RESUMO
Objective To investigate the expression,clinicopathological significance and correlation of S100A4,EGFR and PI3K in lung adenocarcinoma.Methods Immunohistochemical method (EnVision two steps) was used to detect the expression of S100A4,EGFR and PI3K proteins in 84 lung adenocarcinoma tissue samples and 30 normal lung tissue samples.The relationship of S100A4,EGFR and PI3K expression with clinicopathologic factors,post-operative five-year survival and the correlations among the three proteins were analyzed.Results The positive expression rates of S100A4,EGFR and PI3Kin lung adenocarcinoma tissues were higher than those in normal tissues,respectively [(69.0 ％,58/84) vs (6.7 ％,2/30),(64.3 ％,54/84) vs (16.7 ％,5/30),(52.4 ％,44/84) vs (13.3 ％,4/30),P ＜ 0.01].The expression of S100A4,EGFR and PI3K proteins were positively correlated with the differentiated degree,lymph node metastasis,clinical stages,and five-year survival (P ＜ 0.05),but not correlated with other clinicopathologic factors (P ＞ 0.05).The expression of S100A4 was positively correlated with EGFR and PI3K in lung adenocarcinoma (P ＜ 0.01),and the expression of EGFR was positively correlated with PI3K (P＜ 0.01).Conclusions S100A4,EGFR and PI3K were closely related with the occurrence,development,metastasis and prognosis of lung adenocarcinoma.S100A4 might be an important marker in estimating biological behavior and metastasis tendence of lung adenocarcinoma.S100A4 may be correlated with EGFR and PI3K.
RESUMO
A highly sensitive fluorescence spectroscopic method was established for the selective determination of estradiol, which took advantages of the excellent molecular recognition capability of aptamer and the energy transfer between the specific fluorescent groups. The effects of the pH value, buffer constituent and concentration, the concentration of DNA, the experimental temperature and response time on the detection of estradiol were studied. Under the optimal conditions (50 mmol/L BR buffer solution with pH value at 7. 4, 1. 0×10-7 mol/L for each DNA strand, incubation at 45 ℃, response time 19 min), the change of the fluorescence intensity (ΔI) versus the logarithm of the concentration of estradiol ( lgC) was linear over a concentration range from 1. 0×10-11 mol/L to 5. 0×10-9 mol/L with good linear correlation (r=0. 9953). The limit of detection (LOD) was found to be 6. 0×10-12 mol/L (S/N=3). This method was successfully applied to the detection of estradiol in human urine, with the recovery in the range of 94. 0%-103. 5%. This method showed good precision and accuracy.