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0.05).Microscopic morphology has also been observed, no visible damage could be found in the structure of lenses from eyes injected with ouabain (figs 3 and 4). Serial sections of paraffin-embedded lenses show that the number of fiber cells increased significantly in experimental samples treated with ouabain at a concentration of 0.1 ?M(table 4, P
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Objective In order to further study the role of gap junction(GJ) in growth control; and explore the possible mechanisms for the inhibition of GJ formation. Method A monoclonal antibody,called ND6, which is specific for MIP(major intrinsic protein)in the plasma membrane of eye lens fiber cells in chicken,was injected into the right eyes of chicken embryos at development stage 20, while the left eyes were not injected and served as controls. The size of the lenses was measured 24 hours after the ND6 treatment; the protein phosphorylation of the lenses, including intact and homogenized lenses was also analyzed 24 hours after the ND6 treatment. Results The size of the lenses from treated eyes was significantly larger than those of the control ones( P