The predictive value of preoperative fibrinogen level in peripheral blood combined with platelet-to-lymphocyte ratio in the prognosis of colorectal cancer / 医学研究生学报

ObjectiveThe local recurrence or distant metastasis of colorectal cancer (CRC) after surgical resection still directly affects the prognosis of CRC patients.This study aims to investigate the clinical significance of preoperative peripheral blood fibrinogen (Fbg) level combined with platelet-to-lymphocyte ratio (PLR) in the prognosis of CRC.MethodsThe clinicopathological characteristics of 108 CRC patients who were diagnosed by pathology and underwent surgical resection in Wujin hospital affiliated to Jiangsu University from January 2013 to December 2015 were analyzed. Fbg and PLR thresholds were determined by ROC curve, and the patients were grouped according to Fbg combined with PLR (F-PLR) to analyze the relationship between different F-PLR scores and the clinicopathological characteristics and prognosis of CRC patients.ResultsPLR showed statistically significant difference in the left and right half of the tumor (P0.05). Multivariate analysis using COX regression showed that F-PLR score, TNM stage and lymph node metastasis were independent risk factors for the prognosis of CRC patients (P<0.05).ConclusionThe F-PLR score (Fbg and PLR combined) can be used as a predictor of treatment effect and prognosis of CRC patients to guide clinical treatment decisions and prognoses.

Effect of gap size between tooth and restorative materials on microbiolism based caries in vitro / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the effect of gap size between tooth and restorative materials on microbiolism based caries in vitro.</p><p><b>METHODS</b>Tooth blocks made of human molars without caries and the same size composite resin blocks were selected and prepared. Tooth-resin matrix was mounted on resin base with a gap size of 0, 25, 50, 100, 190, 250 µm and a control group was dealed with adhesive system. Six experimental groups and one control group were included, with 8 samples in one group and a total of 56 samples. The samples were cultured by a 14-day sequential batch culture technique. The development of outer surface lesion and wall lesion was assessed with confocal laser scanning microscope (CLSM) by measuring the maximum lesion depth, fluorescence areas and average fluorescence value. The data were collected and statistically analyzed. The deposits of the tooth-restoration interface and the development of the carious lesion were observed by scanning electron microscope (SEM).</p><p><b>RESULTS</b>Most groups showed outer surface lesion and wall surface lesions observed by CLSM and SEM except 2 samples in control group. There was no significant difference on the outer surface lesion (P > 0.05). The maximum lesion depth [(1145.37 ± 198.98), (1190.12 ± 290.80) µm respectively], the maximum lesion length, fluorescence areas and average fluorescence value of 190 and 250 µm groups' wall lesions were significantly higher than the 0, 25, 50 and 100 µm groups [the maximum lesion depth was (205.25 ± 122.61), (303.87 ± 118.80), (437.75 ± 154.88), (602.87 ± 269.13) µm respectively], P < 0.01. With the increase of the gap size, the demineralization developed more seriously. While the maximum lesion depth, the maximum lesion length and fluorescence areas of 0, 25, 50 µm groups' wall lesions were of no significant difference.</p><p><b>CONCLUSIONS</b>There was close relationship between gap size and wall lesion when the gap was above 100 µm at tooth-composite resin interface. The existence of gap was the main influencing factor on the development of microbiolism based caries lesion.</p>

Relationship of gallstone formation after radical gastrectomy with the polymorphisms of apolipoprotein B Xba I and lipoprotein lipase Hind III gene / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To investigate the relationship of gallstone formation after radical gastrectomy with the polymorphisms of apolipoprotein B (ApoB) Xba I gene and lipoprotein lipase (LPL) Hind III gene.</p><p><b>METHODS</b>A total of 80 gastric cancer patients who underwent radical gastrectomy at our hospital between January 2006 and December 2006 were divided into different groups according to the polymorphisms of ApoB Xba I gene and LPL Hind III gene. The gene polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism. Gallstone formation 2 years after radical gastrectomy was compared among different genotype groups.</p><p><b>RESULTS</b>Eight patients were lost to follow-up. According to the genotype detection, 72 patients were divided into X(+)X(-) group (10 cases), X(-)X(-) group (62 cases), H(-) group (27 cases) and H(-) deletion group (45 cases). The incidence of gallstone was significantly higher in X(+)X(-) group than that in X(-)X(-) group (60.0% vs 6.5%, P<0.01). The serum levels of total cholesterol TC and low density lipoprotein were significantly higher in X(+)X(-) group than those in X(-)X(-) group (P<0.05), but the level of ApoB was not significantly different between the two groups. The incidence of gallstone was not significantly different between H(-) group and H(-) deletion group (14.8% vs 13.3%). The level of triglyceride in H(-) group was significantly lower than that in H(-) deletion group before operation, however the difference disappeared after operation.</p><p><b>CONCLUSION</b>X(+) allele may be associated with gallstone formation after radical gastrectomy, while H(-) may not.</p>

Expression of RhD antigen on RBC of different RhD serotype by flow cytometry / 中国实验血液学杂志

This study was purposed to establish the method of quantifying RhD antigen on red blood cells (RBC) by flow cytometry (FCM) and to explore the expression of D antigen on RBC of different RhD serotype. RhD(+) RBCs and RhD(-) RBCs were mixed in 1:1 ratio. Cells were stained by the indirect method (IgG anti-D as the first antibody, FITC-anti-IgG F(ab')2 as the second antibody), and the ratio of RhD(+) on RBCs was quantified by FCM. The optimal dosage of IgG anti-D was defined. Expression of RhD antigen on RBC of RhD(+), weak D, RhDel and RhD(-) type were detected by FCM. The results showed that optimal dilution of IgG anti-D monoclonal antibody was 1:4, 1x10(6) cells/50 microl. The percentage of D(+) RBC of RhD(+), weak D, RhDel and RhD(-) type were 96.8+/-2.97%, 79.5+/-9.88%, 47.8+/-11.43%, 3.7+/-2.96%, respectively. The mean fluorescence intensity (MFI) of RhD antigen expression of RhD(+), weak D, RhDel and RhD(-) type were 33.3+/-6.21 Dal, 18.6+/-5.39 Dal, 7.10+/-1.17 Dal, 0.79+/-0.55 Dal, respectively. In conclusion, there are significant differences of RhD antigen expressions among RBC of different RhD serotypes. The level of antigen on RhD(+) RBC is the highest and then weak D the next, while the level of antigen on RhDel RBC is the lowest level.