RESUMO
Sm8 is a major tegumental antigen of Schistosoma mansoni. The partial cDNA was isolated and analyzed. Sequence analysis revealed transmembrane compatible hydrophobic domains and a putative leucine zipper pattern. The mRNA and the protein are predominantly expressed in adult worms
Assuntos
Animais , Antígenos de Helmintos , Proteínas de Helminto , Schistosoma mansoni , Sequência de Aminoácidos , Antígenos de Helmintos , Sequência de Bases , Western Blotting , DNA Complementar , Dados de Sequência Molecular , RNA de HelmintosRESUMO
Sm15 and Sm13 are recognized by antibodies from mice protectely vaccinated with tegumental membranes, suggesting a potencial role in protective immunity. In order to raise antibodies for immunochemical investigations, the genes for these antigens were expressed in pGEX and pMAL vectors so that comparisons could be made among different expression systems and different genes. The fusion proteins corresponding to several parts of the gene for the precursor of Sm15 failed in producing antibodies recognizing the parasite counterpart. On the other hand, antibodies raised against Sm13 MBP-fusion proteins recognized the 13 kDa tegumental protein. Thus the peculiarities of the gene of interest are important and the choice of the expression system must sometimes be decided on an impirical basis.