Relationship between serum homocysteine level and oxidative stress in carotid artery atherosclerosis patients with essential hypertension / 中国基层医药

Objective To detect the levels of carotid intima-media thickness(CAIMT),serum homocysteine(HCY),malondialdehyde(MDA) and superoxide dismutase(SOD) activity,in order to investigate the correlation between carotid atherosclerosis(CAS) and serum HCY and oxidative stress in essential hypertension(EH) patients.Methods 50 patients with EH and 266 healthy controls were enrolled in this study.The color Doppler ultrasonography was used to detect CAIMT,and circulating enzyme method was used to detect serum HCY,and MDA levels were detected by TBA and SOD activity was detected by colorimetric assay of and the results of the examination were statistically analyzed.Results The incidence rate of CAS in EH patients was 62.8%(167/266),associated with age,disease and related course,and there was no significant difference in gender.In EH concurrent CAS group,the serum HCY level,MDA level and SOD activity were (28.06±6.94)μmol/L,(13.10±2.75)μmol/L and (69.73±6.51)U/mL,respectively,which of the control group were (11.74±3.62)μmol/L,(4.71±1.18)μmol/L and (136.07±10.16)U/mL,respectively,the levels of serum HCY and MDA in EH complicated CAS group were significantly higher than those in the control group(t=7.118,6.326,all P<0.05),and the activity of SOD was significantly decreased.The serum HCY level was positively correlated with MDA level(r=0.493,t=9.207,P<0.05),and negatively correlated with serum SOD activity(r=-0.562,t=11.040,P<0.05).Conclusion EH patients have high levels of HCY,MDA and SOD activity of low levels.HCY level is significantly increased and oxidative stress is increased in CAS patients.Oxidative stress is associated with serum HCY level.

Group B Streptococcus Infection in Vaginal Tract of Perinatal Pregnant Women and Drug Resistance Analysis / 现代检验医学杂志

Objective To provide evidences which are for clinicians to formulate effective prevention and treatment measures by analyzing the perinatal pregnant women vaginal group B streptococcus (GBS)infection and adverse pregnancy outcomes. Methods From January 2013 to February 2015,the vaginal secretions were sampled from 795 cases of perinatal pregnant women for culture identification of GBS,the final results were statistical analyzed.Results In the 795 pregnant women ca-ses,there were 256 GBS carriers cases,the carrier rate was 32.2%.There was statistically significance (χ2 = 19.095,P <0.01)between less than 30 years old group (28.9%)and greater than or equal to 30 years old group (42.3%).The differ-ent incidence rate of clinical symptoms between GBS negative cases and GBS positive cases (18.8% vs 8.0%)was also had statistically significance (χ2 =39.514,P <0.01).The antimicrobial resistant rates of ten kinds of antibacterial drugs (vanco-mycin,linezolid,penicillin,ampicillin,ceftriaxone,nitrofurantoin,levofloxacin,clindamycin,erythromycin and tetracycline) was 0,0,0.6%,3.1%,6.6%,9.6%,21.9%,23.8%,29.9% and 58.1% respectively.The positive rate of D-bacteriostatic ring experiment was 23.9%.Conclusion The carrier rate of perinatal pregnant women GBS was higher in this region,and the elderly were easier to be infected.Perinatal pregnant women infected with GBS to vancomycin and rina thiazole amine and penicillin and ampicillin and ceftriaxone and with nitrofurantoin because of the sensitive rate is high.

Value of non phosphorylated insulin -like growth factor binding protein -1 in cervical vaginal excretion and residual amniotic fluid index monitoring in the diagnosis of premature rupture of membranes / 中国基层医药

Objective To explore the method of rapid diagnosis preterm premature rupture of membrane (PPROM)and prognosis factors of residual amniotic fluid in mother and infant through monitoring nonphosphorylated insulin -like growth factor binding protein -1 (IGFBP -1)and the residual amniotic fluid index (AFI)of the cervi-covaginal secretions from PPROM women.Methods 96 cases of PPROM were tested IGFBP -1 of cervicovaginal secretions,AFI were tested by abdominal ultrasound through fast chromatography methods,and the cause was ana-lyzed.The pregnancy outcome and neonatal prognosis were compared.Results 92 cases positive of IGFBP -1 were diagnosed with PPROM.The risk factors of PPROMwere mainly reproductive tract infections,abortion,induced labor history and unknown causes (including no evidence of infection,fetal membrane dysplasia unclear or other factors), the twins,multiplets,macrosomia,breech or cephalopelvic disproportion,gestational hypertension disease,uterine fibroids or deformity,malnutrition and polyhydramnios.In the 92 patients with PPROM pregnant women,the duration from premature rupture of membrane to delivery was (105.7 ±13.9)h,cesarean section rate was 51.1%,fever rate of pregnant women was 7.6%,the rate of placental abruption was 4.3%,the rate of fetal distress was14.1%,perinatal infection rate was 21.7%,and neonatal asphyxia rate was 7.6%,the neonatal mortality was 7.6%.To compare the AFI≤40mm group with AFI >40 mm group,the duration from premature rupture of membrane to delivery was short-ened remarkably (t =10.904,P <0.05),the neonatal mortality rate was significantly increased (χ2 =4.359,P <0.05).Conclusion Measurement of IGFBP -1 and AFI in cervicovaginal secretions can rapid diagnosis PPROMand evaluate the results of pregnancy and neonatal prognosis,and can guide clinical improvement of pregnancy safety and reduce neonatal mortality.

