AngioJet in patients treated for acute deep venous thrombosis by mechanical aspiration thrombectomy combined with catheter-directed thrombolysis / 中华普通外科杂志

Objective To evaluate AngioJet in patients treated for acute deep venous thrombosis (DVT) by mechanical aspiration thrombectomy combined with catheter-directed thrombolysis (CDT).Methods A retrospective analysis of 83 acute DVT patients treated by mechanical aspiration thrombectomy combined with CDT from Dec 2014 to Oct 2018 in the Third Hospital of Hebei Medical University was conducted.Results 43 cases (routine group) were treated by mechanical aspiration thrombectomy using a large-caliber catheter combined with CDT,and 40 cases (AngioJet group) were treated by AngioJet aspiration thrombectomy combined with CDT(P ＞ 0.05).Thrombosis was completely dissolved in 28 cases in AngioJet group while in 10 cases in large-caliber catheter group.Venous damage rate was higher (38 cases vs.13 cases) in routine group.Conclusion AngioJet combined with CDT can effectively and safety remove thrombus in the lower extremity deep venous system.

Design of self-evaluation scale on basic ability in graduates of preventive medicine major and its reliability and validity test / 中华医学教育探索杂志

Objective@#To design the self-rating scale on basic ability in graduates of preventive medicine major, and test its reliability and validity, providing a reliable tool for evaluating the basic ability of graduates.@*Methods@#On the basis of constructing the Evaluation Index System on Basic Ability in Graduates of Preventive Medicine Major, the three-level indexes were converted to specific questions. Self-evaluation scale including 58 items, 22 sub-dimensions and 8 main dimensions were finally designed after pre-survey and expert argumentation. A total of 205 graduates of preventive medicine major from a medical college and 9 experts in the field of public health were selected to completed the questionnaire. Scale's reliability was tested by applying internal consistency reliability and composite reliability; it's validity was tested by applying content validity and confirmatory factor analysis.@*Results@#Scale's α coefficient of internal consistency reliability was 0.976; α coefficient and combination reliability of eight main dimensions were both higher than 0.8; the content validity index S-CVI of the scale was 0.94, the content validity index I-CVI of the item level was 0.78 to 1.00; the average variance extraction amount (AVE) of the eight main dimensions was higher than 0.50. Fitting of the structural model with the item used as observation indexes was reasonable (χ2/df=1.749, RMR=0.036, RMSEA=0.061, NFI=0.801, IFI=0.904, TLI=0.904, CFI=0.903), and the sub-dimension as used observation indexes was good(χ2/df=1.876, RMR=0.032, RMSEA=0.066, NFI=0.913, IFI=0.958, TLI=0.945, CFI=0.957).@*Conclusion@#This scale is the first self-rating scale on the basic ability in graduates of preventive medicine major on the basis of the National Standards of Teaching Quality on Public Health and Preventive Medicine, with good reliability and validityl. It is able to achieve quantitative evaluation on graduates' basic abilities.

Design of self-evaluation scale on basic ability in graduates of preventive medicine major and its reliability and validity test / 中华医学教育探索杂志

Objective To design the self-rating scale on basic ability in graduates of preventive medicine major, and test its reliability and validity, providing a reliable tool for evaluating the basic ability of graduates. Methods On the basis of constructing the Evaluation Index System on Basic Ability in Graduates of Preventive Medicine Major, the three-level indexes were converted to specific questions. Self-evaluation scale including 58 items, 22 sub-dimensions and 8 main dimensions were finally designed after pre-survey and expert argumentation. A total of 205 graduates of preventive medicine major from a medical college and 9 experts in the field of public health were selected to completed the questionnaire. Scale's reliability was tested by applying internal consistency reliability and composite reliability; it's validity was tested by applying content validity and confirmatory factor analysis. Results Scale's αcoefficient of internal consistency reliability was 0.976; α coefficient and combination reliability of eight main dimensions were both higher than 0.8; the content validity index S-CVI of the scale was 0.94, the content validity index I-CVI of the item level was 0.78 to 1.00;the average variance extraction amount (AVE) of the eight main dimensions was higher than 0.50. Fitting of the structural model with the item used as observation indexes was reasonable ( x2/df=1.749, RMR=0.036, RMSEA=0.061, NFI=0.801, IFI=0.904, TLI=0.904, CFI=0.903), and the sub-dimension as used observation indexes was good(x2/df=1.876, RMR=0.032, RMSEA=0.066, NFI=0.913, IFI=0.958, TLI=0.945, CFI=0.957). Conclusion This scale is the first self-rating scale on the basic ability in graduates of preventive medicine major on the basis of the National Standards of Teaching Quality on Public Health and Preventive Medicine, with good reliability and validityl. It is able to achieve quantitative evaluation on graduates' basic abilities.

