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Objective To investigate the expression of(glutathione S-Transferase Omega-1,GSTO1)in cervical cancer tissue and its correlation with patient survival time,and to explore the impact of GSTO1 N-glycosylation on proliferation,migration,invasion,and epithelial-mesenchymal transition of cervical cancer.Methods By using immunohistochemistry,the expression levels of GSTO1 in tumor cells of 82 cervical cancer patients were detected,and the correlation between GSTO1 expression and clinical pathological features was analyzed.Kaplan-Meier meth-od was used to plot survival curves and evaluate the impact of GSTO1 expression in cervical cancer tissues on pa-tient survival time.Univariate and multivariate Cox regression analyses were performed to assess the independent prognostic factors influencing cervical cancer prognosis.The NetNGlyc 1.0 Server database predicted potential N-glycosylation modification sites of GSTO1(Asn55,Asnl35,Asn190).The cervical cancer cells(HeLa)were transfected with GSTO1 N-glycosylation site mutation vectors at positions 55,135,and 190,as well as GSTO1 wild-type vector and empty vector.Stable transfected cells were selected using puromycin.Western blot experi-ments were performed to assess the effectiveness of lentiviral interference.The effects of GSTO1 N-glycosylation site mutations on proliferation,migration,and invasion of HeLa cells were evaluated using EdU proliferation assay,wound healing assay,and Transwell assay.The effect of GSTO1 N-glycosylation site mutations on the epithelial-mesenchymal transition of HeLa cells was detected using the Western blot experiment.Results Immunohistochem-istry results revealed high expression of GSTO1 in cervical cancer tissues.The expression rate of GSTO1 was signifi-cantly higher in cervical cancer tissues with deep stromal invision≥1/2,lymphovascular space invasion,and lymph node metastasis(P＜0.05).Moreover,high expression of GSTO1 was associated with poorer overall surviv-al.After N-glycosylation site-specific mutation of GSTO1,the cell count of proliferation,migration,and invasion in HeLa cells significantly decreased(P＜0.05).The Western blot results showed that N-glycosylation site mutation of GSTO1 significantly inhibited the epithelial-mesenchymal transition of HeLa cells.Conclusion The expression of GSTO1 in cervical cancer tissues is associated with stromal infiltration depth,lymphovascular space invasion and lymph node metastasis,and it is also correlated with shorter patient survival time.Site-specific mutations in GSTO1 N-glycosylation significantly inhibit the proliferation,migration and epitheli al-mesenchymal transition of HeLa cells.
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Objective:To study the effect of minimally invasive surgery training on the complications of laparoscopic radical surgery for early cervical cancer.Methods:72 cases of continuous surgery were retrospectively included. According to the learning curve and order of the chief surgeon, they were divided into two learning stages (the first stage and the second stage). The clinicopathological characteristics and surgical complications of the two stage group were compared.Results:The proportion of patients with previous abdominal surgery in the second stage group was higher (55.6% vs 31.1%, P=0.041), and the proportion of patients with local advanced clinical stage was also higher (29.6% vs 6.7%, P=0.022). There was no significant difference in the pathological characteristics between the two groups (all P>0.05). In all 72 cases of laparoscopic radical surgery, there were 4 cases of serious surgical injury (3 cases in the first stage group and 1 case in the second stage group), and 36 cases of postoperative complications (20 cases in the first stage group and 16 cases in the second stage group). There was no significant difference in the incidence of serious surgical injury and surgical complications between the two stage groups ( P=1.000, 0.224). Multivariate logistic regression analysis showed that age >51 years old was an independent risk factor for surgical complications after laparoscopic radical resection of early cervical cancer ( HR=3.404, 95% CI: 1.132-10.234, P=0.029). Conclusions:The incidence of complications in laparoscopic radical hysterectomy of cervical cancer for trained operators is low, and the impact of surgical learning curve on complications is small, but its impact on serious surgical injury cannot be ruled out.