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ObjectiveTo explore the expression of transcription factor KLF16 in nonalcoholic fatty liver disease （NAFLD） and its effect on lipid metabolism. MethodsAn animal model of NAFLD was constructed in mice induced by a high-fat diet. The mice were divided into normal diet group （ND） and high fat diet group （HFD）. NAFLD cell model was constructed by primary mouse liver cells induced by oleic acid. The cells were divided into control group （Control group） and oleic acid induction group （OA group）. Real-time fluorescence quantitative PCR （RT-qPCR） and Western blot were used to detect KLF16 expression in NAFLD animal and cell models. In vitro and in vivo models of KLF16 knockdown were constructed by injection of adeno-associated virus （AAV） into mouse tail veins and transient transfection of cell siRNA. Hematoxylin-eosin staining （HE） and other methods were used to detect changes in lipid deposition in NAFLD models before and after KLF16 knockout. RT-qPCR was used to detect the expression of key genes of lipid metabolism in both cellular and animal NAFLD models before and after KLF16 knockdown. Western blot assay was used to detect the expression of endoplasmic reticulum stress protein in NAFLD model before and after KLF16 knockdown. ResultsThe expression level of KLF16 was up-regulated in HFD group and OA group， and lipid deposition was increased in OA group after KLF16 was depressed. There was no change in TC level in hepatocytes between groups （P>0.05）， and TG level was increased in different degrees （P<0.05， P<0.001）. At the same time， the change of KLF16 expression also caused the change of ER stress protein expression in OA group. ConclusionThe transcription factor KLF16 may alleviate lipid deposition in nonalcoholic fatty liver disease by endoplasmic reticulum stress.
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Objective:To analyze the quality control test results of urine fluoride laboratories in Jiangxi Province, and support reliable data for the monitoring and prevention of fluorosis.Methods:From 2019 to 2021, the Jiangxi provincial urine fluoride laboratories used the "Determination of Fluoride in Urine-Ion Selective Electrode Method" (WS/T 89-2015) to detect quality control samples. Origin 9 software was used to analyze the data, and Youden plots and deviation bar plots were produced. The results were evaluated based on the Z-score (qualified: │Z│ ≤2, basically qualified: 2 < │Z│ < 3, unqualified: │Z│ ≥3).Results:The qualified rate of quality control assessment results of urine fluoride laboratories in Jiangxi Province was 95.00% (38/40) . Most of urine fluoride laboratories had scores of │Z│ < 2, and two laboratories had scores │Z│≥3 in 2019 and 2020.Conclusion:The quality control assessment results of urine fluoride laboratories in Jiangxi Province are relatively satisfactory, but some laboratories still need to further improve their detection capabilities.
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Objective:To analyze the results of external quality control network of iodine deficiency disorders (IDD) laboratories in Jiangxi Province in 2018, to summarize the total detection level and capability at all levels, and to provide reliable quality assurance for monitoring and prevention of IDD.Methods:In 2018, IDD laboratories of provincial, municipal and county-level in Jiangxi Province were examined for the external quality control of salt iodine, urinary iodine and water iodine. Among them, there were 1 provincial, 11 municipal and 30 county-level laboratories participated in salt iodine assessment; there were 1 provincial, 11 municipal and 100 county-level laboratories participated in urinary iodine assessment; and there were 1 provincial and 11 municipal laboratories participated in water iodine assessment. Salt iodine was determined by direct titration method (GB/T 13025.7-2012); urinary iodine was determined by As 3+-Ce 4+ catalytic spectrophotometry method (WS/T 107.1-2016) or inductively coupled plasma mass spectrometry method (WS/T 107.2-2016); water iodine was determined by the method in the range of 0 - 100 μg/L in "Study on the Method Suitable for the Detection of Water Iodine in Areas with Iodine Deficiency and High Iodine" recommended by national IDD reference laboratory. Urinary iodine was determined with Z score method, when│Z│≤ 2, it was qualified; when 2 <│Z│ < 3, it was basically qualified; when│Z│≥ 3, it was not qualified. Water iodine and salt iodine were determined by the method of reference value ± uncertainty, and the average value of the test results within this range was judged to be qualified; otherwise, it was judged to be unqualified. Results:In 2018, the feedback rates and qualified rates of salt iodine, urinary iodine and water iodine in 1 provincial and 11 municipal laboratories in Jiangxi Province were all 100.00%. Thirty county-level laboratories participated in the salt iodine assessment, and 1 of them failed. One hundred county-level laboratories participated in the urinary iodine assessment, and 3 of them failed.Conclusion:Laboratory testing technology of IDD continues to maintain at a high level in Jiangxi Province in 2018, and the detection ability of some county-level laboratories needs to be further improved.
