Rg1, Re, Rf, Rb1, Rc, Rb2, Rb3, Rd) in P. ginseng were determined by HPLC. The contents of saponins as well as the proportion of 8 kinds of ginsenoside to ginsenoside Rg1 were calculated; the relationship of the proportion with growth year was investigated. RESULTS: As the increase of growth year, the proportion of 8 kinds of ginsenosides in garden ginseng to ginsenoside Rg1 as well as that of ginsenoside Re, Rb1, Rc, Rd to ginsenoside Rg1 were decreased gradally (P<0.001); the proportion of ginsenoside Re to ginsenoside Rg1 in wild-cultivated ginseng decreased first and then increased(P<0.001); the proportion of 8 kinds of ginsenosides to ginsenoside Rg1 as well as the proportion of ginsenoside Re and Rb1 to ginsenoside Rg1 were increased gradually in wild ginseng (P<0.001); the proportion of ginsenoside Rf, Rb3 to ginsenoside Rg1 in garden ginseng, wild-cultivated ginseng and wild ginseng had no significant difference(P>0.05). CONCLUSIONS: Garden ginseng, wild-cultivated ginseng and wild ginseng contain 8 kinds of ginsenosides. The growth year can be predicted preliminarily according to the proportion of ginsenoside Rg1 and ginsenoside Re, Rb1 to ginsenoside Rg1.
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Experimcnts showed that the nest of Macrotermes annandalei (Silvestri ) had the activity of relieving co gh, eliminating phlegm, and bacteriostasis. It coild also promote the phagocytosis of macrophages, enhance the transformation of T-cell of lymphocyte and rasiethe ratio of ANAE cell of lymphocyte in mice.
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AIM: To screen macroreticular resins for isolation and purification of flavonoids from the leaves of Diospyros kaki Linn (FLDK). METHODS: 8 kinds of macroreticular resins were assayed for their adsorbability and deadsorbability as well as the adsorption kinetics to FLDK. RESULTS: Polyamide and AB-8 resin were found with good adsorbability and deadsorbability as well as adsorption kinetics to FLDK. CONCLUSION: AB-8 resin and polyamide could be used for preparing high contents of FLDK. FLDK-P70, a flavonoid extract contained more than 77% of flavonoids was prepared from the extract of leaves of Diospyros kaki by polyamide adsorption column isolation.