Determination of Main Components in Idrocilamide Ointment by HPLC / 中国药房

OBJECTIVE:To establish an HPLC method to determine the content of main component in idrocilamide ointment. METHODS: The samples were separated on HP-ODS column with column temperature maintained at room temperature. The mobile phase consisted of methanol-water (65∶35) with a flow rate of 1.0 mL?min-1. The detection wavelength was set at 276 nm and the injection volume was 20 ?L. RESULTS: The linear range of idrocilamide was 0.05～4 ?g?mL-1,the average recovery rate was 99.97% (RSD=0.80%). CONCLUSION: This method is sensitive,accurate and applicable for the determination of the content of main component in idrocilamide ointment.

Optimization of the Prescription of Traditional Chinese Medicine for MS by Uniform Design / 中国药房

OBJECTIVE: To optimize the prescription of traditional Chinese medicine for motion sickness (MS).METHODS: Zingiber officinale,Herba pogostemonis,Aucklandia lappa were extracted respectively,and rotating-inducing MS mice were enrolled in uniform design.The prescription was optimized with MS index as the parameters.RESULTS: The optimal prescription was as follows:60 g Z.officinale,45 g Herba pogostemonis,5 g A.lappa.The extractive of prescription was significantly better than dimenhydrinate in the treatment of MS.CONCLUSION: MS index is a stable and sensitive parameters and it is suitable for screening and evaluation of anti-MS drugs.R.zingiberis,H.pogostemonis,A.lappq are potential drug for MS.

Determination of the Serum Concentration of Buspirone by HPLC in Rabbits / 中国药房

OBJECTIVE:To establish a HPLC method for determination of the concentration of Buspirone in serum.MET_ HODS:The chromatographic system consisted of ZORBA SB-C 18 column and mobile phase of methanol-acetonitrile-water-0.1mol/L NaAC buffer solution(pH4.5)(10∶38∶51∶1),with a detection wavelength of237nm,and the content was calcu?lated by peak area internal standard method.RESULTS:The linear range of Buspirone was1～80ng/ml,r=0.9979.CONCL_ USION:This method is simple and accurate for determination of Buspirone in serum.

Study on in Vitro Percutaneous Permeability of Methylphenidate Cream / 中国药房

OBJECTIVE: To study in vitro percutaneous permeability of methylphenidate cream. METHODS: Isolated rat skin was taken as permeable barrier. The influence of different concentrations of azone(0%, 2%. 5% ) in methylphenidate cream on drug permeation was observed. RESULTS: Steady-state percutaneous flow(J ) of methylphenidate cream with 2% and 5% azone increased 27. 80% and 49. 05%. respectively. CONCLUSION: Methylphenidate cream will be a safe. effective and conve- nient new preparation.

Experimental Study of Percutaneous Absorption of Neostigmine Cream in Vitro / 中国药房

OBJECTIVE:To study the in vitro percutaneous absorption of neostigmine cream.METHODS:Using isolated mouse skin as permeation barrier,the influence of different concentrations of azone on percutaneous permeation of neostigmine was studied.RESULTS:Azone could obviously enhance the percutaneous permeation of neostigmine,and the accumulative permeation quantity of neostigmine cream containing2%and5%azone increased7.17%and11.87%respectively compared with that of cream without azone.CONCLUSION:The percutaneous permeability of neostigmine cream is satisfactory and the best concentration of azone in enhancing permeation seems to be5%.

Study on in Vitro Percutaneous Absorption of Galanthamine Cream / 中国药房

OBJECTIVE:To study the in vitro percutaneous permeability of galanthamine cream.METHODS:Using iso?lated mouse's skin as barrier to permeation,the promoting effect of azone in different concentrations on permeation of galanth_ amine was studied.RESULTS:Azone could obviously enhence the permeability of galanthamine through skin.The steady flux of galanthamine cream containing2%and5%azone increased56.12%and23.29%respectively.CONCLUSION:Galanthamine cream has good percutaneous permeability and2%azone promotes the permeability best.