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1.
Artigo em Inglês | IMSEAR | ID: sea-136276

RESUMO

Burkholderia pseudomallei is the causative agent of melioidosis, and its infection usually affects pa-tients’ lungs. The organism is a facultative intracellular Gram-negative bacillus commonly found in soil and water in endemic tropical regions. Another closely related Burkholderia species found in soil and water is B. thailandensis. This bacterium is a non-pathogenic environmental saprophyte. B. pseudomallei is considerably more efficient than B. thailandensis in host cell invasion and adherence. A previous study by our group demonstrated that after suc-cessfully invading cells, there was no difference in the ability to survive and to replicate between both Burkholderia species in cultured A549 human lung epithelial cells. In this study, Human Affymetrix GeneChips were used to identify the difference in gene expression profiles of A549 cells after a 2-h exposure to B. pseudomallei and B. thai-landensis. A total of 280 of 22,283 genes were expressed at higher levels in the B. pseudomallei-infected cells than in the B. thailandensis-infected cells, while 280 genes were expressed at lower levels in the B. pseudomallei-infected cells. Approximately 9% of these genes were involved in immune response and apoptosis. Those genes were further selected for gene expression analysis using reverse transcription PCR and/or real-time RT-PCR. The results of RT-PCR and real-time RT-PCR are in accordance with data from the microarray data in that bcl2 gene expression in the B. pseudomallei-infected cells was 2-fold higher than the level in the B. thailandensis-infected cells even though no apoptosis was seen in the infected cells. The levels of E-selectin, ICAM-1, IL-11, IRF-1, IL-6, IL-1 and LIF genes expression in the B. pseudomallei-infected cells were 1.5-5 times lower than in the B. thailan-densis-infected cells. However, both species stimulated the same level of IL-8 production from the tested epithelial cell line, and no difference in the ratio of adherent polymorphonuclear cells (PMNs) to infected A549 cells of both species was observed. Taken together, our results suggest that B. pseudomallei manipulates host response in fa-vor of its survival in the host cell, which may explain the more virulent characteristics of B. pseudomallei when compared with B. thailandensis.

2.
Asian Pac J Allergy Immunol ; 2002 Sep; 20(3): 161-6
Artigo em Inglês | IMSEAR | ID: sea-36732

RESUMO

Nonstructural 3 (NS3) protein of hepatitis C virus (HCV) is one of the antigens commonly used in diagnostic assays for antibody to hepatitis C virus. However, immune response to the NS3 protein from one genotype may not cross-react with that from other genotypes. In the development of an anti-HCV assay, the NS3 genes from genotypes 1 and 3 commonly found in Thailand were amplified and cloned into a bacterial expression system. These recombinant NS3 proteins were immunogenic and reacted with plasma samples of Thai patients infected with various HCV genotypes. Interestingly, the NS3 proteins from the Thai genotypes could react with 3 plasma samples from HCV infected Thai blood donors, which could not bind to the NS3.1 protein in the commercial HCV immunoblot kit using antigen from HCV genotype 1. This finding supports our prior observation that the appropriate HCV antigens used in a diagnostic assay should be derived from the virus genotypes commonly found in that geographical region.


Assuntos
Sequência de Aminoácidos , Sequência de Bases , Biomarcadores/sangue , Primers do DNA/genética , DNA Viral/genética , Eletroforese em Gel de Poliacrilamida , Vetores Genéticos/genética , Genótipo , Hepacivirus/química , Hepatite C/diagnóstico , Humanos , Immunoblotting , Dados de Sequência Molecular , Técnicas de Amplificação de Ácido Nucleico , Isoformas de Proteínas/genética , Proteínas Recombinantes/diagnóstico , Sensibilidade e Especificidade , Tailândia , Proteínas do Core Viral/genética , Proteínas não Estruturais Virais/diagnóstico
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