Study on group B streptococci in perinatal pregnant women reproductive tract infections and adverse preg-nancy outcomes / 中国基层医药

Objective To analyze the perinatal pregnant women vaginal group B streptococcus (GBS)infec-tion and and adverse pregnancy outcomes and provide evidences which were for clinicians to formulate effective pre-vention and treatment measures.Methods From January 2013 to February 2015,the vaginal secretions were sampled from 795 cases of perinatal pregnant women for culture identification of GBS,and the clinical symptoms and adverse pregnancy outcomes were observed.The final results were statistical analyzed.Results In the 795 pregnant women, there were 256 GBS carriers,the carrier rate was 32.2%.There was statistically significance (χ2 =19.095,P <0.01)between less than 30 years old group (28.9%)and greater than or equal to 30 years old group (42.3%).The different incidence rate of clinical symptoms between GBS negative cases and GBS positive cases (18.8%,8.0%) was also had statistically significance(χ2 =39.514,P <0.01).Compared with the GBS negative groups,the different incidence rate of premature rupture of membranes,preterm delivery,intrauterine infection and neonatal infections of GBS also existed statistical significance (χ2 =29.355,10.543,31.797,13.291,all P <0.01 ).Conclusion The carrier rate of perinatal pregnant women GBS was higher in this region,and the elderly were easier to be infected.Peri-natal pregnant women infected with GBS can increase the occurrence of adverse pregnancy outcomes,and should be given clinical intervention.

Effects of preincubation medium and other factors affecting in vitro fertilization rate of post-thawed genetically engineering mouse spermatozoa / 中国比较医学杂志

Objective To discuss the effect of in vitro fertilization ( IVF) and mouse sperm cryopreservation , to establish a simple and economic frozen system for the genetically engineering mice preservation .Methods Sperm from genetically engineering mice were cryopreserved , IVF was performed using post-thawed sperm, then embryo transfer, to compare the effects of cryopreservation medium、age of male mice and sperm preincubation medium .Results Using CPA as sperm cryopreservation medium , when PM was used thawed-sperm preincubation in IVF , the fertility rates were from 82.49%to 91.43%, when HTF was used thawed-sperm preincubation in IVF , the fertility rates were from14.46%to 27.38%, there was a signification difference between PM and HTF sperm preincubation medium;10 to 35 weeks male genetically engineering mice sperm were succeed cryopreservation , and positive mice were procreated after 2-cell embryos were transferred;R18S3、CPM and CPA was used to freeze sperm , the fertility rates were 75.85%、88.89%to 94.27%, positive mice were procreated after 2-cell embryos were transferred;2-cell embryos after IVF were freezed , then thawed and positive mice were procreated after 2-cell embryos were transferred .Conclusion Using CPA as sperm cryopreservation medium , when PM was used thawed-sperm preincubation in IVF , genetically engineering mice sperm were succeed cryopreservation .

Study on the relationship of carotid artery atherosclerosis and helicobacter pylori infection in patients with essential hypertension / 中国医师杂志

Objective To study the correlation of carotid artery atherosclerosis (CAS) and helicobacter pylori (Hp) infection in patients with essential hypertension (EH) by observing the carotid intima-media thickness (CAIMT),helicobacter pylori (Hp)-IgG antibody,blood lipid,high sensitive C-reactive protein (hs-CRP),and homocysteine(HCY).Methods To measure CAIMT,blood lipid,serum Hp-IgG antibody,hs-CRP,and HCY were measured by color doppler ultrasound instrument,and Hp-IgG antibody by colloidal gold method.Results A total of 150 EH patients were randomly divided into CAIMT and Hp-IgG-antibody groups,and were taken statistical analysis.Results There were 89 patients with EH CAIMT ＞ 1.0 mm(59.3％) as the intimal thickening,and 67 cases of Hp-IgG antibody(+) (44.7％) for Hp infection.CAIMT thickening (73.1％) in the Hp infection group was significantly higher than that of Hp infection group (48.2％) (P ＜0.05).In the thickened CAIMT group,Hp infection (55.1％) was significantly higher than that of CAIMT non thickening (29.5％) (P ＜0.05).EH patients complicated with CAIMT thickening and Hp infection had significant correlation (r =0.179,t =2.213,P ＜0.05).In the Hp infection group,the levels of serum triglyceride (TG),total cholesterol(TC),low density lipoprotein cholesterol (LDL-C),HCY,and hs-CRP were significantly higher than those of Hp infection group (P ＜ 0.05).Serum high density lipoprotein cholesterol (HDL-C) were significantly lower than those of the Hp infection group (P ＜ 0.05).Conclusions The study proved the correlation of CAS and Hp infection in patients with EH,and promoted the development of CAS probably by influencing the blood lipid metabolism,inflammatory reaction and HCY levels.It would be one of the treatment of EH with CAS by removing or prevention of Hp infection.

