Effects of Neuroligin on the Proliferation and Apoptosis of SH-SY5Y Cells / 现代生物医学进展

Objective:To study effects of neuroligin (NLG) on the proliferation and apoptosis in human neuroblastoma SH-SY5Y cells.Methods:The SH-SY5Y cells were cultured in vitro for 24 hours,and then transfected with NLG siRNA at dose of 50,100,200 μmol/L,respectively.MTT procedure was used to detect the cell proliferation,and expression levels of apoptosis gene including Bax or Bcl-2 and Bcl-xl were measured by RT-PCR.Results:Compared to control groups proliferation of SH-SY5Y cells were distinctly inhibited after NLG siRNA transfection accompany with a dose-dependent,which was caused by activation of apoptosis.Conclusions:NLG protect neuron by inhibiting apoptosis.

Application of 3M transparent dressing joint 3MTM CavilonTM No Sting Barrie Film in the skin care after peripherally inserted central catheter / 中国实用护理杂志

Objective To evaluate the skin protection of the 3M transparent dressing joint 3MTM CavilonTM No Sting Barrie Film after peripherally inserted central catheter (PICC).Methods A total of 120 PICC catheter patients with lung cancer (observation time last about 3 months from starting the rearing pipe on the day to pipe-taken) were selected.They were divided into 2 groups by random digits table method with 60 cases each.Control group was given 3M transparent dressings stick in the PICC fixed place skin.Observation group was given 3M transparent dressings paste joint 3MTM CavilonTM No Sting Barrie Film.The incidence of complications in the PICC fixed place skin were observed and compared between 2 groups.Results The skin allergy,phlebitis,skin damage,total complications were 5,4,1,10 cases in control group after PICC catheter and 2,1,3,6 cases in observation group,and there was significant difference in the incidence of the total complications,x2=4.23,P＜0.05.Conclusion Using 3M transparent dressings joint 3MTM CavilonTM No Sting Barrie Film for skin care after PICC catheter,can effectively protect the skin after PICC catheter,reduce the incidence of adverse reactions.

Protective roles and mechanisms of limb ischemic preconditioning and postconditioning in cerebral ischemia / 国际脑血管病杂志

Remote ischemic preconditioning and postconditioning are two new ways to reduce ischemia-reperfusion injury.Studies in recent years have shown that it also has significant protective effect for cerebral ischemia.At the appropriate time to give appropriate strength of remote ischemic preconditioning or postconditioning may induce brain ischemic tolerance and thus produce neuroprotection.The procedures of limb ischemic preconditioning and postconditioning are simple,and the clinical application value is higher.This article reviews the protective effect of limb ischemic preconditioning and postconditioning on cerebral ischemia.