Research status and development tendency of unmanned aerial vehicles for casualty evacuation / 医疗卫生装备

Objective To explore the application of unmanned aerial vehicles (UAVs) technology for casualty evacuation in disaster rescue.Methods The research status of casualty evacuation using unmanned aerial vehicles was described in the disaster medical rescue,and several UAVs involved in casualty evacuation were introduced.According to different dynamic structures,casualty evacuation UAVs were divided into four types,such as traditional rotor helicopter,multi-rotor aircraft,ducted fan aircraft,compound aircraft.Meanwhile,the load capacity and flight speed of involved UAVs were analyzed.Results The advantages of casualty evacuation UAVs in the previous researches related were summarized,with the problems and technical difficulty discussed.Furthermore,the development tendencies of casualty evacuation UAVs were predicted.Conclusion Casualty evacuation UAVs can transport the wounded to the professional medical institutions outside of the disaster scene instead of rescue crews,to reduce the casualty rate of rescuers and avoid secondary damage of the injured personnel,with accurate point to point casualty evacuation.

Grasp torque control based on experiential learning and haptic feedback / 医疗卫生装备

Objective To propose a grasp torque control based on experimental learning and haptic feedback to facilitate the manipulator in dexterous manipulation.Methods An experience database was built firstly,and then the object was recognized by tactile feedback in the grasp task.If the object had been experitentially grasped,the torque was output based on the database.In case a new object was grasped,the optimal output torque was calculated by iterative learning.Results The experiment showed that the robot hand could find the experiential output torque quickly when encountering the object in database and calculate the torque by iterative learning to achieve grasp task.Conclusion The experiential database grows up when the robot hand learns more and more experience.It can fast output torque like human in the grasp task.

Analysis of HCMV gN genotypes in patients with HCMV-HIV co-infection / 中华微生物学和免疫学杂志

Objective To analyze the distribution of various genotypes of human cytomegalovirus glycoprotein N ( HCMV gN) in patients with HIV infection; to investigate the effects of HCMV-HIV co-in-fection on disease progression and the relationships between HCMV gN genotypes and disease progression. Methods Patients with active HCMV infection were screened out from 359 patients with HIV infection by using the pp65 antigenemia assay.The genes encoding HCMV gN ( UL73 ) were amplified by nested PCR ( nPCR) .The amplicons were digested by restriction enzymes including MboⅠ, ScaⅠ and SalⅠ.Then, the restricted fragment length polymorphisms were further analyzed on 4%agarose gel.The relationships be-tween HCMV genotypes and the morbidity and mortality of acquired immune deficiency syndrome ( AIDS ) were investigated via a prospective study.Results Among the 359 patients with HIV infection, 28 subjects were positive for the HCMV pp65 antigenemia assay.The HCMV gN genotypes in 20 patients with active HCMV infection were distributed as: gN-3a (4/20, 20%), gN-1 (4/20, 20%), gN-4d (1/20, 5%), gN-4b (1/20, 5%) and mixed infection (10/20, 50%).Patients with HCMV-HIV co-infection were more likely to develop AIDS during the follow-up period (RR=9.78).Patients harboring HCMV gN-1 and gN-4 genotypes would seem likely to have 4.6 times of chance leading to AIDS-associated death than those harbo-ring other HCMV gN genotypes.Conclusion HCMV infection ( especially gN-1 and gN-4 genotypes) might accelerate the progression of HIV infection.

