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Objective To evaluate the nutritional value of yeast protein, and to provide laboratory evidence for its application in the field of food and health food. Methods The amino acids content of yeast protein, soybean protein, whey protein and mixed protein was detected. Five nutritional evaluation methods, including amino acid score, ratio of total essential amino acid, ratio coefficient of amino acid, Pearson's correlation coefficient, and equally weighted gray correlation analysis were used to evaluate the protein quality. The protein efficacy test of the four proteins was performed in Sprague-Dawley rats to compare their nutritional value in vivo. Results The results showed that yeast protein was rich in essential amino acids, accounting for 47.58% of the total amino acids, and the ratio of essential amino acids to non-essential amino acids reached 0.91. Five nutritional evaluation methods all revealed that the yeast protein was an excellent protein in quality. The results of the protein efficacy test showed that yeast protein was able to promote the growth and development of the animals, and keep them in good nutritional condition, which was similar to whey protein, soy protein, and casein. The mixed protein had the highest corrected efficiency ratio among all the proteins tested in this study, possibly because the mixed ingredients made the amino acids complementary and thus had higher nutritional value. Conclusion Yeast protein can be used as a useful supplement to plant-derived and animal-derived protein products.
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Objective To explore the clinical value of high frequency color doppler ultrasound in the diagnosis of lower limb artery pathological changes of diabetes.Methods Linear array of high frequency probe was used in regular line double lower limbs artery ultrasound examination in 102 cases with diabetes (observation group) and 98 healthy people(control group).The lower limb artery(including femoral artery,popliteal artery,posterior tibial artery,the tibia and dorsalis pedis artery)before the intima-media thickness (IMT)were measured,and the presence of plaque,the plaque nature,the degree of luminal stenosis or occlusion of the judgment and hemodynamic changes were observed.Results In observation group,the femoral artery plaque detection rate was 22％,popliteal artery plaque detection rate was 36％,posterior tibial artery plaque detection rate was 39％,pretibial artery plaque detection rate was 38％,dorsalis pedis artery plaque detection rate was 45％.In control group,the femoral artery plaque detection rate was 9％,popliteal artery plaque detection rate was 7％,posterior tibial artery plaque detection rate was 10％,pretibial artery plaque detection rate was 13％,dorsalis pedis artery plaque detection rate was 16％.In the observation group,lower limb artery plaque caused mild stenosis in 31 cases,moderate stenosis 16 cases,severe stenosis 11 cases,block 4 cases.In the control group,9 cases had mild stenosis caused by lower limb artery plaque,moderate stenosis 5 cases,3 cases with severe stenosis and occlusion in 0 case.In the observation group,lower limb artery soft spot 14,hard spots in 19,41 mixed plaques.In the control group,lower limb artery soft spot 4,hard spot 8,11 mixed plaques.Conclusion Lower limb artery pathological changes is closely related with diabetes,especially the artery stenosis and block the knee can predict the development of diabetes.
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BACKGROUND:There are many methods for dental impression disinfection, including ultraviolet disinfection method, spraying, immerses disinfection method, argon plasma jet and radio frequency glow discharge on the argon gas ionization disinfection method. At present, there is stil lack of studies addressing the disinfection effect after gargling with mouthwash. OBJECTIVE:To detect the kil ing effect on bacteria and fungi from the dental impressions, which are taken after gargling with cetylpyridinium chloride gargle and stable chlorine dioxide gargle, respectively. METHODS:One hundred patients were randomly divided into cetylpyridinium chloride gargle group and stable chlorine dioxide gargle group. They were told to gargle with clean water for 1 minute, and then impressions were taken to remove the models with sterile cotton swab sampling in neutralizing agent which were sent to laboratory for bacteria and fungi culture. After 1 hour, the patients were asked to gargle with the different mouthwashes for 1 minute again, and then sterile cotton swab sampling was done at the same position for bacteria and fungi culture. The number of colonies was recorded, and the kil ing effects on kil ing bacteria and fungi on the surface of the impressions before and after gargling with different mouthwashes were observed. RESULTS AND CONCLUSION:The number of bacteria and fungi before gargal had no significance difference before gargling. After gargling, the number of bacteria and fungi on the model was significantly lower than before (P<0.001). Two groups did not appear with any adverse reactions, and patients felt refreshed and comfortable after gargling with mouthwash. To gargle with mouthwash before taking impressions can control the number of bacteria and fungi on the samples effectively, thereby achieving the aim of control ing the mutual infections between the doctor and the patients effectively.
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Objective: To investigate the effect of pricking blood therapy on behavior and brainstem c-fos and c-jun gene expression in migraine rats. Methods: A rat model of migraine was made with nitroglycerol. The rats were treated by pricking blood. Rat behavior was observed. Brainstem c-fos and c-jun gene expression was examined by immunohistochemistry. Results: Ear redness improved significantly, the number of head scratchings decreased obviously (P<0.05) and c-fos and c-jun expression was reduced markedly (P<0.01) in the treatment group after pricking blood compared with the model group and the blank group. Conclusion: Pricking blood treatment can improve behavior indices and reduce c-fos and c-jun positive expression in migraine rats.