Relationships between the enrichment of ETBF, Fn, Hp in intestinal and colorectal cancer / 中华肿瘤杂志

Objective@#To explore relationships between the enrichment of ETBF, Fn, Hp in feces, tissues and colorectal cancer.@*Methods@#Feces, lesion tissue and adjacent tissue from 24 patients with colorectal cancer and 31 patients with adenomas were collected, and we collected Feces and tissue of 20 healthy control persons. Then the copy numbers of enterotoxigenic B. fragilis (ETBF), Fusobacterium nucleatum (Fn) and Helicobacter pylori (Hp) were determined by quantitative real-time PCR. Immunohistochemical method was used to examine the expression intensity of EGFR and p53, and the relationships between different expression intensity of EGFR, p53 and the numbers of three bacterias.@*Results@#In the feces, copy numbers of ETBF and Fn were as follous: colorectal cancer group>adenomas group>healthy control group (P<0.05). Copy numbers of Hp were as follous: colorectal cancer group>healthy control group (P<0.01); adenomas group>healthy control group (P<0.01). In the tissue, copy numbers of ETBF, Fn were as follows: colorectal cancer group>adenomas group>healthy control group (P<0.05). Copy numbers of Hp were as follows: colorectal cancer group>healthy control group (P<0.01); adenomas group>healthy control group (P<0.01). Copy numbers of those three bacteria in the lesion tissue and the adjacent tissue had no significant difference. This happened both in colorectal cancer group and adenomas group. The different expression intensity of EGFR, p53 and the number of three bacteria showed no obviously statistical correlation(P>0.05).@*Conclusion@#Adenomatous polyp and colorectal cancer patients show high enrichment of ETBF, Fn and Hp in both feces and tissues. ETBF, Fn and Hp probably contribute to the development of adenomatous polyp and colorectal cancer. Trial registration Chinese Clinical Trial Registry, ChiCTR-BOC-17012509.

Relationship between intestinal mucosa associated microbiota and clinical manifestations in the patients with ulcerative colitis / 中华消化杂志

Objective@#To investigate the changes of the intestinal mucosa-associated microbiota in the patients with ulcerative colitis (UC), and to explore their correlation with the clinical manifestations.@*Methods@#From June to October 2016, at Gastrointestinal Endoscopy Center, the Second Affiliated Hospital of Xi′an Jiaotong University, 28 patients with UC and 16 healthy individuals who underwent colonoscopy examination were enrolled. The mucosa specimens of them were collected for fluorescent in situ hybridization (FISH). The bacterial flora were observed and counted, the correlation between the bacterial flora and the clinical manifestations were analyzed. Kruskal-Wallis test and Spearman rank correlation analysis were performed for statistical analysis.@*Results@#Among 28 patients with UC, 16 were at active phase and 12 at remission phase. The number of total bacteria flora, Escherichia coli, Clostridium and Bacteroides of the active UC group and remission UC group were all more than those of healthy control group; however the number of Lactobacillus and Bifidobacteria were less than those of healthy control group, and the differences were statistically significant (χ2=23.34, 19.94; 23.40, 12.96; 23.39, 19.16; 23.32, 10.46; 23.19, 4.25; 18.94, 12.33; all P<0.05). The number of total bacteria flora, Escherichia coli and Bacteroides of the active UC group were more than those of the remission UC group, however the number of Lactobacillus and Bifidobacteria were less than those of the remission UC group, and the differences were statistically significant (χ2=7.32, 5.63, 5.62, 20.38 and 4.82; all P<0.05). In the patients with UC, the defecation frequency was positively correlated with the count of Bacteroides (r=0.459, P=0.014) and was negatively correlated with the count of Lactobacillus (r=-0.634, P<0.01). In UC patients, bloody stool, endoscopic appearance and total Mayo score were positively correlated with the counts of universal bacteria, Escherichia coli and Bacteroides (r=0.469, 0.403, 0.376; 0.604, 0.562, 0.475; 0.551, 0.463, 0.461; all P <0.05); and which were negatively correlated with Lactobacillus and Bifidobacteria (r=-0.570, -0.413; -0.899, -0.458; -0.862, -0.480; all P<0.05). The evaluation by the doctors was positively correlated with the counts of Escherichia coli (r=0.415, P=0.028), however was negatively correlated with Lactobacillus and Bifidobacteri (r=-0.841, -0.529; both P<0.01).@*Conclusion@#The intestinal microbiota have sigificantly changed in patients with UC which are correlated with some clinical manifestations of UC.

Relationship between intestinal mucosa associated microbiota and clinical manifestations in the patients with ulcerativecolitis / 中华消化杂志

Objective To investigate the changes of the intestinal mucosa-associated microbiota in the patients with ulcerative colitis (UC) ,and to explore their correlation with the clinical manifestations . Methods From June to October 2016 , at Gastrointestinal Endoscopy Center , the Second Affiliated Hospital of Xi'an Jiaotong University ,28 patients with UC and 16 healthy individuals who underwent colonoscopy examination were enrolled .The mucosa specimens of them were collected for fluorescent in situ hybridization (FISH) .The bacterial flora were observed and counted ,the correlation between the bacterial flora and the clinical manifestations were analyzed . Kruskal-Wallis test and Spearman rank correlation analysis were performed for statistical analysis .Results Among 28 patients with UC ,16 were at active phase and 12 at remission phase . The number of total bacteria flora , Escherichia coli , Clostridium and Bacteroides of the active UC group and remission UC group were all more than those of healthy control group ;however the number of Lactobacillus and Bifidobacteria were less than those of healthy control group ,and the differences were statistically significant (χ2 = 23 .34 ,19 .94;23 .40 , 12 .96;23 .39 ,19 .16 ;23 .32 ,10 .46 ;23 .19 ,4 .25;18 .94 ,12 .33;all P<0 .05) .The number of total bacteria flora , Escherichia coli and Bacteroides of the active UC group were more than those of the remission UC group ,however the number of L actobacillus and Bi f idobacteria were less than those of the remission UC group ,and the differences were statistically significant (χ2 =7 .32 ,5 .63 ,5 .62 ,20 .38 and 4 .82 ;all P<0 .05) .In the patients with UC ,the defecation frequency was positively correlated with the count of Bacteroides (r=0 .459 ,P=0 .014) and was negatively correlated with the count of Lactobacillus (r= -0 .634 ,P<0 .01) .In UC patients ,bloody stool ,endoscopic appearance and total Mayo score were positively correlated with the counts of universal bacteria ,Escherichia coli and Bacteroides (r=0 .469 , 0 .403 ,0 .376 ;0 .604 ,0 .562 ,0 .475;0 .551 ,0 .463 ,0 .461 ;all P <0 .05);and which were negatively correlated with Lactobacillus and Bif idobacteria (r= -0 .570 ,-0 .413 ;-0 .899 ,-0 .458;-0 .862 ,-0 .480 ;all P< 0 .05) .The evaluation by the doctors was positively correlated with the counts of Escherichia coli (r= 0 .415 , P= 0 .028) ,however was negatively correlated with Lactobacillus and Bifidobacteri (r= -0 .841 , -0 .529 ;both P< 0 .01) .Conclusion The intestinal microbiota have sigificantly changed in patients with UC which are correlated with some clinical manifestations of UC .