Effect of bisphenol A on differentiation potential of mouse embryonic stem cells / 中国药理学与毒理学杂志

OBJECTIVE To explore the effect of bisphenol A (BPA) on the differentiation potential of embryonic stem cells, and provide an experimental basis for evaluation of safety of BPA. METHODS Mouse embryonic fibroblasts (MEFs) and embryonic stem cells (ESCs) were treated with BPA 0.1, 1, 10, 100 and 1000 μmol.L-1 for 8 d respectively. The viability of MEFs and ESCs was measured by CCK-8 and lC50 was calculated. The mRNA expression of α-myosin heavy chain in ESCs was tested by RT-PCR to determine lD50 . The embryonic body cultured by suspension method was treated with BPA 0.001, 0.01, 0.1 and 1 μmol.L-1 for 10 d respectively. The changes of marked genes in each blastoderm were detected by RT-PCR. RESULTS lC50 of BPA to mouse ESCs was 5.22×10-4 mol.L-1 , and to MEFs was 6. 25 × 10-4 mol.L-1 . lD50 of BPA to mouse ESCs differentiating to cardiomyocytes was 7.0×10-7 mol.L-1 . BPA 0.001 and 0.01 μmol.L-1 upregulated the expression of the marked genes of mesoderm, fetal liver kinase-1 and globin transcription factor 1. CONCLUSION BPA is a strong embry-otoxic compound. BPA of low concentration can promote the differentiation of mouse ESCs to mesoderm.

Unconserved C terminal of pUL76 in human ctomegalovirus elicits aggresome formation / 安徽医科大学学报

Objective To define the nuclear agrresome formation is determinated by which part of the UL76 of HCMV. Full-length, conserved N terminal and unconserved C terminal of pUL76 were constructed to eukaryotic ex-pression plasmid pEGFP-N1 . Methods Primers were designed to amplify full-length and different part of pUL76 according to standard sequence of HCMV AD169 which had been submitted to GenBank(FJ527563. 1). These frag-ments were constructed to eukaryotic expression plasmid pEGFP-N1 . The recombinant plasmids were designated pEGFP-UL76,pEGFP-UL76N, pEGFP-UL76C respectively. Double digestion and sequencing were performed to verify the accuracy of recombinant plasmids construction. Empty vector and three recombinant plasmids were transi-ent transfected to HELF and HepG-2 cells respectively. Reverse transcriptation PCR and Western blot were per-formed to detect the RNA and protein expression level respectively. Different nuclear aggresome formations were visualized with an Olympus fluorescence microscopy. Results pEGFP-N1 and pEGFP-UL76N were unable to in-duce nuclear aggresome formation, whereas pEGFP-UL76 and pEGFP-UL76C were able to elicit nuclear aggresome formation. Conclusion The unconserved C terminal of pUL76 is sufficient to induce nuclear aggresome formation.

Development of pyrogenation treatment vehicle of medical wastes / 医疗卫生装备

Objective To develop a medical wastes pyrolysis vehicle for field hospital, countryside hospital and public emergencies.Methods Configuration design, thermodynamics theory, ergonomics and auto control technology were involved in to manufacture open-type compartment, on-board pyrolysis furnace, smoke control system, and pyrolysis furnace control and monitoring system.Results The vehicle passed 7 000 km reliability test, high-low temperature test, smoke emission test and army trials.Conclusion The vehicle behaves well in performances and environment protection, and gains high economic and social benefits.

Design of on-board medical waste pyrolysis furnace / 医疗卫生装备

Objective To design an on-vehicle medical waste pyrolysis furnace for the safe disposal of medical wastes, which could disposal 1t wastes each day. Methods A furnace was designed by in-depth research of pyrolysis, calculation analysis in detail, and taking the experimental results and other factors in. The flue gas treatment system of this medical waste pyrolysis furnace was designed through 3T principle.Results This furnace behaved well in configuration, reliability and self support, whose dioxin emission was limited as 0.213 ng/m3.Conclusion The on-vehicle medical waste pyrolysis furnace gains advantages in safety and environment protection, and has a brilliant future in practice.

