HPLC Fingerprint of Radix Gentianae / 中草药

Objective To establish the HPLC fingerprint of methanol-resoluble component in Radix Gentianae collected from different habitats and accordingly offer the evidence for quality control and quality evaluation of Radix Gentianae.Methods Ten batches of Radix Gentianae were measured by the RP-HPLC,and their fingerprints were obtained.Chromatographic condition: ODS-column,gradient elution with methanol-water,the detection wavelength was at 254 nm,flow rate was at 1.0 mL/min,and the column temperature was 25 ℃.Results The mutual mode of Radix Gentianae was set up and then the similarity was compared with software which was published by the Committee of Pharmacopeia of China.Conclusion According to the analysis of statistics,this method is steady,accurate with good repeatability,and can be used as standardization implantation and quality control of Radix Gentianae.

Establishment of fingerprint of processing Gentianae scraba Bge and its mutual mode by HPLC / 中成药

AIM:To establish the HPLC fingerprint of methanol-resoluble component of processing Gentiana scraba Bge collected from different habits to provide evidence for quality control and quality evaluation of Gentiana scraba Bge.METHODS:Ten batches of processing Gentiana scraba Bge were measured by RP-HPLC,and the mutual mode was made by the similarity degree evaluating system 2004 of Chromatographic Fingerprint of Traditional Chinese Medicine promulgated by Chromatographic condition,consisted of Agilent-Eclipse-XDB-C_ 18-column and gradient elution with methanol-water,the detection wavelength was at 254 nm,flow rate was at 1.0 mL/min and the column temperature was at 20 ℃.RESULTS:The mutual mode of processing Gentiana scraba Bge was set up and the similarity of fingerprints of processing Gentiana scraba Bge from different habits was gained.CONCLUSION:According to the analysis of statistics,this method is steady,accurate with good repeatability and can be used as standardization implantation and quality control of Gentiana scraba Bge.

Swertiamarin of related overground and underground organ of Gentiana / 中成药

flower, changed with the blossoming period. CONCLUSION: It may be a relationship between the content and blossoming period.