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Objective To evaluate the control effects on coal-burning-borne endemic fluorosis in Shangli County and Yuanzhou District of Jiangxi Province in 2014.Methods Self-evaluation at the county level was carried out in all diseased villages in Shangli County and Yuanzhou District,including 145 villages of Shangli County and 32 villages of Yuanzhou District.Review at the provincial level was based on the results of selfevaluation of the county,3 towns were chosen in each county (district),3 villages were chosen in each town,a total of 18 villages were re-checked in Shangli County and Yuanzhou District,and 10 households were chosen to recheck in each village.According to the project requirements for endemic disease control,in all diseased villages of Shangli County and Yuanzhou District,self-evaluation results of the county was used to evaluate the status of the improved and the used stoves,and dental fluorosis of children aged 8-12.Review at the provincial level was carried,and the results were evaluated based on the Standards for Control Criteria for Endemic Fluorosis Areas (GB 17017-2010) and Elimination of Coal-burning-borne Endemic Fluorosis.Results Self-evaluation of the county:110 443 households in Shangli County and 20 280 households in Yuanzhou District were investigated,the qualified rates of improved stoves were 92.45％ (102 109/110 443) and 98.28％ (19 932/20 280),respectively;the correct-usage rates of qualified stoves were 93.46％ (95 427/102 109) and 98.47％ (19 627/19 932),respectively.Dental fluorosis of 28 772 children were examined,the number of dental fluorosis was 5 730,the prevalence rate of dental fluorosis was 19.92％ (5 730/28 772);Shangli County and Yuanzhou District were up to the standard of fluorosis control,the rates of control were 95.17％ (138/145) and 100.00％ (32/32),respectively.Review at the provincial level:180 households were selected,the qualified numbers of improved stoves and the correct-usage of qualified stoves were 179 and 177,respectively,the qualified rate of improved stoves was 99.44％ (179/180) and the correct-usage rates of qualified stoves was 98.88％ (177/179).Dental fluorosis of 805 children were examined,the number of dental fluorosis was 139,the prevalence rate of dental fluorosis was 17.27％ (139/805).The consistency rate of county-level and provincial-level was 88.89％ (16/18).Conclusion The control efficiency for coal-burningborne fluorides in Shangli County and Yuanzhou District of Jiangxi Province is obvious,the prevalence is under control.
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Objective To investigate the association of impaired glucose regulation and adiponectin (APN)with the clinical severity of coronary lesions.Methods A total of 210 cases of suspected coronary heart disease were examined by coronary artery angiography.The patients were differentiated as 4 groups:42 patients with normal glucose tolerance (NGT),36 patients with impaired fasting glucose(IFG),92 patients with impaired glucose tolerance (IGT;including 44 cases with postpraudial 2h plasma glucose(2 hPG) ＜ 10 mmol/L as IGT1 subgroup and 48 cases with 2h PG ≥ 10 mmol/L as IGT2 subgroup),and 40 patients with combination of IFG and IGT.Accordingly,body mass index (BMI),blood pressure,blood lipid,insulin,APN and CRP were measured to evaluate by Gensini score.Results The incidence of coronary heart disease and Gensini scores in IGT and IGT+IFG groups were significantly higher than those in either IFG or NGT subset(P＜0.05).APN in both IGT and IGT+IFG subsets was significantly lower than that in IFG or NGT subsets(P＜0.05),CRP values were significantly raised in both IGT and IGT+IFG subgroups compared with the other 2 subgroups(P＜0.05).Statistical difference in Gensini scores and APN was found between the 2 IGT subgroups (P＜0.05).Gensini scores were negatively correlated with APN level.Multivariate regression analysis showed that both APN and HOMA-IR values were independently correlated with the Gensini scores.Conclusion The lowered APN may serve as a more sensitive factor in predicting the coronary lesions in patients with IGR,especially in IGT cases.It woula be beneficial to cardiovascular complication by controlling the postprandial blood glucose level below 10 mmol/L.
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Objective To investigate the effect of total flavonoids of Herba Epimedii (HEF) on the apoptosis of osteoblasts in the ovariectomized (OVX) rats. Methods Fifty-four female rats were allocated into 6 groups (9 rats in each group) ; OVX group, sham-operated group, 3 groups with OVX followed by three different dosages of HEF(40,80 and 160 mg· kg-1·d-1) and another group by nilestriol (0.1 mg· kg-1·d-1) for 12 weeks respectively. Bone mineral density (BMD) of whole body of rats was determined by DEXA. The serum level of estradiol was measured by radioimmunologic assay. Apoptotic ceils were examined using terminal dexynudeotidyl transferase-mediated dUTP nick end labeling (TUNEL) and observed by electron microscope. The protein expressions of transforming growth factor (TGF)-β1 ,fibroblast growth factor (FGF)-2 and Fas were tested withimmunohistochemical methods. Results Compared with the OVX group,BMD in the HEF treated (80 and 160 mg ·kg-1·d-1) groups were increased (P < 0.01), but the serum level of estradiol was not increased (P >0.05). Positive expression rate of apoptotie osteoblasts and osteocytes in the OVX group was significantly higher than that in sham-operated group after 12 weeks (P < 0. 01), and were decreased after HEF treatment (P < 0.01).Compared with the OVX group, Fas expression in the HEF treated groups was significantly decreased (P < 0.01), and expressions of TGF-β1 and FGF-2 were significantly increased, especially in 80 and 160 mg·kg-1·d-1 HEF groups (P < 0.01). Conclusion HEF has antiosteoporotic effect in ovariectomized rats. HEF can inhibit apoptosis of osteoblasts and osteocytes, which may thus contribute to its antiosteoporotic effect.