Effect of different doses of propofol for children anesthesia on depth, stress response and hemodynamic / 中国现代医生

Objective To discuss effect of different doses of propofol for children anesthesia on depth, stress response and hemodynamic. Methods Clinical data of 60 cases with abdominal surgery were retrospectively analyzed. All cases were divided into 3 groups according to different doses of propofol. Group 1 of 21 cases, initial maintenance dose of propofol was 10 mg/(kg·h), then every 20 minutes reduced 2 mg/(kg·h), with 6 mg/(kg·h)maintaining to sew leather;Group 2 of 19 cases, initial maintenance dose of propofol was 15 mg/(kg·h)after 20 minutes reduced to 10 mg/(kg·h), after 40 minutes reduced to 8 mg/(kg·h)and maintained to sew leather; Group 3 of 20 cases, initial maintenance dose of propofol was 20 mg/(kg·h), then every 20 minutes reduced 5 mg/(kg·h), with 10 mg/(kg·h) maintaining to sew leather. BIS, hemodynamic and stress response of three groups in different time were compared. Results The depth of anesthesia of group 2 and group 3 at each time point were greater than in group 1(P<0.01). MAP and heart rate of group 3 were significantly inhibited. Stress response in group 1 was more intensity than group 2 and group 3 (P<0.05). Conclusion Propofol anesthesia for children with 15 mg/(kg·h), 10 mg/(kg·h), 8 mg/(kg·h) to maintain anesthesia, children can achieve satisfactory depth of anesthesia, and the impact on the stress response and hemodynamic are lighter.

Spontaneous senescence in malignant tumor cell lines / 肿瘤

Objective:To explore whether spontaneous sene-scence widely existed in malignant tumor cells. Methods:Sene-scence-associated beta-galactosidase (SA-β-Gal) staining kit was used to detect the activity of SA-β-Gal in ten different malignant tumor cell lines before and after serum deprivation. Results:SA-β-Gal was expressed in some cells of 10 malignant tumor cell lines during exponential growth phase without any treatment. However, the percentage of senescent cells was significantly different among them, the lowest expression was observed in HeLa cell line (0.65%), and the highest expression was seen in HepG2 cell line (3.69 %, F=13.006, P= 0.000). Furthermore, not all the SA-β-Gal positive aging cells were polyploid cells. After 24-h serum deprivation, the number of SA-β-Gal positive cells was significantly increased (P=0.001). Conclusion:These findings indicate that immortal malignant tumor cell lines could undergo spontaneous senescence but the level was different between various cell lines. Short-term serum de-privation significantly increased the percentage of aging cells indicating that serum deprivation-induced cell senescence may be a rapid, easy, and effective way for anti-tumor therapy.

Design and implementation of a new type excitation source and the optimal excitation coil for MIT / 生物医学工程学杂志

This research work was aimed to improve the performance of magnetic induction tomography (MIT) system by designing a high-performance excitation source and the optimal excitation coil. A new type excitation source with adjustable output was designed, and then the power circuit was simulated by the software ICPA. Focused and solenoid coils were designed in accordance with the design principle of coil, then the optimal excitation coil was proved by measuring the magnetic field distribution and the experimentation of phase detection using neuron cell models. At the stated excitation frequency, the parameters of the excitation source are output power 0.035 W-31.4 W, steady output peak current over 1 A, frequency stability 10(-9), and THD amplitude less than -51dB. When compared with other coils, the focused discal excitation coil is most effective for phase detection with the use of neuron cell models. The excitation source can produce the stated frequency sine wave with higher frequency stability, lower THD and wider adjustable output power. The phase difference between normal cell model and edema cell model was more significant by measurement using focused discal coil.

Impacts of high power microwave radiation on the apoptosis of testicular germ cell in the rat / 医学研究生学报

Objective:To study the impacts of high power microwave radiation on the testicular germ cell apoptosis in the rats. Methods: Fifty Spraque-Dawley rats were randomly divided into two groups .In the experimental group,the animals were radiated with S wave band 20 mW/cm 2 high power microwave for 5 min, and in the control group,5 rat were not radiated. 5 blank radiation groups served as controls. At the end of process, testicular germ cell apoptosis were detected by in situ terminal deoxymudeotityl transferase mediated dUTP nick end labeling (TUNEL) 6 h , 24 h, 48 h, 72 h and 5d following radiation. Results:In experiment group the amounts of apoptosis cells were more than that of control group 6 h?24 h?48 h following radiation(P0.05). Conclusion: Radiation by HPM for 5 min would result in testicular germ cell apoptosis and damage in the rat.

Immunohistochemical study of related inflammatory cytokines in epiretinal membrane / 眼科新进展

Objective　To investigate the expression and role of inflammatory cytokines like interleukin-1β(IL-1β)、interleukin-6(IL-6)、interleukin-8(IL-8) and tumor necrosis factor-alpha(TNF-α)in epiretinal membranes(ERM)of eyes with proliferative vitreoretinopathy(PVR).Methods　Nineteen epiretinal membranes were obtained from eyes undergoing vitrectomy for retinal detachment complicated with PVR and observed by immunohistochemical methods.Results　Expression of IL-1β、IL-6、IL-8 and TNF-α were observed in 9、12、11and 15 membranes respectively, with positive staining mostly in the extracellular matrix of epiretinal membranes. Only one membrane showed positive to IL-6 intracellularly, expression for all the cytokines simultaneously in 4 membranes.Conclusion　The findings indicate that cytokine-mediated pathways of inflammation are involved in the pathogenesis of PVR.

The expression of anti-apoptosis gene bag-1 and its relation to the differentiation of intrahepatic cholangiocarcinoma / 中国普通外科杂志

Objective To investigate the expression of anti-apoptosis gene bag-1 and its relation to the differentiation of intrahepatic cholangiocarcinoma (ICC). Methods Immunohistochemical techniques were adopted to study the expression of bag-1 in ICC tissue ( n=48) and para-hepatocarcinoma bile duct ( control group, n=25). Results Expression of bag-1 in the ICC group was significantly higher than that in the control group. In the ICC group (P