RESUMO
【Objective】 To evaluate the ability of different disinfection methods to remove nucleic acid pollution in 2019-nCoV so as to obtain the best removal scheme. 【Methods】 2019-nCoV positive quality control nucleic acid of 50 μL was applied to plastic， metal and glass with medical cotton swabs， respectively. After drying， we dropped 50 μL of 750 mL/L alcohol （ethanol）， chlorine-containing disinfectant （2 000 mg/L and 5 500 mg/L）， and PCR Cleaner， respectively. After 1 min， the contaminated area was wiped with medical cotton swabs and soaked in 300 μL of pure water. After shaking and mixing， 5 μL was taken as a template. The Ct values of ORF1ab and N genes and IC genes of internal standard fragment in the amplified target area of 2019-nCoV after wiping with different disinfection methods were compared to evaluate the effect of eliminating nucleic acid pollution， and each experiment was repeated for three times. Similarly， the effects of ultraviolet irradiation for 0， 1， 2， 3， 4， and 5 hours on the removal of nucleic acid pollution were compared. 【Results】 After 2 000 mg/L and 5 500 mg/L chlorine-containing disinfectant wiped the contaminated area， the Ct values of ORF1ab and N genes and IC genes of internal standard fragment in the amplified target area in 2019-nCoV were all 0， and the Ct values of all genes in the contaminated area in groups 3， 4 and 5 h after UV irradiation were all 0， which completely cleared the pollution and had a strong effect. The effect of PCR Cleaner was second， and 750 mL/L ethanol was the worst. 【Conclusion】 2 000 mg/L and 5 500 mg/L chlorine-containing disinfectant and ultraviolet irradiation for 3 hours have the best effect of eliminating nucleic acid pollution， which is worth popularizing under appropriate conditions.
RESUMO
Objective To detect the changes and clinical significance of the expression of anti‐GPⅡb/Ⅲa and anti‐GPⅠb/ⅠX on anti‐secreting B cells in patient with thrombocytopenia .Methods Expression of anti‐GPⅡb/Ⅲa and anti‐GPⅠb/ⅠX specific autoantibodies in thrombocytopenia were tested with (CBA) .Results There were significantly more circulating anti‐GPⅠ b/ⅠX and anti‐GPⅡb/Ⅲa antibody‐producing B cells in primary ITP(P<0 .05) for all comparisons .For diagnosing primary ITP ,the an‐ti‐GPⅠb/ⅠX had 43% sensitivity and 89% specificity ,whereas the anti‐GPⅡ b/Ⅲ a had 86% sensitivity and 83% specificity . When two tests were combined ,the sensitivity improved to 90% without a reduction in specificity .Conclusion The assay for detec‐ting anti‐GPⅠb/ⅠX is useful for identifying patients with ITP ,but its utility for diagnosing ITP is inferior to the anti‐GPⅡb/Ⅲa assay .
RESUMO
Objective To evaluate the values of combined detection of carbohydrate antigen 125(CA125) ,human epididymis gene protein 4(HE4) ,soluble mesothelin related peptides(SMRP) in early diagnosis for ovarian cancer and the high-risk factors of ovari-an cancer .Methods 43 patients with malignant ovarian cancer were served as the malignant group ,54 patients with benign ovarian tumors as the benign group ,45 healthy women as the control group .Roche Cobas 6000 Automatic Electrochemiluminescence Immu-noassay Analyzer and enzyme-linked immunosorbent assay (ELISA) were adopted to detect serum CA125 ,HE4 ,SMRP levels . High-risk factors of ovarian cancer was subject to Logistic regression analysis .Results Serum levels of CA125 ,HE4 and SMRP of patients in malignant group were significantly higher than those in the benign group and the control group (P<0 .01) .The sensitivi-ties of CA125 ,HE4 and SMRP detection alone for ovarian cancer diagnosis were 73 .3% ,82 .8% and 70 .8% ,respectively ,while their specificities were 79 .1% ,87 .4% and 83 .3% ,respectively .The sensitivity and specificity of joint detection of CA 125 ,HE4 , SMRP for ovarian cancer diagnosis were 90 .2% and 79 .3% ,respectively .Logistic regression analysis showed that age at menarche younger than 13 years ,menstrual cycle less than 30 d and irregular menstruation were the high-risk factors of ovarian cancer ,with their odds ratio(OR) of 2 .11[95% confidence interval(CI):1 .09-4 .10 ,1 .91(95% CI:1 .21-3 .10) and 1 .57(95% CI:0 .83-2 .94) .Conclusion CA125 detection combined with HE4 or SMRP can markedly improve the diagnostic sensitivity for ovarian cancer .
RESUMO
@#ObjectiveTo explore the noxious effect and the extent of damage in nigra-striatum neuron of bilirubin.Methods30 newborn rabbits were divided into 3 groups randomly:control group(group C,12 rabbits) which were injected normal salt intraperitoneally,model group 1(group N1,12 rabbits) which were injected bilirubin 100mg/kg intraperitoneally, model group 2(group N2,12 rabbits) which were injected bilirubin 200mg/kg intraperitoneally. All the rabbits in group C and 6 rabbits in group N1(group N1a) and group 2(group N2a) were killed 6 hours after injection.Other 6 rabbits in group N1(group N1b) and group 2(group N2b) were killed 16 hours after injection. The quantity of neuron in nigra-striatum were determined,and the changes of ultrastructure were observed by electron microscope.ResultsThe neuron in nigra-striatum in group N2b were decreased compared with group C and group N1a(P<0.05),as well as with group N1b and N2a. The ultrastructure of the neuron was changed.ConclusionsThe changes in utrastructure and the quantity of nigra-striatum neuron were associated with the concentration and time of exposure under bilirubin. It is consisted with the changes of ultrastructrue and quantity in nigra-striatum.