RESUMO
OBJECTIVE:To optimize the extraction technology of Compound Rhodiolae crenulatae granule. METHODS:The pharmacodynamic index of learning and memory ability was used to screen the water extraction,water extraction and alcohol pre-cipitation and alcohol extraction methods for Compound R. crenulatae granule in mice by Y-type water maze test. Using the compre-hensive scores of salidroside,ferulic acid extraction amount and extraction yield as indexes,orthogonal test was used to optimize the ethanol volume fraction,extraction time,solvent amount and extraction times,and verification test was conducted. RESULTS:Products by alcohol extraction can significantly improve learning and memory ability in the 3 methods;the optimal extraction tech-nology was confirmed to 8-fold 70% ethanol reflux extracting for 3 times,1.0 h each time. In verification test,average extraction amount of salidroside and ferulic acid were 4.884 mg/g and 1.669 mg/g,and average extraction yield was 33.52%(all RSD<2%, n=3). CONCLUSIONS:The optimized extraction technology is stable,reliable,simple and feasible,which can be used for indus-trial extraction of Compound R. crenulatae granule.
RESUMO
OBJECTIVE: To study the influence factors of the oxidation of DHA and EPA,and to find effective antioxidants.METHODS: The oxidation of DHA and EPA was optimized by orthogonal test with the quality of DHA and EPA as indicators and temperature,light and time as factors.The optimized antioxidants were selected by adding salidroside,BHA,BHT,vitamin E into samples.RESULTS: If there is lower temperature,weaker light and shorter storage,the oxidation of DHA and EPA will be more slowly.Effect of salidroside on the oxidation of DHA and EPA is the strongest.CONCLUSION: The oxidation of DHA and EPA can be reduced by lowering temperature,storing in dark place,reducing storage duration and adding salidroside.
RESUMO
Paeoniflorin in antiaging Preparation was qualitatively identified with chemical and TLC methods and quantitatively determined with HPLC. The results showed both tne chemical and TLC method gave fast and accurate results and HPLC was an excellent for quantitative determination with an average recovery of 100.72% and coefficient of variation 1.22%, which is suitable for the quality control of the preparation.