Design of an anesthesia and micro-environment information management system in mobile operating room / 生物医学工程学杂志

We have designed a mobile operating room information management system. The system is composed of a client and a server. A client, consisting of a PC, medical equipments, PLC and sensors, provides the acquisition and processing of anesthesia and micro-environment data. A server is a powerful computer that stores the data of the system. The client gathers the medical device data by using the C/S mode, and analyzes the obtained HL7 messages through the class library call. The client collects the micro-environment information with PLC, and finishes the data reading with the OPC technology. Experiment results showed that the designed system could manage the patient anesthesia and micro-environment information well, and improve the efficiency of the doctors' works and the digital level of the mobile operating room.

Design of Pyrolyzing Furnace Temperature Measurement Node Based on WSN Technology / 医疗卫生装备

Objective To propose a design of pyrolyzing furnace temperature measurement node based on WSN technology. Methods The chip MAX6675 was applied in the temperature measure circuit in the system. It simplified the design of hardware and software and improves precision of control system. The chip CC2420 was adopted as the frequency transceiver. The operating system TinyOS which was developed by the University of California at Berkeley was also adopted. Results We accomplished the design of temperature data acquisition module,wireless communication module and power management module,which had many features,such as simple structure,small size and high precision. Conclusion The node has effectively improved the functional reliability of pyrolyzing furnace,giving a solid base to promote the application of WSN technology on medical vehicle.

Application and Prospect of Wireless Net Technology in Medical Equipment / 医疗卫生装备

Objective To apply wireless net technology in our military's medical equipment more effectually. Methods The author analyzed the existing wireless net technology and its domain of application to find out the application actuality of wireless net in our military's medical equipment. Results The wireless net technology is widely used in the medical equipment and has good application prospects. Conclusion The author will continue to deepen the application research in wireless network technology in hope to improve the level of informatized military medical equipment.

A Prevalent Cohort Study on Occupational Stress and Hypertension / 中国心理卫生杂志

Objective:To identify the influence of occupational stress on occurrence of hypertension.Methods:A prevalent cohort of 964 male workers employed for at least 3 years with different occupational stress were investigated with Occupational Stress Index, hypertension was diagnosed by WHO criteria of 1996. Results:The occupational stress was a risk factor for hypertension with RR=1.70 and in a dose-effect pattern after adjusted for other factors. In high exposure group, the adjusted incidence density was 12.47/per 1000 person-years with RR=2.99, in the medium exposure group, that was 8.81/per 1000 person-years with RR=2.11, that of low exposure group was 4.17/per 1000 person-years, the differences between different groups had statistic significance. When the length of exposure was more than 10 years, the adjusted incidence of hypertension increased significantly in high and medium exposure groups.Conclusions:Causality between occupational stress and hypertension exist, but it needs long exposure of at least 10 years for occurrence of hypertension.

Prostatic Hyperplasia and Sexual Hormones Level Change in Serum Induced by Nonylphenol in Rats / 环境与健康杂志

0.05).The body weight and the increase of body weight in 100 mg /kg NP group was significantly lower than those in control group(P

Research and Development on Digital Temperature Recorder System of Vehicle-mounted Blood Bank Based on ATmega48 / 医疗卫生装备

Objective To research and develop a new type of temperature recorder system of vehicle-mounted blood bank which is used in the field training and combat. Methods Based on ATmega48 SCM as control core, the components including digital temperature recorder, LCD, buzzer and so on are used. Results The recorder can not only monitor temperature and display real-time, but also store the data in the recorder of the serial EEPROM (CAT24C64). Additionally, when the temperature exceeds the given range, it can be automatic alarm. Conclusion The temperature recorder with a short collection cycle, the record for a long time and high sensitivity, cost-effective, small size and easy to carry, etc., can be widely used in medicine, food, chemicals, electronic manufacturing, warehousing, logistics, as well as agriculture and other sectors.

Application of CAN-bus in Medical Vehicle / 医疗卫生装备

Objective Nowadays,with the development of medical vehicle,there are many parameters for inspection in its interior.It has disadvantages,such as complex routing and low system reliability,to use DCS.CAN bus technology can decrease routing and enhance system reliability.Methods OPC technology was used.A temperature supervisor control program and a database application were designed by VB,and the temperature was control by the fuzzy control methods.Results The temperature supervisor control system was established with CAN bus to explore the application of CAN bus in the medical vehicle.Conclusion It can enhance the system's reliability to use CAN bus technology in the medical vehicle.