Effects of intensive insulin therapy on IL-10 level and NF-ΚB activity in patients undergoing cardiopulmonary bypass / 中国基层医药

Objective To observe the effects of intensive insulin therapy on IL-10 level and NF-ΚB activity in peripheral blood mononuclear cells in patients undergoing cardiopulmonary bypass.Methods The non-diabetic patients undergoing cardiopulmonary bypass in our department were selected and assigned to intensive therapy group (group A,n=40) and received strict glycemic control after the initiation of surgery.And those who undergoing cardiac surgery but without strict glycemic control were assigned to routine therapy group (group B,n=40) as controls.The blood glucose in group A was maintained at 4.4～8.3mmol/L,whereas the glucose in group B was below 11.lmmol/L.The concentration of serum IL-10 and NF-ΚB activity in peripheral blood mononuclear cells was measured at different time points.Results There were no significant differences in general data between two groups.The concentration of IL-10 in group B was significantly lower than that in group A(P<0.05).compared with group B,strict glycemic control markedly suppressed NF-KB activation (P<0.05).Conclusion Intensive insulin therapy could reduce the activity of NF-ΚB and then reduce the expression of IL-10.Strict glycemic control could significantly mitigate the systemic inflammatory response.

The early diagnosis of myocardial damage in patients with severe head injuries / 国际外科学杂志

Objective Discusstheinfluence of early diagnosis of myocardial damage and heart-type fatty acid binding protein(h-FABP)on patients with severe head injury after rehabilitation.Methods The sorum h-FABP was detected using enzyme-linked immunosorbent one-step quantitative detection,cTnI using solid-phase enzyme-linked immunosorbent assay(ELISA),CK-MB using the method of immunosuppressive.Results The sorum levels of h-FABP,cTnl and CK-MB in severe head injury patients levels were significantly higher than that of healthy control group(P<0.01),the positive serum h-FABP in severe head injury patients Was significantly higher than cTnI,CK-MB or ECG(P<0.01);8 cases died in47 cases of severe head injury patients,the mortalisty rate was 17.02%(8/47),the mortality rate of abnormal sentrum h-FABP,cTnI and CK-MB group was significantly higher than the normal group(P<0. 01);The serum h-FABP in predicting the death of severe head injury patients was high in sensitivity and negative predictive value,but lower in specificity and accuracy.Condusiom The serum h-FABP quantitative assay as a high-sensitive indicators to minor myocardial dsmage can be used an objective indicators forjudging severity ofacute severe head injury,evaluating incidence of accidents.

An analysis about long term results of terminal non-cell lung cancer through chemotherapy and rediotherapy / 中国基层医药

Objective To study the long-term results of 90 cases with inoperable stage Ⅲ non-small cell lung cancer.Methods All patients received CAP regimen or MVP regimen chemotherapy.80 patients received radiotherapy.Results The results showed that 1-,3-,5-year survival rates were 53.4%,19.5% and 8.5% respectively.The 3-,5-year survival rate for squamous cell carcinoma were higher than those adenocarcinoma(P

Clinical effects on the chemotherapy of neoadjuvent chomotherapy for surgical resection in patients with stage ⅢA non-small cell lung cancer / 中国基层医药

Objective To investigate the effect of neoadjuvent chomotherapy of paclitexel plus eisplatin for improving the surgical resection rate of stage ⅢA non-small cell lung cancer(NSCLC).Methods 46 patients with stage ⅢA NSCLC were randomly divided into neoadjuvant chemotherapy group(25 caeses) and simple operative group(25 cases).Neoedjuvant chemotherapy group(NCG) received two-cycle chemotherapy and then underwent operation.Simple operative group(SOG) was only treated by operation.Results The operative resection rate of NCG was 99.0%,radical resection rate was 64.0%.It was higher obviously than that simple operative group(SOG)(P