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China Pharmacy ; (12): 811-815, 2020.
Artigo em Chinês | WPRIM | ID: wpr-819092

RESUMO

OBJECTIVE:To study the protective effect of timosaponin BⅡ(TB-Ⅱ)on blood vessels and explore its possible mechanism. METHODS :Using aquaculture water as blank control ,the effects of 100,200 and 400 μg/mL TB-Ⅱ treatment for 48 h on the situation of subintestinal veins (SIVs)in normal zebrafish embryos 24 h after fertilization (24 hpf)were investigated. PTK787(0.06 μg/mL),a tyrosine kinase inhibitor ,was used to induce the model of zebrafish intestinal vascular injury ;using combing with 0.1% dimethyl sulfoxide but no PTK 787 as blank control ,combing with PTK 787 but no drug as model control ,the effects treatment of 100,200 and 400 μg/mL TB-Ⅱ for 48 h on the SIVs of zebrafish model with vascular injury were investigated. Relative expressions of fam-like tyrosine kinase 1(Flt-1),kinase insert domain containing receptor (Kdr),kinase insert domain containing receptor l (Kdr-l),vascular endothelial growth factor A (VEGF-A),tumor necrosis factor α(TNF-α)and interleukin 6 (IL-6)mRNA were detected by RT-PCR. RESULTS :100 μg/mL TB-Ⅱ could significantly increase the sprouting vessel of normal zebrafish SIVs sprouting vessel number (P<0.05),and 200 μg/mL TB-Ⅱ could significantly increase SIVs number of normal zebrafish (P<0.05). Compared with blank control , SIVs treatment (P<0.01),and the relative expressions of Flt-l , Kdr,Kdr-l,VEGF-A,TNF-α and IL-6 mRNA were alse decreased significantly (P<0.05 or P<0.01). After treated 化。E-mail:pn333@163.com with different concentrations of TB- Ⅱ ,SIVs number of vascular injury model zebrafish increase d to different extents ;relative expressions of Flt-l ,Kdr,Kdr-l,VEGF-A,TNF-α and IL-6 mRNA were increased to different extents. There was no significant difference in SIVs number and the expression of Flt-l ,TNF-α mRNA in zebrafish treated with 100 μg/mL TB-Ⅱ and the expression of TNF-α mRNA in zebrafish treated with 400 μg/mL TB-Ⅱ, but there was statistical significance in other indexes (P<0.05 or P<0.01). CONCLUSIONS :TB-Ⅱ has a certain function of promoting angiogenesis and repairing damaged blood vessels ,and its mechanism is related to the up-regulation of vascular endothelial growth factor receptor and pro-inflammatory cytokine expression.

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